**6. Crystallization**

On a Perkin-Elmer spectrometer, infrared spectra were recorded at room temperature in an interval of 500–4000 cm<sup>1</sup> .

The compound was crystallized by dissolving the 1–10 phenanthroline hydrate (1 g) in mixture solution of ethanol-water (V/V = 1:1). The solution was preserved at room temperature under agitation during 4 h. After a slow evaporation in the interior of 2 days, transparent prism crystals were obtained.

*Strongly Fluorescent Heterocyclic Molecule: Crystallography, 3D Hydrogen-Bonded, Fluorescence… DOI: http://dx.doi.org/10.5772/intechopen.90271*

The crystals formed were washed and filtered using 20 ml of water. The most important infrared wavelength (cm<sup>1</sup> ):

(1): 3400 (vs), 3084 (m), 1632 (w), 1617 (w), 1575 (s), 1564 (m), 1502 (s), 1443 (w), 1414 (vs), 1340 (w), 1285 (vw), 1208 (w), 1132 (m), 1082 (w), 1031 (w), 987 (m), 860 (vw), 764 (w), 733 (vs).

## **7. Programmes**

Computer programmes by Bruker *APEX2* (2006) [22]; *SAINT* (Bruker, 2006) [22]; *SAINT*, *SIR2002* [23], and *SHELXL2008* [24]; *WinGX* [25]; *Mercury* Version 1.4 [26]
