**2.2 Equipments**

Magnetic stirrer (C-MAG HS7, IKA, USA), Analytical digital lab balance (Citizen Enterprises, Delhi, India), Fourier transform infrared spectrophotometer (FTIR 8400 s Shimadzu Corporation, Tokyo, Japan), Bard Parker blade number 24, Autoclaved sterile dissecting scissors, Autoclaved sterile jar (Borosil, India), Sterile measuring cylinders (Borosil, India), Dishes (Borosil, India) and Protective equipment such as surgical gloves and surgical autoclaved instruments were used.
