*4.3.4 Influence of sound*

In this process, the protocol is the same, and it is the reaction process carried under different conditions using selective instruments. The sound waves consist of longitudinal waves and transverse waves in solids that can be easily produced using a sound source such as the vibrating diaphragm of a stereo speaker. Therefore, the noise or acoustic environment is used to study different aspects of nanoparticles. Sound waves are used to isolate biological nanoparticles from bio-liquid samples. However, sound effects on the synthesis of bio-nanoparticles have not been studied. So, we are trying to provide some insight on the impact of sound on bio-nanoparticle synthesis. We are very eager to know the effect of sound including (jazz/rock/gaze/ pop song) and the level of sound frequency on the synthesis of silver nanoparticles. Once the nanoparticles are synthesized, they are used for characterization by means of the available instruments, which will define nanoparticles size, shape, surface load, aggregates, colloidal, capping agent detection, reducing agent confirmation and conjugation (its ability to combine) with another polymer [18]. **Table 2** indicates the possible conditions under which the manufacture of silver nanoparticles is feasible.
