**2.6 Fragment analysis**

For fragment analysis, a mixture of 1 μl of the PCR product with 8.5 μl deionised formamide and 0.5 μl of the size standard ILS 600 (Promega, Madison, WI, USA) was prepared and was subjected to capillary electrophoresis in an ABI Prism 3100-Avant Genetic Analyser (Applied Biosystems, Foster City, CA, USA). The injection times varied between 4 and 36 s. Fragment length and fluorescence intensity were analysed using GeneMapper Software (Applied Biosystems, Foster City, CA, USA), and the detection threshold was set for 50 RFU. The selection of informative STR loci was based on previously described experiences [29, 30], where in such loci donor and recipient alleles should be individually distinguishable [15, 18].
