*3.7.1.1 Study of the cytotoxic action of organic-inorganic microspheres*

The investigation of cell vitality is a characteristic indicator and a necessary condition in cases of cytotoxic activity of polymeric inorganic-organic microspheres where they will be used in materials that will come into direct contact with humans, such as cement. The MTT method studies the cytotoxic activity of the microspheres that were compositional in the HEK-293 cells. Studied, the cytotoxic action of microspheres at various concentrations. The rates of cell vitality after incubation with these materials are shown in the **Figure 14**. The diagram shows that the microspheres studied are not toxic to humans. In general, we conclude that regardless of polymer concentration the metabolic function of cells is not affected in the presence of micro-transfer systems.

### *3.7.1.2 Toxicity study of organic-inorganic microbeads to St. aureus*

After it has been shown that microspheres are not toxic to humans, it was then investigated whether microspheres affect the development of the microorganism. In other words, quality control is carried out on the toxicity of micro-transport systems in *St. aureus*. Quality control is done by vaccinating the microorganism in Petri plates, in the presence of microspheres. In this case, however, before vaccinating the microorganism in the dish, four *St. aureus* solutions were prepared to which a quantity of each case of microspheres was added to each. The resulting sediment, consisting of microorganisms and microspheres, was inoculated in a Petri dish. Thus, the development of *St. aureus* was observed in an environment containing micro-transport systems. **Figure 17** shows photos of the Petri plates of *St. aureus* for each case of microcephaly studied.

According to **Figure 17**, it is certified that microspheres do not affect the development of *St. aureus*. The colonies formed by the microorganism are visible in all cases of plates. These colonies prove that *St. aureus* remains active metabolically even in the presence of microspheres. This quality control finally shows that the four species of micropesties are not toxic to the microorganism and do not affect it in its development.

The next check concerns whether the presence of microspheres is affected by precipitation CaCO3 by *St. aureus*.

*Self-Healing of Concrete through Ceramic Nanocontainers Loaded with Corrosion Inhibitors… DOI: http://dx.doi.org/10.5772/intechopen.93514*

### **Figure 17.**

*Development of St. aureus in petri plates in the presence of (A) PMMA microspheres, (B) SiO2 microspheres, (C) water traps, and (D) SiO2 coated water microtraps***.**
