*2.3.4.2* Albizia spp*.*

Albizia is widely spread in the world and the scientific name of Albizia is (L) Albizzia lebbeck and belongs to the family of Leguminosae. Its leaves are large and a fragrant group of yellow flowers and long seeds. It belongs to the family Leguminosae and is found in tropical Asia and is widely cultivated in other tropical and subtropical regions including Malawi [84]. Its flowers, bark, roots and stems are all used in medicine [85].

Albizia is an important plant as an important source of pesticides and because it contains important chemical compounds such as Saponins Alkaloids, Flavonids, Tannius [86]. The common name is bacha chin, ficus and acacia, and it is widely cultivated in the regions of tropical Asia, Africa and subtropical Asia [87].

It has many uses, including medicinal, environmental and economical, and is used as shade trees as well as wood production, and is considered to be of natural importance [88]. Its seeds contain lectins that are toxic to humans and animals.

#### *2.3.4.3* Glycyrrhiza spp*.*

The (Licorice) *Glycyrrhiza glabral,* which belongs to the Leguminosae family, and one of the most famous genera is the Glycyrrhizin plant, which contains 20 species. Glycyrrhiza, which means sweet veins in Greek.

Licorice contains many chemical compounds, as it is characterized by the availability of chemical compounds that have a sweet taste, and it contains Glycyrrheiel, Liguoric acid and flavonoids, including Glabridin and Glabrin [77]. Glycyrrhizin with its acid is the most important ingredient, it has an activity similar to that of steroid hormones, i.e. plant hormones are formed and thus lead to an increase in protein formation and an improvement in the indicators of vegetative growth [89].

The plant spreads in Asia Minor and Turkmenistan in the east to Spain in the west, which are its original home and also because of its medicinal value It has become cultivated on large areas in France, Spain, Germany, Russia, Italy, China, Turkey and America as well as the Arab Gulf and Iraq [90].

Al-Ajili [91] emphasized that licorice has high concentrations of amino acids as well as carbohydrates and other important elements, which leads to its great importance in nutritional uses. Licorice, G. glabral, a type of the Leguminosae family, is of great medical importance, as it is used for treating stomach irritation, coughing, hoarseness, bladder and kidney inflammation, as well as gout and muscular rheumatism, the autumn season are among the best dates for collecting licorice, and the reason is because the active substances are more abundant in this season, and Autumn is the best time to collect licorice because the active ingredients are more abundant [92].

Chemical analysis of licorice extract revealed that there are phenolic compounds, terpenes, gummies, starch, and flavonids extracts due to phenolic compounds for resistance to the microbes *Candida albicans*, *Staphylococcus aureus*, and Mycobacterium semegmutis, and the effect of extracts of ten species, including licorice, which showed very high efficacy in inhibiting TYLCV, reaching 100% for all concentrations used [93].

Licorice leaves are used to extract antimicrobial agents such as Pincembrin and Licoflavanone [94]. And the extract is used to protect against atomic radiation, as there is in the extract the substance alpha-amyrin that combines with the substance Methyluracil to form a compound that works to protect against atomic radiation, Maliuta [95]. And the World Health Organization (WHO) stated that licorice extract treats laryngitis and is used as an expectorant to treat colds and coughs, as well as bronchitis, to treat rheumatism, to protect the liver from poisoning and to arthritis, and to treat tuberculosis [96]. The active ingredients are Glycyrrhizin, Flavon Glycosides, Liquiritoside,Isoliquiritoside, Sucrose, Dextrose, Starch, Proteins, Liposomes, Resin, Asparagine, Sterilic Oil, Saponins [97].

spirulina, crushed and placed in 54 ml of distilled water, each 1 g, 9 ml of distilled

*The Activity of New Bio-Agent to Control* Cucumovirus Cucumber Mosaic Virus *(CMV)*

An isolate of *Pseudomonas florescence* (pf) was obtained from the biocide Bactvipe provided by International Panacea Ltd. Sterilized and ready, the hood was sterilized and the medium was incubated in the incubator for a period of (72 hours) at a temperature (38° C). It was observed that the growth rate of bacterial colonies

The dilution was used (10–4.10-5.10-6.10-7) and the fastest growing dilution and the best was the 6–10 dilution of the biological product where the plate was taken and added to a baker containing 500 ml distilled water to soak the seeds in it

A water-based extraction method was used to obtain the active compounds in

Amino acids were estimated based on the method presented by Scriver et al. [99]

**Time Methanol A Buffer B Flow rate** 20 80 1 ml/min –13 40 60 1 ml/min –25 90 10 1 ml/min

**3.5 Determination of active compounds in the dry leaves of medicinal plant**

The active compounds were diagnosed in the dry leaves of Sispan, Albizia and licorice roots by using the High Performance Liquid Chromatography (HPLC) device. The dry leaves of Albizia, Sisban and licorice roots were extracted according to the method [100, 101]. The concentration of the active compounds was mea-

> Area of the standard solution � Standard solution concentration

� Number of dilutions (1)

the licorice plant, and this method was done according to Al-Ajili [91].

water was placed [75].

reached 100% of the dishes used in agriculture.

*DOI: http://dx.doi.org/10.5772/intechopen.96587*

*3.3.3 Extracts (sespan, albizia, and licorice)*

The method mentioned by Qasim and Ali [98].

**3.4 Determination of amino acids of plant extracts**

and according to the conditions attached in table below:

sured using the following model concentration equation:

Sample concentration mg*=*ml <sup>¼</sup> Space package form

*3.3.2 PF bacteria*

for 2 hours.

*3.3.4 CMV virus extract*

**extracts**

**157**
