*6.2.1 Biofertilizer (*X. autotrophicus*) for bioremediation of environment polluted by chemical agents*

In that sense EPGPB (likes PGPM or/and PGPR can be classified as biofertilizers when they sustainable options to plant nourishment and enrichment source that would useful for bioremediation and/or phytoremediation (double actions plants and biofertilizer) for soil contaminated by chemical agents [87, 88]. There for *X. autotrophicus* is has been applied in bioaugmentation trials for cleaning up any environmental impacted by chemical agents [86] which due to powerful genetic capacity is able to degradate a wide range of chemical agents under several environmental conditions either soil and or water in that sense it been reported that *X. autotrophicus* is able to biodegradate of 1,2- dichloroethane (DCE) one of the largest Xanthobacter autotrophicus *an Endophytic Beneficial Bacterium for Wheat and Other Plants… DOI: http://dx.doi.org/10.5772/intechopen.102066*

chlorinated industrial chemical, most of it being used for synthesis of vinyl chloride and smaller amounts for ethylene diamine and other chemicals. It was also used as a solvent. Groundwater contamination is mainly due to leakages and improper waste disposal. *X. autotrophicus* can attack DCE by using some specific enzimes under oxic conditions was investigated in the 1980s [89, 90] required for prolonged groundwater bioremediation polluted by DCE. Such systems are operated under non-sterile conditions, and long-term survival of enzymes would require separate enzyme production and a process allowing for physical separation of the biocatalyst from groundwater. There may be attractive application opportunities if biotransformation of synthetic chemicals in waste streams leads to products that can be recycled, e.g., when a wastewater product can cleaning up. This issue that received attention during the development of strains of *X. autotrophicus* growing on 1,2,3-trichloropropane (TCP) and another xenobiotic compound that polluted wastewater [86, 90–92].

A bioformulation is not effective until it does not have an impact in field conditions, market existence and reliability and cost effectiveness [93]. Production of bioformulation is not only dependent on the detailed knowledge of microbial as well as plant physiology, but a number of technological challenges are also involved such as fermentation process, formulation type, population of microbe, and delivery systems [94]. Barea [59] has published that in order to get better bioformulation for any domestic crops is important to understand the interaction among EPGPB or PGPM. To reproduce those microorganisms is important the chemical composition of broth media as well as the main and best conditions for each microorganisms need to get enough amount of them for bioformulation applying in open agriculture [95]. Including legal and ecological permission for safe crops production. A key quality of any bioformulation has to be water soluble to make sure a positive effect on any domestic crop Himel et al. [96] and Bateman [97] underline for those bioformulation which are applying in in aerosol based on a droplet size that is sufficient to inoculate seeds and plants with excellent results. For bioformulations applied foliarly, it is important to consider all environmental factors: solar radiation, high temperatures, ultraviolet light, etc. that limit the survival of beneficial plant microorganisms [98]. In this sense, the type of bioformulations must be appropriate to the form and vehicle that transport the beneficial plant microorganisms according to the recommended application directly to the soil, to the seeds or plants so that the forecast of the result favors agricultural production or control of some disease or pest [99]. Therefore, it is important research for the innovation of bioformulation suitable for agricultural crops [58] that comply with the quality and legality standards to satisfy the world market demand for safe food without risk of environmental damage [100]. A fundamental aspect for the world market of biological inoculants has been the necessary implementation of microbiological quality controls with reliable protocols that are endorsed by laws in the world that protect those farmers who, when applying them, have the confidence that they will have positive results in production. agriculture, due in part to the unfortunate experience of bioformulations without microbiological or legal quality that have caused a rejection of some sectors involved in sustainable agricultural production, an aspect that has not yet been resolved in the world [68]. In an integral sense that the biotechnology of the formulation of inoculants requires solid research for the best selection of microorganisms that promote plant growth, as well as the protocols of legal and ethical microbiological quality in the generation of bioformulations that give confidence to be used in the world for a sustainable and harmless agricultural production in harmony with the environment [85].
