**6.1 Purification of fructose**

The separation of FOS and fructose is frequently accomplished by reckonable chromatographic techniques. In dietetic products, optimal FOS separation is done by implementing glass-packed precoated silica gel with sodium acetate. Liquid chromatography (LC) with acetonitrile as a mobile phase is executed to purify nonstructural carbohydrates such as sugars and FOS with 3–19 degrees of polymerization. Auxiliary cost-effective methods exploiting activated charcoal fixed bed column with 80% degree of purification and 97.8% recovery of Fructose are superfluously proficient [43]. Purified fructose is assessed by diverse techniques such as NMR, MALDI-MS, MALDITOF, GC-MS, and ESI-MS [46]. The prebiotic fructose metabolism in microorganisms can be premeditated through microarrays [43].

### **6.2 Commercial applications of fructose**

Pure fructose along with FOSs is finely specified to exist in voluminous natural foods. Gigantic companies are manufacturing these extensively applicable healthy and calorie-free products via hydrolyzing inulin weeds by exploiting microbial inulinases. Few lucrative applications of fructose emphasized in the review [47].
