**Figure 4.**

*Immunofluorescent staining of natural killer T cells in the intestinal allograft. Immunostaining of an intestinal allograft. Green signal, FITC and red signal, phycoerythrin [PE]. Nuclei are stained with DAPI (blue). Brown signal was visualized with DAB. (A) TCRV*α*24 (200×) and (B) TCR*β*11 (200×). (C, D) TCRV*α*24 (green) and IL-4 (red) (IL-4 positive iNKT is indicated by an arrow). The observation magnification is 200× in both cases. (E, F) TCRV*α*24 (red) and TUNEL (green). (E) TUNEL+ (apoptotic) TCRV*α*24 + iNKT cells are observed at the onset of ACR (100×) and (F) 48 h after the onset of ACR (100×). Doubly stained cells were increased 48 h after the onset of ACR. (G) CD1d+ dendritic cells. CD1d and CD11c were stained green and red, respectively. (H) TCRV*α*24 stained iNKT cells (red) and CD1d stained dendritic cells (green). (I) FasL+ (green) TCRV*α*24+ (red) iNKT cells. The observation magnification is 400 x in (G)-(I)."*

detecting the immunological activation with the appearance of FasL-positive T/ NKT cells and apoptotic bodies in the lamina propria. The treatment substantially prevented the progression of the crypt apoptosis [17, 18, 21, 22].
