**2.5 Bio-distribution of doxorubicin**

CPX1 and CPX2 solutions and free DOX solution were separately injected into liver tumor bearing mice via tail vain at a dose of 20 mg/kg body weight. Different organs were harvested from the experimental mice bearing implanted tumors. Doxorubicin in the organ was extracted according a reported method and quantified by the examination of its fluorescence intensity at 590 nm (Bigotte, 1985).
