*2.4.3.1 Tail immersion test*

This test used by Ramabadran et al. [18] assesses the basic pain response in mice to thermal stimuli. This test measures spinally driven aspects of pain. Here, the

animals were immobilised using tube restrainer (which also allowed free movement of the tail). The distal half of each mouse tail was immersed in water bath (at 50°C) contained in a beaker with 20 s time-out. The duration of time taken for the mice to flick its tail away from the heat of the hot water was measured in seconds. This is known as latency of flick [19].
