Meet the editors

Dr. Hirobumi Suzuki received his Ph.D. in 1997 from Tokyo Metropolitan University, Japan, where he studied firefly phylogeny and the evolution of mating systems. He is especially interested in the genetic differentiation pattern and speciation process that correlate to the flashing pattern and mating behavior of some fireflies in Japan. He then worked for Olympus Corporation, a Japanese manufacturer of optics and imaging products, where he

was involved in the development of luminescence technology and produced a bioluminescence microscope that is currently being used for gene expression analysis in chronobiology, neurobiology, and developmental biology. Dr. Suzuki currently serves as a visiting researcher at Kogakuin University, Japan, and also a vice president of the Japan Firefly Society.

Dr. Katsunori Ogoh received his Ph.D. from Shizuoka University, Japan, in 2005 for his study on sea-firefly. He is a member of the Research and Applications team of Olympus, specializing in biological techniques, and works in the sales and marketing department of microscopes, conducting research and development on the use of advanced analytical systems and applications, including cell culture, gene cloning, and microscopy applica-

tions. Dr. Ogoh has a powerful motivation for research and is working on bioluminescence technology development with his positive thinking and tireless efforts. He is inspired by diving in the sea. In his free time, he likes to go camping and make DIYs, and plays Splatoon video game with his family.

Contents

**Section 1**

**Section 2**

Evaluation in Thailand *by Anchana Thancharoen*

**Preface XI**

Bioluminescence Techniques **1**

**Chapter 1 3**

**Chapter 2 19**

**Chapter 3 33**

**Chapter 4 47**

Bioluminescent Organisms **57**

**Chapter 5 59**

Fluorescent Markers: Proteins and Nanocrystals

*Eurípedes Alves da Silva Filho, Marcelo Duzzioni, Edigar Henrique Vaz Dias, Fábio de Oliveira, Juliana Rodrigues Machado, Malu Mateus Santos, Marcos Vinícius da Silva, Carlo José Freire de Oliveira,* 

*Virmondes Rodrigues Junior, Lucas Anhezini* 

Reaction of Human Serum Albumin

Dual-Color Bioluminescence Microscopy

*and Noelio Oliveira Dantas*

*by Anielle Christine Almeida Silva, Jerusa Maria de Oliveira, Kelen Talita Romão da Silva, Francisco Rubens Alves dos Santos, João Paulo Santos de Carvalho, Rose Kethelyn Souza Avelino,* 

Design of Coelenterazine Analogue to Reveal Bioluminescent

Near-Infrared Luciferin Analogs for *In Vivo* Optical Imaging *by Ryohei Saito-Moriya, Rika Obata and Shojiro A. Maki*

Imaging Promoter Assay of Adenylyl Cyclase A Gene in *Dictyostelium discoideum* during Fruiting Body Formation by

*by Taro Hayashi, Katsunori Ogoh and Hirobumi Suzuki*

Firefly Translocation: A Case Study of Genetic and Behavioral

*by Ryo Nishihara, Kazuki Niwa, Tatsunosuke Tomita and Ryoji Kurita*
