**4. Biochemical tests for differentiating** *Staphylococcus aureus* **and Staphylococcus intermedius**

A rapid test for the detection of acetoin has been developed [3]. Purple agar, containing bromocresol purple as a pH indicator and 1% maltose, is used to differentiate S. aureus from S. intermedius [4]. Purple is the color of most of the colonies of that bacteria. The energy source used by the *Staphylococcus aureus* in the culture medium is maltose which is utilized by that microbe and the resultant metabolic byproduct is acid production. The by-product acid changes the color of the medium and colonies to yellow. Staphylococcus intermedius is a maltose fermenter so it means that it will not affect the color of the medium. There is also the commercial availability of the Biochemical tests which can be used for the confirmation of the staphylococcal species which can further be confirmed by molecular techniques like a polymerase chain reaction and multiplex PCR [5]. There are also studies on the molecular typing of the isolates of different regions of the world. The techniques that are and can be used in near future for the molecular epidemiology of the different isolates of Staphylococcus species can be but not limited to the Multilocus Sequence Typing (MLST) [6–9] and Multilocus variable number of Tandem Repeats (MLV) [10–12].
