**Abstract**

The objective of this study was to determine the effect of tomato juice supplements consumption on the lipid profile of women with dyslipidemia patients. The design of this study was a quasi-control experimental design with pre-post test. The subjects were sixty-two Kendal Hospital women employees, aged 35–50 years old, suffering from dyslipidemia but did not suffer from metabolic or degenerative diseases based on the examination of a specialist in internal medicine. Subjects were divided into two groups, group I (30 people) were given antioxidant supplements (336 g of tomatoes per day) for 21 days and group II (32 people) as control. The nutrition intake data was measured by the 24-hour food withdrawal method conducted for three consecutive days in three weeks of research calculated by the Nutrisurvey program. Data was analyzed with Kolmogorov Smirnov test, Pearson test and Mann Whitney test. There were significant relationships between energy intake, protein intake, fat intake, and carbohydrate intake with total cholesterol levels and triglyceride levels and there were also significant relationships between energy intake and fat intake with low density lipoprotein levels. It shows that there is a relationship between diet and dyslipidemia. There were significant differences in total cholesterol, low lipoprotein levels and triglycerides in treatment and control groups. This showed that tomato juice supplements significantly affect the lipid profiles.

**Keywords:** diet, tomato juice supplements, lipid profile, cardiovascular disease

## **1. Introduction**

The relationship between blood cholesterol and heart disease has been wellestablished, with a decrease in Cholesterol Low Density Lipoprotein (LDL) being the main target of preventive therapy. High levels of LDL cholesterol in blood vessels cause these molecules to penetrate the sub-endothelial space which can be easily oxidized by free radicals. This oxidized LDL causes damage to nearby structures, causing the recruitment of monocytes to eliminate the oxidized LDL. This elimination process produces by-products in the form of foam cells. These foam cells release toxins that cause damage to endothelium cells, hypertrophy, and smooth muscle hyperplasia of blood vessels. This process also stimulates platelet

aggregation which can interfere with the production or availability of nitric oxide which results in decreased blood vessel lumen and ischemia in tissues and organs. As this inflammatory process develops, high LDL cholesterol levels can develop into atherosclerosis [1].

Cardiovascular disease (CVD) is a leading cause of human disability and premature death worldwide. Diet has a direct relationship with the development of CVD and dietary changes are the current approach CVD prevention which is to increase consumption of fruits and vegetables as a good source of some antioxidant phytochemicals, e.g. carotenoids. Lycopene is the most abundant carotenoid in tomatoes and tomato products have gained profuse attention in recent years for their beneficial of health role, especially those related to their antioxidant effects and its protective role against CVD [2]. Dietary and antioxidant supplements are efforts to prevent the development of dyslipidemia into cardiovascular disease. The 2015 US Diet Guidelines Advisory Committee summarizes the evidence for the benefits of a healthy diet on cardiometabolic outcomes and other diseases and also describes a healthy vegetarian diet, which includes more peas, soy products, nuts and seeds, and seeds. Whole grains but does not include meat, poultry, or seafood [3]. Women in the top aMed quintile have a lower risk for both Coronary Heart Disease (CHD) and stroke than bottom quintile (RR = 0.71 (95% CI = 0.62–0.82; p trend<0.0001) for CHD; RR = 0.87 (95% CI = 0.73–1.02; p trend = 0.03) for stroke). CVD mortality was significantly lower among women in the top quintile of the aMed (RR = 0.61, 95% CI = 0.49–0.76, p trend<0.0001) [4]. Lycopene may improve vascular function and contributes to the primary and secondary prevention of cardiovascular disorders. The main activity profile of lycopene includes anti-atherosclerotic, antioxidant, anti-inflammatory, antihypertensive, antiplatelet, antiapoptotic, and protective endothelial effects, the ability to improve the metabolic profile, and reduce arterial stiffness. In this context, lycopene has been shown in many studies to exert a favorable effect in patients with subclinical atherosclerosis, metabolic syndrome, hypertension, peripheral vascular disease, stroke and several other cardiovascular disorders, although the results obtained are sometimes inconsistent, which warrants further studies focusing on its bioactivity [5]. There was a study with randomized 36 statin treated CVD patients and 36 healthy volunteers in a 2:1 treatment allocation ratio to either 7 mg lycopene or placebo daily for 2 months in a double-blind trial. Post-therapy EDV responses for lycopene-treated CVD patients were similar to HVs at baseline (2% lower, 95% CI: −30% to +30%, P = 0.85), also suggesting lycopene improved endothelial function. Lycopene supplementation improves endothelial function in CVD patients on optimal secondary prevention, but not in HVs [6]. Lycopene is found in many fruits and vegetables, there is a unique situation that more than 85% of the most dominant source of lycopene in human food comes from tomatoes (*Solanum lycopersicum esculentum* Mill) and products made from tomatoes such as tomato paste, tomato sauce and tomato juice. The level of lycopene content in some tomato products varies from 8.8 μg/g in fresh tomatoes to 1200 μg/g in tomato powder. The concentration of lycopene in tomatoes varies depending on the type of tomato, season, level of maturity. The concentration of lycopene in ripe tomato juice ranges from 8600 to 9300 μg/100 g or three times higher than fresh tomatoes [7]. The study was conducted using 336 g tomato juice (350 mL) equivalent to 30 mg of lycopene with the same research time with a similar study in humans that is 3 weeks. The use of a dose of 30 mg/day based on analysis from previous studies. Studies with lycopene dose of 20 mg/day can significantly reduce total cholesterol, but not significantly in high density lipoprotein (HDL) and triglyceride (TG) [8], where studies with a lycopene dose 40 m/day show a positive correlation with total cholesterol, LDL, TG and HDL/LDL ratio, but negatively correlated with HDL [9].

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triglyceride levels.

**3. Results**

*Effect of Tomato Juice Supplements Consumption on the Lipid Profile of Dyslipidemia Patients*

This study was conducted at the Regional General Hospital Dr. H. Soewondo Kendal, because cases of dyslipidemia in employees increased significantly, in 2009 there were 67 cases and increased to 108 cases in 2012. Cases of dyslipidemia include hypercholesterolemia, hypertriglyceride, a combination of both, with Diabetes Mellitus and with other diseases. The objective of this study was to determine the effects of tomato juice supplement consumption on the lipid profile of dyslipidemic

The design of this study was a quasi-control experimental design with pre-post test. Subjects were sixty-two Kendal Hospital women employees, aged 35–50 years old, suffering from dyslipidemia but did not suffer from metabolic or degenerative diseases based on the examination of a specialist in internal medicine. Nutrition intake data was measured by 24-hour food recall method for three days in three weeks of the study. Nutritional values were analyzed using the Nutrisurvey program. Food withdrawal is carried out using a food withdrawal form through interviews conducted by nutritionists. Subjects were divided into two groups; group I as the treatment group (30 people) were given with antioxidant supplements (336 g of tomatoes per day) for 21 days and group II (32 people) as the control group. Data on total cholesterol levels, LDL cholesterol levels, HDL cholesterol levels, and triglyceride levels were measured using the chemical analysis system of vitros 300 system by clinical pathology laboratory officers of Regional Hospital Dr. H. Soewondo Kendal, accredited by the government. Measurements were taken before and after supplementation. Blood was taken at 08.00–11.00 Western Indonesian Time (WIT) and immediately checked the total cholesterol level, LDL cholesterol level, HDL

Data was analyzed with Kolmogorov Smirnov test, Pearson test and Mann Whitney test. This research has been legalized by the Health Research Ethics

with the issuance of "ETHICAL CLEARANCE" No. 329/EC/FK-RSDK/2014.

the percentage of energy intake and percentage fat intake (p < 0.05).

HDL levels and the triglyceride levels (p < 0.05).

In **Table 2** significant relationships between energy intake (P = 0.001, r = 0.501), fat intake (P = 0.001, r = 0.574), protein intake (P = 0.008, r = 335) and carbohydrate intake (P = 0.001, r = 0.446) with total cholesterol levels were observed. There was also a significant relationship between the fat intake (P = 0.012, r = 0.318) with LDL levels and there were significant relationships between energy intake (P = 0.005, r = 353), fat intake (P = 0.008, r = 336), protein intake (P = 0.016, r = 305), and carbohydrate intake (P = 0.013, r = 315) with

**Table 3** shows the lipid profile of the study subjects before the study. There were no differences between the treatment group and the control group in the total cholesterol levels and the LDL levels (p ≥ 0.05) but, there were differences in the

Commission (KEPK) Faculty of Medicine, UNDIP and RSUP DR. Kariadi Semarang,

In **Table 1**, the results showed that the profile of the study subjects did not show differences in age, BMI, cholesterol intake, Mono Unsaturated Fatty Acid (MUFA) intake, Poly Unsaturated Fatty Acid (PUFA) intake, carbohydrate intake, fiber intake, vitamin A intake, and vitamin C (p > 0.05). But there were differences in

*DOI: http://dx.doi.org/10.5772/intechopen.95239*

**2. Materials and methods**

cholesterol level, and triglyceride level.

patients.

*Effect of Tomato Juice Supplements Consumption on the Lipid Profile of Dyslipidemia Patients DOI: http://dx.doi.org/10.5772/intechopen.95239*

This study was conducted at the Regional General Hospital Dr. H. Soewondo Kendal, because cases of dyslipidemia in employees increased significantly, in 2009 there were 67 cases and increased to 108 cases in 2012. Cases of dyslipidemia include hypercholesterolemia, hypertriglyceride, a combination of both, with Diabetes Mellitus and with other diseases. The objective of this study was to determine the effects of tomato juice supplement consumption on the lipid profile of dyslipidemic patients.

#### **2. Materials and methods**

*Management of Dyslipidemia*

atherosclerosis [1].

aggregation which can interfere with the production or availability of nitric oxide which results in decreased blood vessel lumen and ischemia in tissues and organs. As this inflammatory process develops, high LDL cholesterol levels can develop into

Cardiovascular disease (CVD) is a leading cause of human disability and premature death worldwide. Diet has a direct relationship with the development of CVD and dietary changes are the current approach CVD prevention which is to increase consumption of fruits and vegetables as a good source of some antioxidant phytochemicals, e.g. carotenoids. Lycopene is the most abundant carotenoid in tomatoes and tomato products have gained profuse attention in recent years for their beneficial of health role, especially those related to their antioxidant effects and its protective role against CVD [2]. Dietary and antioxidant supplements are efforts to prevent the development of dyslipidemia into cardiovascular disease. The 2015 US Diet Guidelines Advisory Committee summarizes the evidence for the benefits of a healthy diet on cardiometabolic outcomes and other diseases and also describes a healthy vegetarian diet, which includes more peas, soy products, nuts and seeds, and seeds. Whole grains but does not include meat, poultry, or seafood [3]. Women in the top aMed quintile have a lower risk for both Coronary Heart Disease (CHD) and stroke than bottom quintile (RR = 0.71 (95% CI = 0.62–0.82; p trend<0.0001) for CHD; RR = 0.87 (95% CI = 0.73–1.02; p trend = 0.03) for stroke). CVD mortality was significantly lower among women in the top quintile of the aMed (RR = 0.61, 95% CI = 0.49–0.76, p trend<0.0001) [4]. Lycopene may improve vascular function and contributes to the primary and secondary prevention of cardiovascular disorders. The main activity profile of lycopene includes anti-atherosclerotic, antioxidant, anti-inflammatory, antihypertensive, antiplatelet, antiapoptotic, and protective endothelial effects, the ability to improve the metabolic profile, and reduce arterial stiffness. In this context, lycopene has been shown in many studies to exert a favorable effect in patients with subclinical atherosclerosis, metabolic syndrome, hypertension, peripheral vascular disease, stroke and several other cardiovascular disorders, although the results obtained are sometimes inconsistent, which warrants further studies focusing on its bioactivity [5]. There was a study with randomized 36 statin treated CVD patients and 36 healthy volunteers in a 2:1 treatment allocation ratio to either 7 mg lycopene or placebo daily for 2 months in a double-blind trial. Post-therapy EDV responses for lycopene-treated CVD patients were similar to HVs at baseline (2% lower, 95% CI: −30% to +30%, P = 0.85), also suggesting lycopene improved endothelial function. Lycopene supplementation improves endothelial function in CVD patients on optimal secondary prevention, but not in HVs [6]. Lycopene is found in many fruits and vegetables, there is a unique situation that more than 85% of the most dominant source of lycopene in human food comes from tomatoes (*Solanum lycopersicum esculentum* Mill) and products made from tomatoes such as tomato paste, tomato sauce and tomato juice. The level of lycopene content in some tomato products varies from 8.8 μg/g in fresh tomatoes to 1200 μg/g in tomato powder. The concentration of lycopene in tomatoes varies depending on the type of tomato, season, level of maturity. The concentration of lycopene in ripe tomato juice ranges from 8600 to 9300 μg/100 g or three times higher than fresh tomatoes [7]. The study was conducted using 336 g tomato juice (350 mL) equivalent to 30 mg of lycopene with the same research time with a similar study in humans that is 3 weeks. The use of a dose of 30 mg/day based on analysis from previous studies. Studies with lycopene dose of 20 mg/day can significantly reduce total cholesterol, but not significantly in high density lipoprotein (HDL) and triglyceride (TG) [8], where studies with a lycopene dose 40 m/day show a positive correlation with total cholesterol, LDL, TG

**144**

and HDL/LDL ratio, but negatively correlated with HDL [9].

The design of this study was a quasi-control experimental design with pre-post test. Subjects were sixty-two Kendal Hospital women employees, aged 35–50 years old, suffering from dyslipidemia but did not suffer from metabolic or degenerative diseases based on the examination of a specialist in internal medicine. Nutrition intake data was measured by 24-hour food recall method for three days in three weeks of the study. Nutritional values were analyzed using the Nutrisurvey program. Food withdrawal is carried out using a food withdrawal form through interviews conducted by nutritionists. Subjects were divided into two groups; group I as the treatment group (30 people) were given with antioxidant supplements (336 g of tomatoes per day) for 21 days and group II (32 people) as the control group. Data on total cholesterol levels, LDL cholesterol levels, HDL cholesterol levels, and triglyceride levels were measured using the chemical analysis system of vitros 300 system by clinical pathology laboratory officers of Regional Hospital Dr. H. Soewondo Kendal, accredited by the government. Measurements were taken before and after supplementation. Blood was taken at 08.00–11.00 Western Indonesian Time (WIT) and immediately checked the total cholesterol level, LDL cholesterol level, HDL cholesterol level, and triglyceride level.

Data was analyzed with Kolmogorov Smirnov test, Pearson test and Mann Whitney test. This research has been legalized by the Health Research Ethics Commission (KEPK) Faculty of Medicine, UNDIP and RSUP DR. Kariadi Semarang, with the issuance of "ETHICAL CLEARANCE" No. 329/EC/FK-RSDK/2014.

#### **3. Results**

In **Table 1**, the results showed that the profile of the study subjects did not show differences in age, BMI, cholesterol intake, Mono Unsaturated Fatty Acid (MUFA) intake, Poly Unsaturated Fatty Acid (PUFA) intake, carbohydrate intake, fiber intake, vitamin A intake, and vitamin C (p > 0.05). But there were differences in the percentage of energy intake and percentage fat intake (p < 0.05).

In **Table 2** significant relationships between energy intake (P = 0.001, r = 0.501), fat intake (P = 0.001, r = 0.574), protein intake (P = 0.008, r = 335) and carbohydrate intake (P = 0.001, r = 0.446) with total cholesterol levels were observed. There was also a significant relationship between the fat intake (P = 0.012, r = 0.318) with LDL levels and there were significant relationships between energy intake (P = 0.005, r = 353), fat intake (P = 0.008, r = 336), protein intake (P = 0.016, r = 305), and carbohydrate intake (P = 0.013, r = 315) with triglyceride levels.

**Table 3** shows the lipid profile of the study subjects before the study. There were no differences between the treatment group and the control group in the total cholesterol levels and the LDL levels (p ≥ 0.05) but, there were differences in the HDL levels and the triglyceride levels (p < 0.05).

#### *Management of Dyslipidemia*


#### **Table 1.**

*Characteristics of research subjects.*


#### **Table 2.**

*Relationship between food intake and research subject profiles.*


#### **Table 3.**

*Lipid profile of subjects before in the treatment group and control group.*

The lipid profile of the subjects after the study can be seen in **Table 4**. There is a difference between the total cholesterol and the LDL levels (p < 0.05) between the treatment group and the control group but no difference in the HDL levels and the triglyceride levels (p ≥ 0.05).

The treatment results can seen in **Table 5**, there were significant differences in total cholesterol (p = 0.001), low lipoprotein (p = 0.001) and triglyceride levels (p = 0.010) in the treatment and the control groups. This shows that there is a significant relationship between the tomato juice supplementation and lipid profile.

**147**

**4. Discussion**

**a***Mann Whitney.*

*Independent Samples t-test. -: decreasing value.*

*b*

**Table 5.**

*aMann Whitney. bIndependent Samples t-test.*

**Table 4.**

*Effect of Tomato Juice Supplements Consumption on the Lipid Profile of Dyslipidemia Patients*

**Variable Control Group (n = 32) Treatment Group (n = 30) Value p**

Chol tot post (gr/dl) 231.3 ± 24.5 202.1 ± 23.7 0.00a HDL post (gr/dl) 54.9 ± 7.5 53.1 ± 9.7 0.23b LDL post (gr/dl) 144.8 ± 23.5 117.5 ± 23.05 0.00b TG post (gr/dl) 156.9 ± 49.73 148.2 ± 56.05 0.55a

**Variable Treatment Group (n = 30) Control Group (n = 32) Value (p)**

d-Chol tot (gr/dl) -29.6 ± 15.2 5.3 ± 12.3 0.001a d-HDL (gr/dl) 4.4 ± 8.9 0.83 ± 4.3 0.070b d-LDL (gr/dl) -32.5 ± 21.8 -0.8 ± 20.1 0.001b d-TG (gr/dl) -41.2 ± 67.3 8 ± 40.9 0.010a

**Average ± SD Average ± SD**

**Rerata Rerata**

The average age of the subjects is 46 years, including middle-aged adults who are at risk of fat tissue with decreased metabolism. The older a person is, the ability of LDL receptors is reduced, causing blood LDL to increase. About 9% of subjects' BMI were obese or an average of 27 kg/m2. Obese adults were more at risk than normal weight adults as they have high total cholesterol, LDL and triglyceride levels which is a major risk factor for coronary heart disease. Obesity, especially central obesity, carries a greater risk for coronary heart disease and other degenerative diseases and dyslipidemia. Excess Very Low Density Lipoprotein (VLDL) is produced in the liver in obese people and subsequently the VLDL is converted to LDL [10]. Due to that, the lipid and lipoprotein profiles of obese people are changed with

Most of the subjects (90.3%) have high energy intake at the average of 2300 kcal. High energy intake results in fat accumulation, especially triglycerides. This will increase VLDL and LDL in blood which will cause an increase in total cholesterol. The fat intake of the study subjects exceeded the nutritional adequacy rate (25–30%) i.e. the average was 34.49% in the treatment group and 35.66% in the control group. When a person consumes more fatty food, fat and triglyceride levels

Significant relationships between energy intake (P = 0.001, r = 0.501), fat intake (P = 0.001, r = 0.574), protein intake (P = 0.008, r = 335) and carbohydrate intake (P = 0.001, r = 0.446) with total cholesterol levels were observed. There was a significant relationship between fat intake (P = 0.012, r = 0.318) with LDL levels. There were significant relationships between energy intake (P = 0.005, r = 353),

in the body will increase. This will increase blood VLDL and LDL [12].

increasing triglycerides and decreasing HDL [11].

*Changes in the lipid profile of the treatment and control group.*

*DOI: http://dx.doi.org/10.5772/intechopen.95239*

*Lipid profile of subjects after in the treatment group and control group.*

*Effect of Tomato Juice Supplements Consumption on the Lipid Profile of Dyslipidemia Patients DOI: http://dx.doi.org/10.5772/intechopen.95239*


#### **Table 4.**

*Management of Dyslipidemia*

imt(kg/m2

*aMann Whitney. bIndependent Samples t-test.*

*Characteristics of research subjects.*

**Table 1.**

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**Table 2.**

*aMann Whitney. bIndependent Samples t-test.*

**Table 3.**

triglyceride levels (p ≥ 0.05).

*Relationship between food intake and research subject profiles.*

*Lipid profile of subjects before in the treatment group and control group.*

The lipid profile of the subjects after the study can be seen in **Table 4**. There is a difference between the total cholesterol and the LDL levels (p < 0.05) between the treatment group and the control group but no difference in the HDL levels and the

**Variable Total Cholesterol Level LDL Level TG Level** Energy intake P = 0.001, r = 0.501 P = 0.110, r = 205 P = 0.005, r = 353 Fat intake P = 0.001,r = 0.574 P = 0.012,r = 0.318 P = 0.008, r = 336 Protein intake P = 0.008, r = 335 P = 0.449,r = 0.098 P = 0.016, r = 305 Carbohydrate intake P = 0.001, r = 0.446 P = 0.325,r = 0.127 P = 0.013 r = 315

**Variable Control Group (n = 32) Treatment Group (n = 30) Value (p) Average ± SD Average ± SD**

Chol tot pre (gr/dl) 225.9 ± 19.3 231.7 ± 24.7 0.45a HDL pre (gr/dl) 53.8 ± 9.6 48.7 ± 11.3 0.04b LDL pre (gr/dl) 144.9 ± 29.2 150.7 ± 23.3 0.22b TG pre (gr/dl) 148.9 ± 69.7 189.4 ± 79.2 0.01a

**Variable Control Group (n = 32) Treatment Group (n = 30) Value (p)**

age (th) 45.2 ± 3.4 46.3 ± 3.9 0.05a

Energy intake (% REE) 124 ± 14.4 116.5 ± 13 0.04b Fat intake (% E) 36 ± 1.7 35 ± 2.7 0.04b Carbohydrate intake (% E) 51 ± 1.6 50.5 ± 3.8 0.30b serat intake (gr) 23.4 ± 2.4 22.8 ± 2.9 0.22b Choles intake (gr) 191.2 ± 27.8 199.3 ± 42.4 0.27b Mufa intake (gr) 24.6 ± 2.7 23.6 ± 2.9 0.14b Pufa intake (gr) 33.4 ± 3.9 31.5 ± 3.6 0.07b vit A intake (μg) 1465.6 ± 295.6 1472.6 ± 260.8 0.49b vit C intake (mg) 123.4 ± 6.8 124.3 ± 9.1 0.75b

**Average ± SD Average ± SD**

) 27 ± 2.1 27.3 ± 3.1 0.96b

The treatment results can seen in **Table 5**, there were significant differences in total cholesterol (p = 0.001), low lipoprotein (p = 0.001) and triglyceride levels (p = 0.010) in the treatment and the control groups. This shows that there is a significant relationship between the tomato juice supplementation and lipid profile. *Lipid profile of subjects after in the treatment group and control group.*


*b*

*Independent Samples t-test. -: decreasing value.*

#### **Table 5.**

*Changes in the lipid profile of the treatment and control group.*

#### **4. Discussion**

The average age of the subjects is 46 years, including middle-aged adults who are at risk of fat tissue with decreased metabolism. The older a person is, the ability of LDL receptors is reduced, causing blood LDL to increase. About 9% of subjects' BMI were obese or an average of 27 kg/m2. Obese adults were more at risk than normal weight adults as they have high total cholesterol, LDL and triglyceride levels which is a major risk factor for coronary heart disease. Obesity, especially central obesity, carries a greater risk for coronary heart disease and other degenerative diseases and dyslipidemia. Excess Very Low Density Lipoprotein (VLDL) is produced in the liver in obese people and subsequently the VLDL is converted to LDL [10]. Due to that, the lipid and lipoprotein profiles of obese people are changed with increasing triglycerides and decreasing HDL [11].

Most of the subjects (90.3%) have high energy intake at the average of 2300 kcal. High energy intake results in fat accumulation, especially triglycerides. This will increase VLDL and LDL in blood which will cause an increase in total cholesterol. The fat intake of the study subjects exceeded the nutritional adequacy rate (25–30%) i.e. the average was 34.49% in the treatment group and 35.66% in the control group. When a person consumes more fatty food, fat and triglyceride levels in the body will increase. This will increase blood VLDL and LDL [12].

Significant relationships between energy intake (P = 0.001, r = 0.501), fat intake (P = 0.001, r = 0.574), protein intake (P = 0.008, r = 335) and carbohydrate intake (P = 0.001, r = 0.446) with total cholesterol levels were observed. There was a significant relationship between fat intake (P = 0.012, r = 0.318) with LDL levels. There were significant relationships between energy intake (P = 0.005, r = 353),

fat intake (P = 0.008, r = 336), protein intake (P = 0.016, r = 305) and carbohydrate intake (P = 0.013, r = 315) with triglyceride levels, which is in line with this research. When compared with a higher-carbohydrate Dietary Approaches to Stop Hypertension (DASH) diet, DASH-type diet with higher protein lowered LDL by 3.3 mg/dL, and triglycerides by 16 mg/dL. Compared with a higher-carbohydrate DASH diet, DASH-type diet with higher unsaturated fat lowered triglycerides by 10 mg/dL [13]. In a meta-analysis of 60 randomized controlled feeding trials, consumption of 1% of calories lowered triglycerides [14].

In a meta-analysis of four prospective cohort studies involving nearly 140,000 subjects, including updated analyses from the two largest studies, a 2 percent increase in energy intake from trans fatty acids was associated with a 23 percent increase in the incidence of CHD (pooled relative risk, 1.23; 95 percent confidence interval, 1.11 to 1.37; P < 0.001) [15]. In meta-analyses of prospective cohort studies, greater consumption of refined complex carbohydrates, starches, and sugars, as assessed by glycemic index or load, was associated with significantly higher risk of CHD and DM. When the highest category (total sedentary time) was compared with the lowest category (short sedentary time), risk of CHD was 36% greater (glycemic load: RR, 1.36; 95% CI, 1.13–1.63) and risk of Diabetes Mellitus was 40% greater (glycemic index: RR, 1.40; 95% CI, 1.23–1.59) [16, 17].

When the highest category was compared with the lowest category, dietary Linoleic Acid (LA) was associated with a 15% lower risk of CHD events (pooled RR, 0.85; 95% confidence intervals, 0.78–0.92; I(2) = 35.5%) and a 21% lower risk of CHD deaths (pooled RR, 0.79; 95% confidence intervals, 0.71–0.89; I(2) = 0.0%). A 5% of energy increment in LA intake replacing energy from saturated fat intake was associated with a 9% lower risk of CHD events (RR, 0.91; 95% confidence intervals, 0.87–0.96) and a 13% lower risk of CHD deaths (RR, 0.87; 95% confidence intervals, 0.82–0.94) [18].

The association suggested that replacing SFA with PUFA rather than MUFA or carbohydrates prevents CHD through various intakes. In comparison, in an analysis of individual levels collected from 11 prospective cohort studies, the specific exchange of PUFA consumption at SFA sites was associated with a lower risk of CHD, with a 13% lower risk for each 5% energy exchange (RR, 0.87; 95% CI, 0.70–0.97 [19].

These findings provided evidence that consuming PUFA as a substitute for SFA reduces the incidence of CHD in RCTs. This also showed that instead of trying to reduce PUFA consumption, a shift towards a larger PUFA population as a substitute for SFA will significantly reduce the level of CHD [20].

The replacement of animal fats, including dairy fat, with vegetable sources of fats and PUFAs may reduce risk of CVD. whereas the 5% energy intake substitution of other animal fat with dairy fat was associated with 6% increased CVD risk (RR: 1.06; 95% CI: 1.02, 1.09) [21].

There were significant differences in total cholesterol (p = 0.001), low lipoprotein (p = 0.001) and triglyceride levels (p = 0.010) in the treatment and control groups. This shows that supplementation of 336 g (350 mL) tomato juice per day for 21 days in women with dyslipidemia with minimal risk can reduce total cholesterol levels by 29.6 g/dL, LDL levels by 32.5 g/dL, triglyceride levels by 41.4 g/dL.

The results of this study are in line with research by Mozaffarian *et al.* [20] which states that these findings are consistent with a meta-analysis of RCTs in which increased PUFA consumption in place of SFAs reduced CHD events, with 10% lower risk for each 5% energy exchange (RR, 0.90; 95% CI, 0.83–0.97.

Among Chinese adults, a higher level of fruit consumption is associated with a lower risk of major cardiovascular disease [22]. Several studies have examined the

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*Effect of Tomato Juice Supplements Consumption on the Lipid Profile of Dyslipidemia Patients*

relationship between antioxidant intake and lipid peroxidation to try to determine which antioxidants play a role in preventing cardiovascular disease. The hydrocarbon carotenoids, including β-carotene and lycopene, are transported primarily in LDL, which puts them in prime position to protect LDL from oxidation [4].

Lycopene may have a cholesterol synthesis-inhibiting effect and may enhance LDL degradation. Available evidence suggests that intimal wall thickness and risk of myocardial infarction are reduced in persons with higher adipose tissue concen-

Treatment with lycopene increased the ejection fraction (EF) from 45.2 ± 3.12 to 51.1 ± 4.63, and it decreased the left ventricular at end-diastole diameter (LVEDd) from 6.52 ± 0.37 mm to 6.18 ± 0.41 mm and the left ventricular at end-systole diameter (LVESd) from 4.29 ± 0.63 to 3.94 ± 0.37 at 28 days post-myocardial infarction. Lycopene attenuated the MI-induced increase in MMP-9 and type I collagen expression, and inhibited p38 activation. Moreover, lycopene decreased the collagen volume fraction in the peri-infarcted zone. The data indicated that lycopene improved the cardiac function and ventricular remodeling by inhibiting p38 activation and MMP-9 expression [24]. Lycopene can suppress VSMCs proliferation, due to inhibiting G1 phase cells entry into the S phase of the cell cycle, related to its antioxidative effect [25], and synthesis of oxidized LDL may be impaired by lycopene [26]. Loana *et al.* [5] reviewed the importance of lycopene in improving vascular function and in the primary and secondary prevention of cardiovascular disorders. The effects shown by lycopene in view of cardiovascular health consist of general antioxidants and anti-inflammatory, antiplatelet, antiapoptotic and antihypertensive properties, ability to improve endothelial function, metabolic profile and ventricular remodeling, reduction in arterial stiffness as well as reduction in atherosclerotic plaque size. Lycopene exerts favorable effects in patients with subclinical atherosclerosis, metabolic syndrome, hypertension, peripheral vascular disease, and several other cardiovascular disorders, but sometimes conflicting results were obtained. Clearly, more and better-designed studies will be necessary to improve our understanding of the positive effects of lycopene on vascular health and to elucidate the involved mechanisms on a molecular level. Future cardiovascular disease prevention strategies might include lycopene-enriched products, lycopene supplementation and new combinations including lycopene. Future studies should be focused on dietary lycopene and its synergistic effects with other dietary components in different study populations, with elevated cardiovascular risk, are highly warranted which might enable development of functional foods useful in prevention and complementary treatment of

There is a significant relationship between energy intake, fat intake, protein intake and carbohydrate intake with total cholesterol levels. So, there is a significant relationship between fat intake with LDL levels and there is a significant relationship between energy intake, fat intake, protein intake and carbohydrate intake with triglyceride levels. About 53.3% of the treatment group subjects had total cholesterol levels normal (<200 mg/dL) after the study and 20% of the treatment group subjects had their LDL levels normal (<100 mg/dL) after the study. Supplementation of 336 g (350 mL) tomato juice per day for 21 days in women with dyslipidemia with minimal risk can reduce total cholesterol levels by 29.6 g/dL,

LDL levels by 32.5 g/dL, and triglyceride levels by 41.4 g/dL.

*DOI: http://dx.doi.org/10.5772/intechopen.95239*

trations of lycopene [23].

cardiovascular disorders [4].

**5. Conclusion**

#### *Effect of Tomato Juice Supplements Consumption on the Lipid Profile of Dyslipidemia Patients DOI: http://dx.doi.org/10.5772/intechopen.95239*

relationship between antioxidant intake and lipid peroxidation to try to determine which antioxidants play a role in preventing cardiovascular disease. The hydrocarbon carotenoids, including β-carotene and lycopene, are transported primarily in LDL, which puts them in prime position to protect LDL from oxidation [4].

Lycopene may have a cholesterol synthesis-inhibiting effect and may enhance LDL degradation. Available evidence suggests that intimal wall thickness and risk of myocardial infarction are reduced in persons with higher adipose tissue concentrations of lycopene [23].

Treatment with lycopene increased the ejection fraction (EF) from 45.2 ± 3.12 to 51.1 ± 4.63, and it decreased the left ventricular at end-diastole diameter (LVEDd) from 6.52 ± 0.37 mm to 6.18 ± 0.41 mm and the left ventricular at end-systole diameter (LVESd) from 4.29 ± 0.63 to 3.94 ± 0.37 at 28 days post-myocardial infarction. Lycopene attenuated the MI-induced increase in MMP-9 and type I collagen expression, and inhibited p38 activation. Moreover, lycopene decreased the collagen volume fraction in the peri-infarcted zone. The data indicated that lycopene improved the cardiac function and ventricular remodeling by inhibiting p38 activation and MMP-9 expression [24]. Lycopene can suppress VSMCs proliferation, due to inhibiting G1 phase cells entry into the S phase of the cell cycle, related to its antioxidative effect [25], and synthesis of oxidized LDL may be impaired by lycopene [26]. Loana *et al.* [5] reviewed the importance of lycopene in improving vascular function and in the primary and secondary prevention of cardiovascular disorders. The effects shown by lycopene in view of cardiovascular health consist of general antioxidants and anti-inflammatory, antiplatelet, antiapoptotic and antihypertensive properties, ability to improve endothelial function, metabolic profile and ventricular remodeling, reduction in arterial stiffness as well as reduction in atherosclerotic plaque size. Lycopene exerts favorable effects in patients with subclinical atherosclerosis, metabolic syndrome, hypertension, peripheral vascular disease, and several other cardiovascular disorders, but sometimes conflicting results were obtained. Clearly, more and better-designed studies will be necessary to improve our understanding of the positive effects of lycopene on vascular health and to elucidate the involved mechanisms on a molecular level. Future cardiovascular disease prevention strategies might include lycopene-enriched products, lycopene supplementation and new combinations including lycopene. Future studies should be focused on dietary lycopene and its synergistic effects with other dietary components in different study populations, with elevated cardiovascular risk, are highly warranted which might enable development of functional foods useful in prevention and complementary treatment of cardiovascular disorders [4].

### **5. Conclusion**

*Management of Dyslipidemia*

dence intervals, 0.82–0.94) [18].

1.06; 95% CI: 1.02, 1.09) [21].

0.70–0.97 [19].

fat intake (P = 0.008, r = 336), protein intake (P = 0.016, r = 305) and carbohydrate intake (P = 0.013, r = 315) with triglyceride levels, which is in line with this research. When compared with a higher-carbohydrate Dietary Approaches to Stop Hypertension (DASH) diet, DASH-type diet with higher protein lowered LDL by 3.3 mg/dL, and triglycerides by 16 mg/dL. Compared with a higher-carbohydrate DASH diet, DASH-type diet with higher unsaturated fat lowered triglycerides by 10 mg/dL [13]. In a meta-analysis of 60 randomized controlled feeding trials,

In a meta-analysis of four prospective cohort studies involving nearly 140,000

When the highest category was compared with the lowest category, dietary Linoleic Acid (LA) was associated with a 15% lower risk of CHD events (pooled RR, 0.85; 95% confidence intervals, 0.78–0.92; I(2) = 35.5%) and a 21% lower risk of CHD deaths (pooled RR, 0.79; 95% confidence intervals, 0.71–0.89; I(2) = 0.0%). A 5% of energy increment in LA intake replacing energy from saturated fat intake was associated with a 9% lower risk of CHD events (RR, 0.91; 95% confidence intervals, 0.87–0.96) and a 13% lower risk of CHD deaths (RR, 0.87; 95% confi-

The association suggested that replacing SFA with PUFA rather than MUFA or carbohydrates prevents CHD through various intakes. In comparison, in an analysis of individual levels collected from 11 prospective cohort studies, the specific exchange of PUFA consumption at SFA sites was associated with a lower risk of CHD, with a 13% lower risk for each 5% energy exchange (RR, 0.87; 95% CI,

These findings provided evidence that consuming PUFA as a substitute for SFA reduces the incidence of CHD in RCTs. This also showed that instead of trying to reduce PUFA consumption, a shift towards a larger PUFA population as a substitute

The replacement of animal fats, including dairy fat, with vegetable sources of fats and PUFAs may reduce risk of CVD. whereas the 5% energy intake substitution of other animal fat with dairy fat was associated with 6% increased CVD risk (RR:

There were significant differences in total cholesterol (p = 0.001), low lipoprotein (p = 0.001) and triglyceride levels (p = 0.010) in the treatment and control groups. This shows that supplementation of 336 g (350 mL) tomato juice per day for 21 days in women with dyslipidemia with minimal risk can reduce total cholesterol

Among Chinese adults, a higher level of fruit consumption is associated with a lower risk of major cardiovascular disease [22]. Several studies have examined the

levels by 29.6 g/dL, LDL levels by 32.5 g/dL, triglyceride levels by 41.4 g/dL. The results of this study are in line with research by Mozaffarian *et al.* [20] which states that these findings are consistent with a meta-analysis of RCTs in which increased PUFA consumption in place of SFAs reduced CHD events, with 10% lower risk for each 5% energy exchange (RR, 0.90; 95% CI, 0.83–0.97.

subjects, including updated analyses from the two largest studies, a 2 percent increase in energy intake from trans fatty acids was associated with a 23 percent increase in the incidence of CHD (pooled relative risk, 1.23; 95 percent confidence interval, 1.11 to 1.37; P < 0.001) [15]. In meta-analyses of prospective cohort studies, greater consumption of refined complex carbohydrates, starches, and sugars, as assessed by glycemic index or load, was associated with significantly higher risk of CHD and DM. When the highest category (total sedentary time) was compared with the lowest category (short sedentary time), risk of CHD was 36% greater (glycemic load: RR, 1.36; 95% CI, 1.13–1.63) and risk of Diabetes Mellitus was

40% greater (glycemic index: RR, 1.40; 95% CI, 1.23–1.59) [16, 17].

for SFA will significantly reduce the level of CHD [20].

consumption of 1% of calories lowered triglycerides [14].

**148**

There is a significant relationship between energy intake, fat intake, protein intake and carbohydrate intake with total cholesterol levels. So, there is a significant relationship between fat intake with LDL levels and there is a significant relationship between energy intake, fat intake, protein intake and carbohydrate intake with triglyceride levels. About 53.3% of the treatment group subjects had total cholesterol levels normal (<200 mg/dL) after the study and 20% of the treatment group subjects had their LDL levels normal (<100 mg/dL) after the study. Supplementation of 336 g (350 mL) tomato juice per day for 21 days in women with dyslipidemia with minimal risk can reduce total cholesterol levels by 29.6 g/dL, LDL levels by 32.5 g/dL, and triglyceride levels by 41.4 g/dL.

*Management of Dyslipidemia*
