**7. Generalization of the research**

Taking into consideration all research described in the chapter, it is necessary to generalize that isolation of mesophilic SRB from environmental samples (water, soil, swamp, etc.) and intestinal samples can be similar, although swamps and feces are required to determine dry matter of the samples. It is important to purify a positive sample of SRB from other satellite microorganisms such as *Clostridium*, *Bacteroides*, *Pseudomonas*, etc. With this aim, obtained SRB mixed cultures should be 3–5 times repassed to the modified Postgate agar medium with sulfite which inhibit the growth other microorganisms. Ability to growth of SRB mixed culture with high sulfite concentration allows to eliminate (purify SRB) from other microorganisms which can be in close interactions with SRB. The cultivation conditions depend on sample from where it is isolated. The key criteria for identification based on physiological and biochemical characteristics are the morphology of bacterial
