*3.2.1 CMV molecular diagnostics*

The local isolate mentioned in paragraph 3.1 obtained by Prof. Dr. Maadh AbdulWahab Al-Fahad was molecularly diagnosed by means of the technique of polymerase chain reaction, RT-PCR and according to the following steps, which were obtained from the producing company (BiONEER) for the kit (Diagnostic kit) with some modifications which included:

	- A. SB solution with a strength of 1X.
	- B. B-Loading buffer 10x magnification.

Preparation of the Agarose Gel and Electrophoresis Procedure:
