**7. Safety issues of nanoparticles**

Nano-biotechnology is the fine amalgamation of multi disciplines of science. In pharmaceutical field, the scientists and researchers are trying to overcome the demerits of existing drug molecules with the aid of this wonderful technology. There are many expected potential benefits in bank. In spite of that safety is the main concern with the in- vivo application of nano engineered medicines yet.

It has been revealed that while production or use of nano medicines, such particles can easily get inside the human body and ultimately blood stream and then in various vital organs; where they exert unintended adverse effects, sometimes these effects are fetal for the user.

An in- vivo study on monkeys and rats have disclosed the aggregation of carbon and manganese nano materials in the olfactory bulb, which has produced severe pathological consequences pertaining to nervous and respiratory system.

Several animal studies have shown such sort of abnormal pathological disturbances.

In order to combat with this problem firstly, an efficient probe will have to develop for thorough assessment of risk associated with the use of nano medicine. Second and the most significant challenge would be to develop authentic validation protocol for testing nano medicines in animals so as to get the data of toxic effects in early stage of testing. A restructured, compact and integrated regulatory approach is much needed to look into the expected risks for delivering the promising and safe medicines [2].
