**1. Introduction**

*Pseudomonas aeruginosa* is an opportunistic Gram-negative bacilli bacterium which holds a greater clinical significance in relation to its infection causing ability in humans [1]. *P. aeruginosa* is commonly found in environment (soil and water) and can be a source of contamination of drinking water and food spoilage [2, 3]. Prevalence of *P. aeruginosa* and its associated infection is commonly found in cystic fibrosis patient and chronic obstructive pulmonary disease (COPD) lungs, urinary tract, immunocompromised (HIV) patients, skin and soft-tissue, diabetic leg wounds, burns and surgical site infections [1, 4]. It is also a common pathogen responsible for causing healthcare associated (nosocomial) infection and microbial keratitis (eye infection due to contamination of contact lenses) [4]. World Health Organization (WHO) has listed *P. aeruginosa* as a most critical pathogen, due to the threat of causing blood stream infection (septicemia) and its antibiotic resistance ability [5]. *P. aeruginosa* or in general many other bacterial pathogens (e.g. *Acinetobacter baumannii*, *Escherichia coli*, *Klebsiella pneumoniae*, *Staphylococcus aureus*, *Streptococcus epidermidis*,

*Streptococcus pyogenes*, *Proteus mirabilis*, etc.) has an inheritance ability to colonize and form biofilms on biotic (e.g. mucosa, tissue) or abiotic surface (e.g. medical implants, surgical instruments, hospital beds, wash basins, sinks, bath tub, etc.). Bacterial colonization on these surfaces directly leads to the contamination of surfaces, food and water and consequently precedes to infections in host. Bacterial biofilms are liable for approximately 80% of hospital and community-associated infections [6]. The most serious concern is antibiotic/antibacterial agents' resistance by the infecting bacteria that threatens the very core of modern medicine and impose a greater burden on global public health and economy.
