**5. Conclusions**

The identification of β-tubulin bundles that surround the DNA nuclei in telophase suggests a mechanism of entrapment similar to a "hand-closure movement" that allows, along with the inter-MTOC spindle, the distribution of genetic material between the two newly formed nuclei.

The mitosis of *Entamoeba histolytica* trophozoites is a type of intranuclear pleuromitosis, closed or open? modified, since three spindles are formed: two radial and one inter MTOCs. In addition, these are found within the nucleus. It remains to be discovered if the spindle that apparently is outside the nucleus has a nuclear envelope or not.

**227**

**Author details**

Eduardo Gómez-Conde1

Luis David Gómez-Cortes4

Workers, Mexico City, Mexico

provided the original work is properly cited.

Autonomous University of Puebla, Mexico

of the National Polytechnic Institute, Mexico City, Mexico

Merciful Autonomous University of Puebla, Mexico

\*Address all correspondence to: gom\_cond@yahoo.com

\*, Miguel Ángel Vargas Mejía<sup>2</sup>

1 Laboratory of Research at Immunobiology, School of Medicine, Merciful

and Tayde Guerrero-González<sup>5</sup>

2 Molecular Biomedicine Department, Center for Research and Advanced Studies

3 Laboratory of Research at Experimental Immunology, School of Medicine,

5 Hospital "1ro of October", Institute of Security and Social Services for State

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

4 School of Biology, Merciful Autonomous University of Puebla, Mexico

, María Alicia Díaz y Orea3

,

*The Mitosis of* Entamoeba histolytica *Trophozoites DOI: http://dx.doi.org/10.5772/intechopen.88734*

*The Mitosis of* Entamoeba histolytica *Trophozoites DOI: http://dx.doi.org/10.5772/intechopen.88734*

*Parasitology and Microbiology Research*

KCL-hypotonization.

between the two newly formed nuclei.

**5. Conclusions**

envelope or not.

After karyokinesis, cytokinesis begins; the trophozoite with two nuclei begins to divide by narrowing the cytoplasm in its middle part [5]. This process is relatively slow and occurs gradually through stretching with intervals of rest. The cytoplasm of the middle part of the trophozoite thins until it forms a very thin filament which then breaks and results in two trophozoites of *Entamoeba histolytica* with a nucleus in each of them. It is still unknown whether cytoplasmic DNA [38] duplicates during mitosis of trophozoites or if it is only genetic material with other functions. The description and observations realized in the different phases of mitosis in *E. histolytica* trophozoites using the phase-contrast microscopy technique are consistent with those described by acridine orange vital staining and transmission electron microscopy [3–6]. Peripheral RNA chromatin and central DNA chromatin behaved in a similar manner to the acridine orange staining described [5]. The data described and observed with the anti-β-tubulin antibodies of *E. histolytica* regarding the radial microtubule bundles correlate with the observations described with transmission electron microscopy of nuclei in prophase and prometaphase [3, 4, 6]. However, there is a discrepancy about the number of spindles that are present and observed between the two nuclei in formation [2, 3, 6). The description of an inter-MTOC spindle independent of radial spindles was based on its observation with immunofluorescence (**Figure 8a**). An observation that brings forth new ways of studying the nuclear division in *E. histolytica* trophozoites is the presence of a green fluorescent internuclear cord obtained with the acridine orange staining and

The identification of β-tubulin bundles that surround the DNA nuclei in telophase suggests a mechanism of entrapment similar to a "hand-closure movement" that allows, along with the inter-MTOC spindle, the distribution of genetic material

The mitosis of *Entamoeba histolytica* trophozoites is a type of intranuclear pleuromitosis, closed or open? modified, since three spindles are formed: two radial and one inter MTOCs. In addition, these are found within the nucleus. It remains to be discovered if the spindle that apparently is outside the nucleus has a nuclear

**226**
