*5.7.1 Gram-reaction test*

Gram reaction has been accomplished according to Gregersen [30], adopting a 3% KOH solution, wherein one colony is dissolved and then the loopful is removed and checked for viscosity. A significant viscosity change is indicative of Gram negativity, whereas an unchanged viscosity reveals the Gram positivity. The rationale is based on Gregersen's observation that Gram+ withstand potassium hydroxide whereas in Gram- cellular wall is destroyed and DNA is released resulting in a viscosity change.
