**7. In vitro assay**

The potential prospectus on the capacity of nanoparticles is tested via in vitro assay that based on Kirby-Bauer technique. The inherent potential of nanoparticles is used on various applications in laboratory as well as in industries. There are various ways to trace the inhibition property of silver nanoparticles. The minimum inhibition concentration of dried powder nanoparticles is important to determine the dose of nanoparticles against pathogens, and the zone of inhibition by silver nanoparticles is compared with the standard antibiotics. The concentration at which silver nanoparticles shows some effect to the microbe or fungi is determined by several methods such as agar well diffusion, disc diffusion and toxicity assays that are generally used for detecting the minimum concentration of silver nanoparticles for its antimicrobial/antifungal activity, larvicidal activity, anti-inflammatory, antiplatelet activity, anti-angiogenesis, antiviral, antilarva and pupicidal activity [25].
