**4. Conclusions**

The present investigation made possible an analysis on how the ozone-based oxidative processes influence the reduction of the ecotoxicity risk caused by emergent pollutants. The obtained results have showed algae's behavioral changes among the four examined laboratories–hormones, solids, dermo-cosmetics and psychotropics–, comparing raw effluent samples, treated effluent samples and control.

Significant alterations on *Euglena gracillis* behavior were observed for the effluents from the laboratories of hormones and dermo-cosmetics, with modifications in general speed and velocity of surface ascent. The psychotropics and solids laboratories have not presented significant statistically difference over the algae's behavior. However, complementary studies are appropriate, in order to confirm the long-term toxicity, since the algae find compensatory mechanisms to fit the adversities.

The variations on algae's behavior before the exposure to different pollutants have suggested it is important to distinguish the effluent treatment, according with the characteristics of the substances manipulated in each laboratory, to reduce the environmental toxicity risks.

Therefore, the found biomonitoring data were relevant for better knowing and to be more aware of the issue, indicating the environmental toxicity caused by the effluents from magistral pharmacies may provoke great impact to the environment if revisions of actions and legislation are not performed in a way that in long term the environmental points related to this type of emergent pollutants are reduced.

For future studies, the evaluation of fish's behavior, before similar conditions, may point out better comprehension over the influence of pharmaceutical ecosystem risks.

### **Acknowledgements**

The researchers thank Univille and the Research Support Funds, that make possible the Environmental Impacts Integrated Project (ECOSAM) development.
