**Abstract**

*Staphylococcus aureus* is a pathogenic microorganism that causes gastrointestinal diseases due to the production of enterotoxins. The study of lactic acid bacteria such as *Lactobacillus plantarum* has generated interest due to its ability to generate secondary metabolites against pathogenic microorganisms. In this work, the stability of the antimicrobial activity of the *L. plantarum* supernatants was evaluated by means of a well diffusion test. The supernatants were stored at 25 ± 1.0°C for a period of 20 weeks. A significant difference (P < 0.05) was observed in the antimicrobial activity of *L. plantarum* supernatants between time 0 and after 20 weeks of storage, although the ability to inhibit *Staphylococcus aureus* was still observed during the storage time.

**Keywords:** lactic acid bacteria, storage, antimicrobial activity, stability
