**11. Conclusion**

This review focuses on the functions of sRNA and their role in regulating genes in *S. aureus*. Combined application of High throughput screening (HTS), genomic analysis and phenotypic methods for the prediction and determination of sRNAs, functional proteins, RNA binding proteins and riboswitches would provide information on the mechanism of integration of proteins and regulatory RNAs into interwined regulatory networks responsible for adaptation to stress conditions and virulence production [100]. Further study is needed for the determination of signals that can initiate the regulation of sRNA transcription and their targets. Another point that needs to be focused on is host-virulence adaptation or interactions, then cell communication among the dense population of microorganisms and cell differentiation. The expression pattern of sRNAs will be different in a population, leading to adaptability in response to various environmental and stress variations. Furthermore, variations in sRNA expression and their regulatory networks

due to host-microbiome interactions also need to be studied. Metagenomic studies, HTS approach, RNA-seq analysis and transcriptomics studies could help understand the mechanisms by which the commensal pathogens cause infections and disease.
