**2.2 Inactivation of** *Candida albicans*

The commonest methods for inactivation of *C. albicans*, in the preparation of an immune-based prevention of *C. albicans* infection include using of heat and a source of UV. According to Evron, [26], whole *C. albican* cells suspended in 0.85% sterile normal saline and heated at 65°C are inactive. While exposing *C. albicans* suspended in 0.85% sterile normal saline directly to a source of Ultraviolet radiation (UV) at a wavelength of 254 nm for 30 minutes inactivates the cells [27]. This inactivation renders the *C. albicans* non-viable to infect an intended host but retains the structural conformation of immunogenic components on the cell surface.
