*5.4.2 Influence of nitrogen-containing compounds*

The same procedure adopted for glucidic substances has been followed for M9 nutritive standard substrate, modified with nitrogen - containing compounds. 3 liters of nitrogen-containing mineral substrate (ammonium chloride) have been prepared, notably 1 liter at 0.1%, 1 liter at 0.2% and 1 liter at 0.4%, and 1 liter of organic nitrogen-containing substance (pancreatic digest of casein) at 0.5%. In


#### **Table 2.** *M9 medium – liter<sup>1</sup>*

*.*


**Table 3.** *Bowin fixative.* *Experimental Investigation of Biomass Attachment to Wastewater Reactors DOI: http://dx.doi.org/10.5772/intechopen.94426*

