**5. Factors affecting biosynthesis of nanoparticles**

Manufacturing nanoparticles is a very critical process where the environmental factor is temperature and pressure; light intensity plays an important role in the synthesis of nanoparticles. Parameters such as sample leaf extract concentration, AgNO3 concentration, reaction temperature, reaction time, pH reaction and different light reactions play a key role in the creation of silver nanoparticles of varying size, shape and dimension. Similarly, the light conditions such as sun light, blue light, red light, bulb light, tube light and in dark devoid of light effects are examined for nanoparticle biosynthesis. Several parameters that play a crucial role in the synthesis of the particular nanoparticles required have been addressed briefly. Such parameters are modified in order to produce the desired size of nanoparticles for the study of specific activity. Temperature is one of the most important parameters for nanoparticle synthesis.
