**5.10 Advantages of in-situ bioremediation**


## **5.11 Limitation of in-situ bioremediation**

Depending on specific site, some contaminants may not be absolutely transformed to harmless products.

If transformation stops at an intermediate compound, the intermediate may be more toxic and/or mobile than parent compound some are recalcitrant contaminants cannot be biodegradable.

When incorrectly applied, injection wells may become blocked by profuse microbial growth due to addition of nutrients, electron donor and electron acceptor.

Heavy metals and organic compounds concentration inhibit activity of indigenous microorganisms.

In-situ bioremediation usually required microorganism's acclimatization, which may not develop for spills and recalcitrant compounds.
