**Author details**

*Design and Manufacturing*

**Conflict of interest**

PAMI—ROTEIRO/0328/2013 (NO. 022158), and MATIS (CENTRO-01-0145- FEDER-000014-3362). It is also funded by the projects insitu.Biomas (POCI-01-0247-FEDER-017771), Bone2Move (PTDC/CVT-CVT/31146/2017), and

Stimuli2BioScaffolds (PTDC/EME-SIS/32554/2017).

The authors declare no conflict of interest.

BMMSCs bone marrow mesenchymal stem cells

hBMSCs human bone marrow-derived mesenchymal stem cells

**Appendices and nomenclature**

GF growth factors Hap hydroxyapatite

AM additive manufacturing β-TCP beta-tricalcium phosphate

BTE bone tissue engineering. CAD computer-aided design EBM electron beam melting ECM extracellular matrix

FDA Food and Drug Administration FFF fused filament fabrication

hMSCs human mesenchymal stem cells hESCs human embryonic stem cells MSC mesenchymal stem cells PCL poly(ε-caprolactone) PLA polylactic acid

rBMMSCs rat bone marrow stromal cell

SLA stereolithography SLM selective laser melting SLS selective laser sintering TCP tricalcium phosphate TE tissue engineering

**174**

Cristiana Fernandes1 , Carla Moura1 , Rita M.T. Ascenso2 , Sandra Amado1 , Nuno Alves1 and Paula Pascoal-Faria1 \*

1 Centre for Rapid and Sustainable Product Development of the Polytechnic Institute of Leiria, Leiria, Portugal

2 Computer Science and Communication Research Centre, Polytechnic Institute of Leiria, Leiria, Portugal

\*Address all correspondence to: paula.faria@ipleiria.pt

© 2020 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
