**5.3 Effect of pH**

A major influence of the reaction (pH) is its ability to change the electrical loads of biomolecules, which may affect their ability to cap and stabilize and, consequently, the growth of nanoparticles [21]. In general, in the course of research work experimentation, our observations suggest that, if the pH of the reactant is acidic, the transition from light green to dark brown will take a much longer time, indicating that the acidic medium is not appropriate for nanoparticle synthesis. The size of nanoparticles is expected to be higher in the acidic medium than in the basic media, which is also reported in different studies. It was our observation that particle sizes at pH 3 were greater than those at pH 8 with regular spherical shape, both of which were observed in the TEM analysis. The alkaline pH condition facilitated the reduction and stabilizing capacity of antioxidants in the leaf extract. During optimization, the pH spectrum could be tested from 3 to 11 pH and potential spectroscopic UV tests could detect changes in absorption.
