**8. References**

166 Liver Tumors

Fig. 11. Immunohistochemical pathological study. Positive membrane staining for stem cell markers CD166, 35264, CD166 LYO 1 mL *EpCAMhigh-*CD166+ in most cells of metastatic

Borrego et al. (2010) analyzed, as did Kokudo et al. (2002), p53 expression. The immunohistochemical markers tested in their study (p53, Ki-67) were not poor prognostic factors, in agreement with Saw et al. (2002). By contrast, authors such as Tanaka et al. 2004 reported that p53 or Ki-67 expression had a negative impact on survival. It should be noticed, however, that in Borrego-Estella et al.'s study survival was longer than 5 years in patients with high Ki-67 levels and in those with a high mitotic index (>10 mitosis/mm2), which is also another expression of the tumour proliferation index. However, no significant relationship was found between cell proliferation, as measured by Ki-67 and p53, whose

For O'Brien et al*.* (2007), most CD133+ stem cells had a 200-fold greater oncogenic potential than CD133- cells for development of CRC. In addition, this subpopulation is able to maintain itself and to differentiate and become established again as a tumour when transplanted in certain solid organs of experimental animals. For Borrego-Estella et al. significant trends were found in their series with regard to membrane CD133 and CD166 markers. According to O'Brien et al*.* and Ricci-Vitiani et al*.* 2007, in several CRC, CD44 was more determinant than CD133, because CD44 was expressed in tumour lines not expressing

To compare the results of Borrego et al. with other groups is difficult since many authors (Fong et al. 1999; Pawlik et al. 2005) did not performed immunohistochemical studies. However, regarding immunohistochemical markers, more significant results were not achieved probably because the only technique performed was immunohistochemistry array,

Another interesting marker, microsatellite instability, is a measure of the inability of the DNA mismatch repair system to correct errors that occur during DNA replication. It is the alternative pathway to chromosomal instability with loss of heterozygosity in colorectal

adenocarcinoma. Menarini Diagnostics® (x40).

changes express a loss of cell cycle control, and survival.

but not flow cytometry or other molecular biology techniques.

CD133.


Azoulay D., Eshkenazy R., Andreani P. Castaing D., Adam R., Ichai P., Naili S., Vinet E.,

Battifora H. (1986) The multitumour (sausage) tissue block: novel method for

Belli G., D'Agostino A., Ciciliano F., Fantini C., Russolillo N., Belli A . (2002) Liver resection

Benoist S., Brouquet A., Penna C., Julié C., El Hajjam M., Chagnon S., Mitry E., Rougier P.,

Bipat S., van Leeuwen MS., Comans EF., Pijl ME., Bossuyt PM., Zwinderman AH., Stoker J.

Bipat S., van Leeuwen MS., IJzermans JNM., Comans EFI., Planting AST., Bossuyt PMM.,

Bismuth, H. (1982) Surgical anatomy and anatomical surgery of the liver. *World J Surg* 6: 3-9,

Bluemke DA., Paulson EK., Choti MA., DeSena S., Clavien PA. (2000) Detection of Hepatic

Bonnet D., Dick J. (1997) Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell. *Nature Medicine* 3:730-737 Borrego V., Serrablo A., Hörndler C. (2010). Study of liver metastases of colorectal cancer

Casanova D., Figueras J., Pardo F. (2004) Metástasis hepáticas. In: Guía Clínica de la Asociación Española de Cirugía: Cirugía hepática. Arán, Madrid; pp 164-76. Cellini C., Hunt SR., Fleshman JW., Birnbaum EH., Bierhals AJ., Mutch MG. (2010) Stage IV

Chami L., Lassau N., Malka D., Ducreux M., Bidault S., Roche A., Elias D. (2008). Benefits of

Charnsangavej C, Clary B, Fong Y., Grothey A., Pawlik TM., Choti MA. (2006). Selection of

Choti M., Sitzmann J., Tiburi ME., Sumetchotimetha W., Rangsin R., Schulick RD., Lillemoe

Coenegrachts K., De Geeter F., ter Beek L., Walgraeve N., Bipat S., Stoker J., Rigauts H.

resection for hepatic colorectal metastases. *Ann Surg* 235: 759-66.

Couinaud C (1957). Le Foie, Études Anatomiques et Chirurgicales. Masson

chemotherapy: does it mean cure? J Clin Oncol 20;24(24):3939-45

liver metastases in the Netherlands. *Ned J Med* 63(1): 1-14

Imaging and Dual-Phase Helical CT. *AJR* 175: 1653–1658

immunochemical antibody testing. *Lab Invest* 55:244-8

*Surg* 241: 277-285

ISSN 0364-2313

4.

379

analysis. *Radiology* 237: 123-31

tumour? *World J Surg* 34(5): 110

*Ann Surg Oncol* 13 (10): 1261-1268.

with Histologic Findings. *AJR* 190: 683–690

– 613

Saliba F., Lemoine A., Gillon MC., Bismuth H. (2005). In situ hypothermic perfusion of the liver versus standard total vascular exclusion of complex liver resection. *Ann* 

for hepatic metastases: 15 years of experience. *J Hepatobiliary Pancreat Surg* 9 (5): 607

Nordlinger B. (2006) Complete response of colorectal liver metastases after

(2005). Colorectal liver metastases: CT, MR imaging, and PET for diagnosis-meta-

Greve J-W., Stoker J. (2007) Evidence-based guideline on management of colorectal

Lesions in Candidates for Surgery: Comparison of Ferumoxides-Enhanced MR

with surgical rescue. Identification of prognostic biological markers. University of Salamanca Editions. Collection VITOR. 1st Ed. pp. 243-248. ISBN: 978-84-7800-205-

rectal cancer with liver metastases: is there a benefit to resection of the primary

Contrast-Enhanced Sonography for the Detection of Liver Lesions: Comparison

patients for resection of hepatic colorectal metastases: expert consensus statement.

KD., Yeo CJ., Cameron JL. (2002). Trends in long term survival following liver

(2009). Comparison of MRI (including SS SE-EPI and SPIO-enhanced MRI) and FDG-PET/CT for the detection of colorectal liver metastases. *Eur Radiol* 19(2): 370-


Garden OJ., Rees M., Poston GJ., Mirza D., Saunders M., Ledermann J., Primrose JN., Parks

Guglielmi A., Pachera S., Ruzzenente A. Surgical Therapy of Hepatic Metastases. In: Delaini

Harmantas A., Rotstein LE., Langer B. (1996) Regional versus systemic chemotherapy in the

Hemming AW., Chari RS., Cattral MS. (2000). Ex vivo resection. *Can J Surg* 43(3): 222-224 Hemming AW., Reed AI., Langham MR Jr., Fujita S., Howard RJ. (2004) Combined

Hill RP. (2006) Identifying cancer stem cells in solid tumours: case not proven. *Cancer Res* 66:

Hoti E., Salloum C., Azoulay D. (2011) Hepatic resection with in situ hypothermic perfusion

Ieta K., Tanaka F., Haraguchi N., Kita Y., Sakashita H., Mimori K. (2008) Biological and

Ishiguro S., Akasu T., Fujimoto Y., Yamamoto J., Sakamoto Y., Sano T., Shimada K., Kosuge

Jaeck D. (2003) The significance of hepatic pedicle lymph nodes metastases in surgical

Jaeck D., Oussoultzoglou E., Rosso E., Greget M., Weber JC., Bachellier P. (2004) Two-stage

Jordan CT., Guzman ML., Noble M. (2006) Cancer stem cells. *N Engl J Med* 355: 1253-1261. Karoui M., Penna C., Amin-Hashem M., Mitry E., Benoist S., Franc B., Rougier P.,

Kinkel K., Lu Y., Both M., Warren RS., Thoeni RF. (2002) Detection of hepatic metastases

Koh DM., Scurr E., Collins DJ., Pirgon A., Kanber B., Karanjia N., Brown G., Leach MO.,

Kokudo N., Miki Y., Sugai S., Yanagisawa A., Kato Y., Sakamoto Y., Yamamoto J.,

hepatectomy for colorectal liver metastases. *Ann Surg* 243: 1-7.

focal hepatic lesions. *World J Gastroenterol* 14; 15(46): 5805-5812

(US, CT, MR Imaging, PET): a meta-analysis. *Radiology* 224: 748-756

is superior to other resection techniques. *Dig Sur*; 28(2): 94-9

(Suppl 3): iii1–iii8

8859174.

239: 712–721

1891-1895.

638-648.

*Oncol* 13: 1579 – 87

*Surg Oncol* 10: 1007–1011

metastases. *Ann Surg* 240: 1037-1049.

Springer-Verlag Milan 2005

RW. (2006). Guidelines for resection of colorectal cancer liver metastases*. Gut* 55

GG. Rectal Cancer: New Frontiers in Diagnosis, Treatment and Rehabilitation. ©

treatment of colorectal carcinoma metastatic to the liver. Is there a survival difference? Metanalysis of the published literature. *Cancer* 78: 1639-45. PMID:

Resection of the Liver and Inferior Vena Cava for Hepatic Malignancy. *Ann Surg*

genetic characteristics of tumour initiating cells in colon cancer. *Ann Sur Oncol* 15:

T., Yamamoto S., Fujita S., Moriya Y. (2006) Second hepatectomy for recurrent colorectal liver metastasis: analysis of preoperative prognostic factors. *Ann Surg* 

management of colorectal liver metastases and of other liver malignancies. *Ann* 

hepatectomy procedure combined with portal vein embolization to achieve curative resection for initially unresectable multiple and bilobar colorectal liver

Nordlinger B. (2006) Influence of preoperative chemotherapy on the risk of major

from cancers of the gastrointestinal tract by using noninvasive imaging methods

Husband JE. (2006) Colorectal hepatic metastases: quantitative measurements using single-shot echo-planar diffusion-weighted MR imaging. *Eur Radiol* 16: 1898–1905 Koike N., Cho A., Nasu K., Seto K., Nagaya S., Ohshima Y., Ohkohchi N. (2009) Role of

diffusion-weighted magnetic resonance imaging in the differential diagnosis of

Yamaguchi T., Muto T., Makuuchi M. (2002). Genetic and histological assessment

of surgical margins in resected liver metastases from colorectal carcinoma: minimum surgical margins for successful resection. *Arch Surg* 137: 833-40


Kupffer-phase image obtained by sonazoid in patients with colorectal liver metastases. *World J Gastroenterol* 28; 14(20): 3207-3211


Narita M., Oussoultzoglou E., Jaeck D., Fuchschuber P., Rosso E., Pessaux P., Marzano E.,

Niekel MC., Bipat Sh., Stoker J. (2010) Diagnostic Imaging of Colorectal Liver Metastases

Nishio H., Hamady ZZ., Malik HZ., Fenwick S., Rajendra Prasad K., Toogood GJ., Lodge JP.

Nomura K., Kadoya M., Ueda K., Fujinaga Y., Miwa S., Miyagawa S. (2007) Detection of

Nordlinger B., Vaillant JC., Guiguet M., Balladur P., Paris F., Bachellier P., Jaeck D. (1994)

Nordlinger B., Guiguet M., Vaillant JC., Balladur P., Boudjema K., Bachellier P. (1996)

Nondlinger B., Sorbye H., Glimelius B., Poston GJ., Schlag PM., Rougier P., Bechstein WO.,

O'Brien CA., Pollett A., Gallinger S., Dick JE. (2007) Chemotherapy and cancer stem cells.

Ohlsson B., Stenram U., Tranberg KG. (1998) Resection of colorectal liver metastases: 25 year

Oldenburg A., Albrecht T. (2008) Baseline and contrast-enhanced ultrasound of the liver in

Oldhafer KJ., Lang H., Malago M., Testa G., Broelsch CE. (2001) Ex-situ resection and

Patel S., McCall M., Ohinmaa A., Bigam D., Dryden DM. (2011) Positron emission

Pawlik T., Assumpcao L., Vossen J., Buijs M., Gleisner A., Schulick R., Choti M. (2009)

Pawlik TM., Schulik RD., Choti MA. (2008) Expanding Criteria for Resectability of

Petrowsky H., Gonen M., Jarnagin W., Lorenz M., DeMatteo R., Heinrich S., Encke A.,

for hepatic colorectal metastases. *Ann Surg Oncol* 16(2): 371-378

Colorectal Liver Metastases. *Oncologist* 13(1): 51-64

resection of the in-situ perfused liver: are there still indications? *Chirurg* 72(2): 131-

tomography/computed tomographic scans compared to computed tomographic scans for detecting colorectal liver metastases: a systematic review. *Ann Surg* 253(4):

Trends in nontherapeutic laparotomy rates in patients undergoing surgical therapy

Blumgart L., Fong Y. (2002) Second liver resections are safe and effective treatment

cases. Association Francaise de Chirurgie. *J Clin Oncol* 12 :1491-6

randomised controlled trial. *Lancet* 371: 1007-16

tumour patients. *Ultraschall Med* 29(5): 488-98. Abstract

experience. *World J Surg* 22: 268-76

metastases. *World J Gastroenterol* 28; 14(20): 3207-3211

metastases. *Br J Surg* 98(10): 1463-75

Treatment. *Radiology* 257(3): 674-684

*Surg Onco*l 33: 729–34

*Nature* 445: 106-10

7. Abstract

666-71

*Clin Gastroenterol* 41(8)789-95

Kupffer-phase image obtained by sonazoid in patients with colorectal liver

Bachellier P. (2011) Two-stage hepatectomy for multiple bilobar colorectal liver

with CT, MR Imaging, FDG PET, and/or FDG PET/CT: A Meta-Analysis of Prospective Studies Including Patients Who Have Not Previously Undergone

(2007) Outcome following repeat liver resection for colorectal liver metastases. *Eur J* 

Hepatic Metastases From Colorectal Carcinoma: Comparison of Histopathologic Features of Anatomically Resected Liver With Results of Pre-operative Imaging. *J* 

Survival benefit of repeat liver resections for recurrent colorectal metastases: 143

Surgical resection of colorectal carcinoma metastases to the liver. A prognostic scoring system to improve case selection, based on 1568 patients. *Cancer* 77: 1254-62

Primrose JN., Walpole ET., Finch-Jones M., Jaeck D., Mirza D., Parks RW., Collette L., Praet M., Bethe U., Van Cutsem E., Scheithauer W., Gruenberger T. (2008) Perioperative chemotherapy with FOLFOX4 and surgery versus surgery alone for resectable liver metastases from colorectal cancer (EORTC Intergroup trial 40983): a for recurrent hepatic metastases from colorectal cancer: a bi - institutional analysis. *Ann Surg* 235: 863–71

