**4.17 Pesticides in fruit and vegetables**

Fruit and vegetables are important crops of horticulture, as they are an integral part of the human diet. Fruit and vegetables provide carbohydrate, protein, vitamins, minerals, fiber and help in the maintenance of a healthy life style. However, in current years demand of fruit and vegetables has increased tremendously, because of high consumption and population demand. Therefore to boost the production, farmers are using so many chemicals in terms insecticides, fungicides, herbicides, acaricides, and rodenticides for prophylactic use or in diseased condition. However, it has been reported that, these chemical has very harmful effect on human health [52]. Savini et al. [33] reported a quick and sensitive UPLC method coupled with Orbitrap for determining highly polar pesticides and contaminants in processed fruits and vegetables.

## **4.18 Analysis of food-based dyes using UPLC-MS**

Synthetic oil-soluble mono-azo coloring agents such as Sudan dyes and Para Red are very common in food industries. Due to minimal expense and high intensity color it is very commonly used as food additives particularly in chili. However, International Agency for Research on Cancer (IARC 1975) categorized these dyes a potential cancer-causing agent. Moreover, illegal use of these dye such as Sudan Red 7B, Sudan I–IV and Para Red have been still found in food impacting consumer health. UPLC-MS has been reported as one of best choice of instrument analysis of such dyes due to their highest sensitivity.

**45**

India

**Author details**

Syed Amir Ashraf<sup>1</sup>

, Sadaf Nazir2

Zulfiqarur Rashid Azaz Ahmad Azad4

of Hail, Hail, Kingdom of Saudi Arabia

University, Aligarh, Uttar Pradesh, India

provided the original work is properly cited.

\*Address all correspondence to: zrazad@gmail.com

, Mohd Adnan3

1 Department of Clinical Nutrition, College of Applied Medical Science, University

2 Department of Food Technology, Bundelkhand University, Jhansi, Uttar Pradesh,

3 Department of Biology, College of Science, University of Hail, Hail, Saudi Arabia

© 2020 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

4 Department of Post Harvest Engineering and Technology, Aligarh Muslim

\*

and

*UPLC-MS: An Emerging Novel Technology and Its Application in Food Safety*

*DOI: http://dx.doi.org/10.5772/intechopen.92455*

*UPLC-MS: An Emerging Novel Technology and Its Application in Food Safety DOI: http://dx.doi.org/10.5772/intechopen.92455*
