**5. Conclusions**

The determination of selenium is of great importance from the point of view of understanding its metabolism and its potential benefits for human health. Due to the variety of selenium species and low concentrations in which it occurs in environmental samples, liquid chromatography coupled to ICP-MS is the most powerful method for speciation analysis of selenium. However, other techniques, for example, HILIC-MS/MS, have also been successfully applied in the speciation analysis of selenium. It should be highlighted that for such analysis all the analytical steps like selection of separation and detection method and the optimization of separation parameters (eluent type and composition, mobile phase additives) should be optimized to avoid the coelution of selenium species.

The recent application of liquid chromatography for selenium separation in plants (vegetables samples) is shown in **Table 3**. For sure new chromatographic strategies will be described in the literature in the nearest future.


#### **Table 3.**

*Examples of the recent HPLC application for selenium speciation in plant materials.*
