**7. Culture and sensitivity tests**

**Rationale:** The idea of culturing microorganisms is not new or foreign to the practice of dentistry. There are situations in dentistry where it is not only important to know whether microorganisms are present in a lesion but also the type of microorganism. It follows from this that to best treat these infections the dentist needs to know what antibiotic would be most effective against the particular organism. Thus the culture and sensitivity test for bacterial organism is indispensable [7]. The biggest challenge today will be the transition from culture-based microbiological testing to molecular-based testing.

## **7.1 Collection of specimen**


#### **7.2 Tissue specimen for culture**


c.At the laboratory, using the sterile tissue grinders, the specimen is ground into suspension, which is left to settle. The supernatant fluid is then used for the inoculation of appropriate media [8].
