*Promising Techniques for Wastewater Treatment and Water Quality Assessment*


#### **Table 2.**

*Minimal salt media mixture [34].*

stock and stirred to mix it well. Finally, the agar solution was poured into agar plates forming double layer and stored in fridge at 4 °C for further use.

## **4.3 Enrichment culture and isolation of degrading bacteria**

### *4.3.1 First enrichment culture*

Enrichment cultures were undertaken where 2 ml of sample were added into test tube with 8 ml of Minimal Salt Media(MSM) and 0.05%(w/v) substrate(carbazole). The test tubes were then incubated at 30 °C and shaken at 200 rpm until color change was observed indicating heterocyclic hydrocarbon degradation.
