**Abbreviations**


**37**

**Author details**

Siti Nurul Hidayah†

, Manasi Gaikwad†

† These authors have equally contributed as first authors.

\*Address all correspondence to: hschluet@uke.de

provided the original work is properly cited.

Mass Spectrometric Proteomics, Institute of Clinical Chemistry and Laboratory Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

, Laura Heikaus and Hartmut Schlüter\*

*Preparing Proteoforms of Therapeutic Proteins for Top-Down Mass Spectrometry*

PPS protein-purification parameter screening system

LC–MS/MS liquid chromatography coupled to tandem mass spectrometry

*DOI: http://dx.doi.org/10.5772/intechopen.89644*

MS mass spectrometry mAbs monoclonal antibodies

IRMPD infrared multiple photon dissociation IEX ion exchange chromatography LC liquid chromatography

rhIL recombinant human interleukin rTP recombinant therapeutic protein RPLC reversed phase liquid chromatography SEC size exclusion chromatography TDMS top-down mass spectrometry UVPD ultraviolet photodissociation

*Preparing Proteoforms of Therapeutic Proteins for Top-Down Mass Spectrometry DOI: http://dx.doi.org/10.5772/intechopen.89644*

