**3.3 Bioreactor start-up and operating conditions**

Several trails performed with the AMBB, under batch conditions were carried out during 15 days. The reactor was inoculated with 15 dm3 of fresh winery wastewater, from the vintage period and with 0.15 dm3 of acclimated biomass, obtained during the treatment of winery wastewater, in the previously year. Samples from the mixed liquor were daily taken for physico-chemical characterisation. The aerated flow was 2 dm3 min-1. The operating temperature was 20-30ºC. The recirculation of the mixed liquor started with 20 min hour-1, with a flow of 40 dm3 min-1 and then was changed to 5 min hour-1.
