**9. Conclusion**

Silver nanoparticle fabrication is a single pot reaction that transforms water soluble components such as AgNO3, plant-based capping and stabilizing agent (secondary metabolites/phytochemicals) into water-insoluble components, that is, nanoparticles. Biological methods are the best methods due to their advantages such as low cost preparation, avoidance of environmental contamination, nontoxic by-products and adequate supply of samples, which can be scaled up for large production. Various conditions, such as dark, sunlight, microwave oven, autoclave, sonication, different wavelengths of light, heating, boiling, etc., and various other sources, methods, may apply as required by experiments. In addition, conditions such as dark, sun, heating, boiling, sonication and autoclave can be checked for the size and shape of colloidal nanoparticles. The stability of nanoparticles depends on different parameters such as leaf extract concentration, AgNO3, reaction temperature, pH, light and stirring time to optimize and determine the size and shape of nanoparticles. Potential nanoparticles have been applied in many cross-disciplinary fields, from agriculture to medicine, but their effect, fate and accumulation on plants remain a mystery.
