**7. Overview of an efficient anther culture protocol used in eggplant**

The stages of an eggplant anther culture protocol (applied in TUBITAK TEYDEB Project No. 68989 conducted by Antalya Tarim Co. R&D Center) which can be used effectively and practically in breeding studies were briefly summarized in **Figures 1**–**9**.

#### **Figure 1.** *Donor plant growing under biotic and abiotic stress-free conditions.*

**81**

**Figure 4.**

*Pretreatments (temperature applications) and incubation of cultures.*

**Figure 3.**

*medium preparation.*

*Development of Androgenesis Studies on Eggplant (*Solanum melongena *L.) in Turkey…*

*Culture of anthers under aseptic conditions after appropriate surface sterilization treatment and nutrient* 

*DOI: http://dx.doi.org/10.5772/intechopen.88299*

**Figure 2.** *Suitable bud morphology for eggplant anther culture.*

*Development of Androgenesis Studies on Eggplant (*Solanum melongena *L.) in Turkey… DOI: http://dx.doi.org/10.5772/intechopen.88299*

#### **Figure 3.**

*Sustainable Crop Production*

eggplant so far.

through organogenesis [47, 81]. However, an efficient direct embryogenesis protocol have not yet been developed in the isolated microspore culture studies in

**7. Overview of an efficient anther culture protocol used in eggplant**

The stages of an eggplant anther culture protocol (applied in TUBITAK TEYDEB Project No. 68989 conducted by Antalya Tarim Co. R&D Center) which can be used effectively and practically in breeding studies were briefly summarized in **Figures 1**–**9**.

**80**

**Figure 2.**

**Figure 1.**

*Suitable bud morphology for eggplant anther culture.*

*Donor plant growing under biotic and abiotic stress-free conditions.*

*Culture of anthers under aseptic conditions after appropriate surface sterilization treatment and nutrient medium preparation.*

**Figure 4.** *Pretreatments (temperature applications) and incubation of cultures.*

#### **Figure 5.** *Direct androgenesis and haploid embryo formation.*

**83**

**Figure 8.**

*greenhouse.*

**Figure 7.**

*Development of Androgenesis Studies on Eggplant (*Solanum melongena *L.) in Turkey…*

*Plants obtained from anther culture at the different ploidy levels. Application of chromosome doubling in the* 

*DOI: http://dx.doi.org/10.5772/intechopen.88299*

*Acclimatization of in vitro regenerated plantlets.*

**Figure 6.** *In vitro regeneration and development of haploid plantlets.*

*Development of Androgenesis Studies on Eggplant (*Solanum melongena *L.) in Turkey… DOI: http://dx.doi.org/10.5772/intechopen.88299*

**Figure 7.** *Acclimatization of in vitro regenerated plantlets.*

*Sustainable Crop Production*

**82**

**Figure 6.**

**Figure 5.**

*Direct androgenesis and haploid embryo formation.*

*In vitro regeneration and development of haploid plantlets.*

**Figure 8.** *Plants obtained from anther culture at the different ploidy levels. Application of chromosome doubling in the greenhouse.*

**Figure 9.** *Colchicine treatment with lanolin. Fruits on the DH branches.*
