**Figure 8.**

*The general scheme of isolation, purification, and identification of mesophilic sulfate-reducing bacteria.*

**37**

*Isolation and Purification of Sulfate-Reducing Bacteria DOI: http://dx.doi.org/10.5772/intechopen.86786*

sulfate-reduction, such as *DsrAB* and А*prBA*.

**8. Conclusions**

**Acknowledgements**

(MUNI/A/0902/2018).

**Conflict of interest**

**Author details**

Ivan Kushkevych

The authors declare no conflict of interest.

Masaryk University in Brno, Czech Republic

provided the original work is properly cited.

Department of Experimental Biology, Faculty of Science,

\*Address all correspondence to: kushkevych@mail.muni.cz

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

cells, ability to form of spores, sulfate reduction to hydrogen sulfide, lactate oxidation to acetate or CO2, use of other organic compounds as an electron donor and carbon sources, etc. The general scheme of isolation, purification, and identifica-

For identifications of SRB based on morphological, physiological, and biochemical characteristics, two Bergey's Manuals [10, 11] are recommended. Moreover, all isolated SRB species should be confirmed by the sequence analysis of the 16S rRNA gene by using universal primers or primers of functional genes of dissimilatory

The methods of sample selections from water, soil, swamp, and feces of humans or animals and from biopsy material and the process of SRB isolation and purifications are similar, although cultivation conditions may differ. Identification based on physiological and biochemical properties is a complex process, and many other factors must be considered. For this identification, Bergey's Manuals are recommended to be used. The sequence analysis of the 16S rRNA gene should confirm the

It is of vital importance to obtain new strains of the SRB from various ecotopes and identify them and study their growth and physiological and biochemical properties. Aside from that, the process of dissimilatory sulfate reduction by SRB and the production of hydrogen sulfide should be investigated in order to clarify the etiological role of these bacteria in the nature and in the development of various diseases.

identification process based on physiological and biochemical properties.

This study was supported by Grant Agency of the Masaryk University

tion of mesophilic sulfate-reducing bacteria is presented in **Figure 8**.

*Isolation and Purification of Sulfate-Reducing Bacteria DOI: http://dx.doi.org/10.5772/intechopen.86786*

cells, ability to form of spores, sulfate reduction to hydrogen sulfide, lactate oxidation to acetate or CO2, use of other organic compounds as an electron donor and carbon sources, etc. The general scheme of isolation, purification, and identification of mesophilic sulfate-reducing bacteria is presented in **Figure 8**.

For identifications of SRB based on morphological, physiological, and biochemical characteristics, two Bergey's Manuals [10, 11] are recommended. Moreover, all isolated SRB species should be confirmed by the sequence analysis of the 16S rRNA gene by using universal primers or primers of functional genes of dissimilatory sulfate-reduction, such as *DsrAB* and А*prBA*.
