**3.5 Determination of active compounds in the dry leaves of medicinal plant extracts**

The active compounds were diagnosed in the dry leaves of Sispan, Albizia and licorice roots by using the High Performance Liquid Chromatography (HPLC) device. The dry leaves of Albizia, Sisban and licorice roots were extracted according to the method [100, 101]. The concentration of the active compounds was measured using the following model concentration equation:

$$\text{Sample concentration } \text{mg/ml} = \frac{\text{Space package form}}{\text{Area of the standard solution}} \\ \times \text{Standard solution concentration} \\ \times \text{Number of vibrations} \tag{1}$$
