*The Components of Functional Nanosystems and Nanostructures DOI: http://dx.doi.org/10.5772/intechopen.92027*

by this method have more ability to convert poorly soluble and poorly absorbed drugs into better deliverables.

Particularly particle size and surface distribution properties are essential characteristic analyzes for the characterization of nanospheres. Because particle sizes and surface distributions also illuminate the properties of in vivo distribution, biological fate, toxicity and targeting ability. For the characterization of these properties, scanning electron microscopy (SEM), transmission electron microscopy (TEM), photon correlation spectroscopy methods are used. The Zeta potential analysis provides insightful information about load stability and particle collection. Zetasizer is used for this analysis. Fourier transform infrared spectroscopy (FTIR) analyses are performed to reveal the chemical bonding between the active substance and the polymers. The physical state of the active substance in the nanospheres is determined by differential scanning calorimetry (DSC) analysis after the lyophilization of the nanospheres. It can be applied many analysis methods such as in vitro drug release studies, drug release kinetics and stability study. Stability studies help to examine the effects of nanospheres on the physicochemical parameters of their formulations. In this way, suitable storage conditions are determined. Absorption and storage at room temperatures are performed for approximately 6 months and the observed changes in physicochemical parameters are recorded. Appropriate storage conditions are determined according to the results obtained. **Table 1** shows the intended use of the nanospheres synthesized according to the literature. **Figure 4** schematizes the synthesized gold nanospheres.

Nanospheres are used effectively in controlled drug delivery systems, tumortargeted treatment methods, tumor-targeted treatment methods, epithelial cell therapy, genetic engineering studies, treatment methods targeting blood–brain barriers, cosmetics and many other areas [1].
