**7. Conclusions**

Unique properties of plasmonic nanostructures open exciting new possibilities for increasing the sensitivity of molecular chirality. There are several common approaches among different experimental and theoretical studies benefiting in biosensing. Despite moderate CD enhancement factors, the formation of complexes with small achiral nanoparticles can be useful for detecting chiral organic molecules and peptides.

An alternative approach based on the formation/destruction of chiral plasmon dimers, constructed using target-specific aptamers, makes it possible to detect a wide range of biomolecules, including enzymes and proteins, in complex biological environments and even inside living cells. For detection of supramolecular biological structures, such as amyloid fibrils, the formation of self-organizing three-dimensional chiral assemblies of achiral plasmon nanoparticles can be used. This fact is very useful for early diagnosis of various concomitant diseases. Sophisticated fabrication and a moderate increase in optical chirality make it difficult to use three-dimensional chiral plasmon nanostructures, while flat chiral metasurfaces that can be conveniently obtained using lithographic methods demonstrate a high potential for ex-vivo detection of chiral biomolecules, further allowing the study of their tertiary and domain structure.

Currently, the field of chiral plasmonic biosensing is of great interest for various biosensor applications [103]. One can expect new developments in this area and unique results in the future. Due to the theoretical basis developed by scientists in understanding the nature of the interaction of chiral molecules with plasmoninduced near-fields, the creation of more advanced nanostructures requires even greater care. Variation of the geometry and assembly of nanostructures, as well as the use of new, primarily dielectric [104] and hybrid materials and alloys with specified dielectric functions [105]—these are the ways of increasing sensitivity of chiral biosensors. Moreover, the use of combinations of various types of chiral nanostructures allows the simultaneous detection of chirality at various scales or the detection of various chiral molecules in the complex biological media.
