**2.2.6 Statistical analysis**

The initial wheezing episodes were plotted by the Kaplan–Meier method, and p-values were calculated with the log-rank test. Pearson's chisquared test was used to analyze the incidence of asthma. The Th1/Th2 ratio, total IgE level, and eosinophil count were compared between before and after treatment using the paired t-test. It was employed to determine the significance of differences between the two treatments. Results were expressed as the mean ± s.e., and p-values of <0.05 were considered to indicate statistical significance.
