**Abstract**

*Staphylococcus aureus* is one of the most common pathogens that cause recurrent, chronic, and biofilm-related diseases. Biofilms are the major form of bacterial structures capable of secreting polysaccharides that provide intrinsic protection against environmental stress like high concentrations of antibiotics. This, along with the emergence of multidrug-resistant strains, has made *S. aureus* infections a worldwide problem as a result of the inefficiency of the conventional medications. Plant essential oils (EOs) are an important source for drug discovery and pharmaceutical development due to their diverse biological activities, such as antimicrobial agents. The EOs' microbicide action is extensively reported at the scientific literature and frequently associated with bioactive molecules, such as aldehydes and terpenes. However, the ability of some EOs to inhibit biofilm formation has been poorly explored and it is still unclear how they could be applied in specific treatments against well-known infections. Therefore, this chapter will address virulence factors and biofilm formation of *S. aureus*, as well as bioprospecting of essential oil as a promising source in the search for new bioactive compounds employed in the fight against this microorganism.

**Keywords:** antibiofilm activity, biofilm-related diseases, essential oil, natural products, *Staphylococcus aureus*
