**Conflict of interest**

"The authors declare no conflict of interest."

*Genetic Variability through Induced Mutation DOI: http://dx.doi.org/10.5772/intechopen.95027*

*Genetic Variation*

keep on to reduce [111].

**4. Conclusions**

**Acknowledgements**

**Conflict of interest**

analysis of EMS mutagenesis.

(*Indica* and *Japonica*) by SNPs genotyping [120].

tion of genetic diversity of rice mutants.

"The authors declare no conflict of interest."

multiplexed SNPs genotyping analysis is necessary to utilize the huge SNPs source for high-throughput genetic test in rice [110]. It will become routine to re-sequence the plants genome with current SNPs platforms as the price of genome sequencing

SNPs genotyping have been applied in many organism including rice [112–114], *Arabidopsis* [115], maize [116], soybean [117] and wheat [118]. The high class order of the rice genome has offered genome-wide SNPs source [119]. Polymorphic loci (5.41 million) were detected between the two main domesticated rice subspecies

A complete map of rice genome builds 6,119,311 SNPs variants for 1529 genome orders. SNPs (213,188) were located in *Indica* and *Japonica* rice. Asian and African rice were established 9595 SNPs [121]. Three thousand rice genomes project [122] submit for rice clustering of aus/boro genotypes. Only 208 accessions are categorized as aus/boro depends upon SNPs markers of 200,000. It is also exposed from further study that aus group was genetically related by 376,000 SNPs markers [123].

The considerable amount of phenotypic variability can be identified by employing highly sophisticated Molecular approaches like SSR and SNPs within the mutant populations. These genetic changes indicated that EMS might be helpful for the development of desired genetic changes in crop plants. It was also recommended that current SSRs and SNPs markers could be suited in further analysis for estima-

The authors acknowledge Higher Education Commission (HEC), Islamabad, Pakistan for providing funds for research activities at Genomics lab, CABB. The authors are thankful to Plant Breeding Division (NIAB) for providing assistance in

**16**
