**Abstract**

Surface plasmon resonance (SPR) based biosensors have been enormously studied in the last decade for their better sensitivity. In recent years hybrid heterostructures are getting popularity to implement these SPR biosensors for their superior sensing capability. This chapter demonstrates the details of SPR technology with two recently studied prism-based hybrid heterostructures. These heterostructures are made up of conventional SPR biosensors with two additional layers of recently invented transition metal dichalcogenides, platinum di-selenide (PtSe2), and highly sensitive 2D material, tungsten di-sulfide (WS2). Angular interrogation method is discussed to investigate the sensing capabilities of the sensors which prove the superiority of the Ag-PtSe2-WS2 structure. The sensing capability of this structure has been found at least 1.67 times higher than that of the conventional non-hybrid structures, respectively, with comparable FOM and QF. A comparison table has been provided at the end of this chapter which also shows the impressive performance of the hybrid heterostructures for SPR biosensors. Proper demonstration with a suitable example of this chapter will emphasize the potential use of hybrid heterostructure based SPR biosensors in prospective medical diagnostics and biomedical detection applications.

**Keywords:** biosensor, hybrid heterostructure, sensitivity, surface plasmon resonance
