*3.3.3 Time before harvest*

RJ should be harvested 48 h after larva transfer to artificial queen cell cups. This is because the RJ harvested 48 h after larva transfer is very active although the amount is smaller than the amount harvested after 72 h. As described below (Section 3.2.1), the content of 10-HDA in RJ is significantly higher in the harvest after 48 h than that after 72 h. The various effects on humans and experimental animals are also known to be more excellent in the former harvest. In the case of 72 h harvest, the operation efficiency is higher since a larger amount of RJ is pooled in each queen cell cup and the operation can be performed once every 3 days, but such the RJ harvested is far less active compared to that after 48 h harvest (**Figures 7** and **8**).
