**Abstract**

Examination of ticks collected from ehrlichiosis positive dogs revealed the occurrence of *Rhipicephalus sanguineus*. The distribution of ehrlichiosis in dogs is related to the spreading of vectors. *Ehrlichia canis* is the etiologic agent of canine monocytic ehrlichiosis (CME) and recognized as the most prevalent tick-borne disease affecting dogs and is transmitted by the brown dog tick *Rhipicephalus sanguineus* with an expanding global distribution. Infection of the vertebrate host occurred when an infected tick ingested a blood meal which in turn contaminated the feeding site with its salivary secretion. Blood transfusions from infected donors can also transmit the organisms. Hence, identification of ticks is necessary to detect the disease affecting dogs.

**Keywords:** ticks, ehrlichiosis, Monocytosis, canine, *Rhipicephalus sanguineus*

### **1. Introduction**

Ehrlichial diseases have emerged as significant problems for human and animals over the past two decades [1–3]. In 1935, *Ehrlichia canis* was first discovered in dog in Algeria [4]. Before the outbreak in military working dogs in Southeast Asia in 1967, canine ehrlichiosis was considered to be a mild disease characterized by fever, vomiting and naso-ocular discharge [5]. Since then, the disease in dogs has spread worldwide [6] and caused serious effects.

Canine ehrlichiosis or tropical pancytopenia is an acute, subacute or chronic tick borne disease caused by *E. canis* which is a Gram-negative intracellular bacterium [7] which occurs particularly in tropical and subtropical regions due to its geographical distribution of its vector tick *Rhipicephalus sanguineus* [8]. This disease is characterized clinically by anorexia, fever, vomiting, loss of weight, enlargement of the liver, spleen and lymph nodes, epistaxis, superficial bleeding and thrombocytopenia [9]. Dogs with canine monocytic ehrlichiosis may die due to hemorrhage and/ or secondary infection [10]. Hematological changes in dogs affected with *E. canis* of all stages of infection include a reduction in hematocrit, hemoglobin concentration, blood cell count, thrombocytopenia and leucopenia. However, poor reticulocyte response (non-regenerative anemia) is associated with chronic ehrlichiosis [11]. Diagnosis of ehrlichiosis can be made based on clinical signs, demonstration of morulae in the monocytes, serological testing with the detection of antibodies against *E. canis* and polymerase chain reaction (PCR). Demonstration of morulae inclusions in blood smears of dogs in the subclinical and chronic stages of the disease was often difficult or impossible and has a low sensitivity rate as this

organism is usually present in very low concentrations and hence cannot be used for diagnosis of the disease [10]. The evolutionary thesis suggests that both ixodid and argasid ticks have been in existence since the late Paleozoic to early Mesozoic eras [12]. Antecedent forms evolved as obligate ectoparasites of smooth-skinned reptiles during the late Paleozoic era [5].

Ticks are the most important ectoparasites in tropical and sub-tropical areas. They are also responsible for severe economic losses either through direct effects of blood sucking or indirectly as vectors of pathogens and toxins. Ticks (Acari: Ixodida) are blood feeding ectoparasites acts as vectors of human diseases next to mosquitoes, but comparatively more important as vectors of animal diseases [13–15]. Ticks belong to

Phylum: Arthropoda Class: Arachnida Subclass: Acari Order: Parasitiformes Suborder: Ixodida

Ixodida contains three families: Argasidae (soft ticks having dorsum without chitin), Ixodidae (hard ticks having dorsum totally or partially covered with chitin) and Nuttalliellidae (an ill known monotypic family represented by Nuttalliella namaqua), among which Argasidae and Ixodidae are more important. In turn, according to morphological characters, the family Ixodidae is subdivided into the Prostriata group (genus Ixodes) and Metastriata group (all other genera in Ixodidae).

Traditionally, classifications and phylogenetics inferences for Ixodida were based on morphological, biological and ecological characteristics [16–21]. Tick classification largely based on morphological characteristics, and the value given to differences and similarities among groups of ticks, resulting in non-homogeneous tick arrangements. The molecular taxonomy associated with conventional morphological cataloging will be useful to obtain a more homogeneous and independent criterion for classification, although in the short term this may not be obvious.

#### **1.1 Importance of tick identification in dogs**

Many ticks are responsible for causing various diseases. Among which the tick *Rhipicephalus sanguineus* (brown dog tick) plays vital role because it causes ehrlichiosis in dogs which is life threatening disease in dogs which causes symptoms similar to that of dengue in human beings. Reduction in platelet count and multi-organ failure are the major detrimental things in case of dogs in this specific ehrlichiosis disease. That is why identification of ticks is very important to rule out the disease and early identification will help in saving the life of the animal by giving appropriate treatment. Presence of ticks itself will help in identifying subacute cases so that life loss can be avoided. Due to its veterinary and public health relevance, *Rhipicephalus sanguineus* is one of the most studied ticks.

Ticks able to survive in adverse conditions too as they have heavy protective, chitinous covering and can withstand long periods of starvation and also have wide host range. They can deposit large number of eggs at a time and are relatively free from natural enemies and are tenacious blood suckers.

Medical and veterinary importance of ticks based on their capability of disease transmission. The important diseases transmitted by ticks are Lyme borreliosis (*Borrelia burgdorferi*), *Canine babesiosis* (*babesia* sp.), Ehrlichiosis (*Ehrlichia* sp.), Anaplasmosis (*Anaplasma* sp.), Hepatozoonosis (*Hepatozoon* sp.).

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**Figure 1.**

Rhipicephalus sanguineus *ticks.*

*Identification of Ticks in Dogs with Ehrlichiosis DOI: http://dx.doi.org/10.5772/intechopen.92244*

The dogs presented to Teaching Veterinary Clinical Campus that were diagnosed for ehrlichiosis by nPCR were utilized for the study. Around 3 or 4 ticks collected from different sites of the affected dogs were fixed in a 70% ethanol solution. It was further processed and was identified as per the morphology described by [14]

**2. Materials and methods**

*Identification of Ticks in Dogs with Ehrlichiosis DOI: http://dx.doi.org/10.5772/intechopen.92244*
