Filling the gap of the model.
• Search the published literature first on metabolome of C. albicans
• Give the second priority to genome annotation tools (KEGG - https://www.kegg.jp/kegg-bin/show_
  organism?menu_type=pathway_maps&org=cal) and organism specific database (Candida genome
  database - http://www.candidagenome.org/).
• Last precedence gives to closest organism database (Saccharomyces - https://www.yeastgenome.
  org/)
• Document all the gap fill reactions with references.
• Add gap fill reaction by repeating the stage 2 till the biomass production occurs. For addition or
  deletion use the command given below-
> > model_add = addReaction(model, 'ReactionName', 'Reaction');
> > model_del = removeRxns(model, 'ReactionName', 'Reaction');
• After filling the gaps, check the production of biomass of the cell as commands given below:
> > initCobraToolbox;
> > solverok = changeCobraSolver('glpk','LP');
> > model = xls2model(CanCyc.xlsx');
> > model = changeObjective(model,'BiomassReac_1');
> > FBAsolution = optimizeCbModel(model,'max')