**Abstract**

*Staphylococcus epidermidis*, member of the group of coagulase-negative staphylococci, belongs to an opportunistic pathogen. It is reported that the major pathogenicity of *S. epidermidis* is attributed to its biofilm formed on the surface of infected tissues, which enhances bacterial resistance to antibiotics. Thus, how to inhibit biofilm formation and screening biofilm inhibitors will have great value in reducing bacterial drug-resistance, which is beneficial to prevent and treat biofilmassociated infections. In this chapter, we present the current knowledge on formation, antibiotic resistance, and control strategies of *S. epidermidis* biofilm. First, biofilm formation in *S. epidermidis*, including factors involved in different phases in the process of biofilm, is analyzed. Second, the mechanisms of antibiotic resistance in *S. epidermidis* biofilms, such as poor antibiotic penetration, slow growth, and formation of persister cells, are introduced. Finally, control strategies to *S. epidermidis* biofilm formation are provided.

**Keywords:** *Staphylococcus epidermidis*, biofilm, antibiotic resistance, biofilm inhibition
