Health Benefits of Dietary Protein throughout the Life Cycle

*Jamie I. Baum, Elisabet Børsheim, Brittany R. Allman and Samuel Walker*

## **Abstract**

Dietary protein intake and the associated health benefits continue to be a subject of great debate. The quantity of protein consumed, the quality or source of protein consumed, and the timing of protein intake throughout the day all play a role in determining the health benefits of dietary protein. Research suggests that intake of dietary protein above the dietary recommendations has health benefits throughout the lifecycle. This book chapter describes the dietary recommendations for protein intake throughout pregnancy, childhood, and adulthood and the associated health benefits with protein intake above the dietary guidelines at each stage of life.

**Keywords:** dietary protein, dietary guidelines, children, adults, health benefits

## **1. Introduction**

Proteins are chains of amino acids which are involved in nearly every process in the body. Proteins function as enzymes, transcription factors, binding proteins, transmembrane transporters and channels, hormones, receptors, structural proteins, and signaling proteins [1]. However, the primary role of protein in the diet is to provide amino acids required for the synthesis of new proteins. We especially rely on dietary protein to provide the nine essential amino acids, which cannot be synthesized in the body. Protein intake greater than the dietary recommendations may prevent sarcopenia [2], help maintain energy balance [3], improve bone health [4–7] and cardiovascular function [8–10], and aid in wound healing [11]. This chapter focuses on the role of dietary protein, and the associated health benefits, throughout the life cycle.

## **2. Dietary recommendations for protein intake**

The current dietary recommendations for protein intake include the estimated average requirement (EAR) [12] and the recommended dietary allowance [12]. For daily protein intake, the EAR for dietary protein is 0.66 g kg<sup>−</sup><sup>1</sup> day<sup>−</sup><sup>1</sup> , and the RDA is 0.8 g kg<sup>−</sup><sup>1</sup> day<sup>−</sup><sup>1</sup> for all adults over 18 years of age. This can become confusing when trying to make recommendations for individuals at different stages of life. Even the Food and Nutrition Board recognizes a difference between what is recommended in the RDA and the level of protein intake needed for optimal health [12]. Therefore, there is a third recommendation for protein called the acceptable daily

macronutrient range (ADMR) [13, 14]. The ADMR includes a recommendation for protein intakes ranging from 10 to 35% of daily energy (e.g., calorie intake), which makes the ADMR easier to use when developing dietary recommendations for protein [12].

## **3. Dietary protein intake in adults**

A majority of the adult population in the United States exceeds the minimum recommendations for protein intake [15]. The current dietary protein intake in the United States is approximately 82 g d<sup>−</sup><sup>1</sup> for men and 67 g d<sup>−</sup><sup>1</sup> for women [16]. **Table 1** details the current protein intake as percent of energy intake in the United States based on sex and age. A majority of dietary protein comes from animal protein (46%), followed by plant protein (30%), dairy (16%), and mixed foods (8%) [16]. There is increasing evidence indicating that consuming dietary protein at levels above the current RDA (0.8 g dietary protein kg body weight<sup>−</sup><sup>1</sup> day<sup>−</sup><sup>1</sup> ) may be beneficial for children, adults, older adults, and physically active individuals [17]. For example, protein intake above the RDA may help reduce the risk of chronic diseases such as obesity, cardiovascular disease, type 2 diabetes, osteoporosis, and sarcopenia [13, 17]. However, high protein intake without a subsequent decrease in carbohydrates attenuates the beneficial effects of dietary protein [18].


**Table 1.**

*Percentage macronutrient intake in the United States by sex and age [19].*

## **4. Dietary protein intake in children**

Adequate dietary protein intake is essential to support cellular integrity, growth, and physical function. Although protein malnutrition is not prevalent in the United States, there is little research on optimal protein requirements for health benefits in children. Current EARs are based on the factorial method and the nitrogen balance technique. The factorial method incorporates the estimated nitrogen (protein) requirement plus the rate of protein deposition and an estimate of the efficiency of protein utilization [20] which is derived from adult dietary protein needs [12]. By using the indicator amino acid oxidation method in a group of healthy children 6–11 years old, it was found that the mean and population-safe (upper 95% CI) protein requirements were 1.3 and 1.55 g kg<sup>−</sup><sup>1</sup> day<sup>−</sup><sup>1</sup> , respectively. This is higher than the 2005 DRI for protein (0.76 and 0.95 g kg<sup>−</sup><sup>1</sup> day<sup>−</sup><sup>1</sup> , respectively) [12]. A similar study using the nitrogen balance technique also found that protein requirements in children in this age range are above current recommendations at 1.2 g kg<sup>−</sup><sup>1</sup> day<sup>−</sup><sup>1</sup> [21]. These higher estimated protein requirements in children seem to be in line with current protein consumption patterns in different pediatric age groups. For instance, children 2–3 years old are currently daily consuming ~3.6 g/kg of ideal body weight, children 4–8 years old are currently

**119**

kg<sup>−</sup><sup>1</sup>

day<sup>−</sup><sup>1</sup>

(EAR) and 1.1 g kg<sup>−</sup><sup>1</sup>

protein needs to be 1.2 g kg<sup>−</sup><sup>1</sup>

day<sup>−</sup><sup>1</sup>

at 30–38 weeks [31]. Both nonpregnant women of childbearing age (20–44 years)

day<sup>−</sup><sup>1</sup>

(RDA) [12]. However, newer studies found

day<sup>−</sup><sup>1</sup>

at 11–20 weeks, increasing to 1.52 g kg<sup>−</sup><sup>1</sup>

*Health Benefits of Dietary Protein throughout the Life Cycle*

**5. Dietary protein intake in pregnant women**

ideal body weight<sup>−</sup><sup>1</sup>

for children is still under debate [22]. There are racial/ethnic differences in protein consumption in children (2–18 years old). For example, non-Hispanic black children eat about 5% below, non-Hispanic white children eat about 3% below, Hispanic children eat about 2% below, and Asian children eat less than 1% below

Although the currently established recommendations for protein intake in children may be lower than the requirements, the effect of diets higher in protein (e.g., 30% of total energy intake) in children is unclear [22]. Several studies have alluded to the potential benefit of higher protein intake dietary practices. For instance, diets higher in protein with a low glycemic index can be protective against obesity in children aged 5–18 years [23], and diets higher in protein can lead to smaller waist circumference, blood pressure, insulin, and serum cholesterol than lower-protein diets in children from the same age group. A recent cohort analysis found that protein intake in 8-year-olds is associated with higher fat-free mass [24], and an additional cohort analysis found that at ages 11, 15, and 22 years, protein intake is inversely associated with early adulthood BMI. However, protein intake at 2 years was positively associated with BMI and lean mass at age 22 [25], suggesting there are conflicting results regarding the benefits of increased dietary protein in

Pregnancy is a period of rapid tissue growth during a short period of time. Maternal tissues, including breast, uterine, and adipose tissues, blood volume, and extracellular fluids, account for the largest amount of protein accretion during pregnancy at 60%. The remaining 40% of protein accretion occurs within the amniotic fluid, fetus, and placenta [26, 27]. In fact, protein needs to increase soon after conception to support tissue growth and development, maintenance of maternal homeostasis, and lactation preparation [27–29]. These alterations occur in an exponential way and only in response to adequate total energy intake. This means that protein deposition does not significantly change in the first trimester compared to pre-pregnancy, but increases during the second trimester and significantly increases to the highest levels of protein deposition in the third trimester. This variable period of growth makes it difficult to define recommendations regarding protein requirements. Thus, although current recommendations suggest constant protein intake throughout the duration of pregnancy, pregnancy may actually require an increase in protein intake throughout gestation to support adequate growth, although further research is needed. There are several benefits of protein intake during pregnancy including adequate maternal weight gain within recommendations, lower early pregnancy BMI, and decreased postpartum weight [30]. Although the benefits of increased protein intake during pregnancy are apparent as stated above, protein requirements during pregnancy are difficult to measure. This is due to the involved nature of some of the techniques used to measure protein requirements. Therefore, the current dietary protein recommendations during pregnancy are based on factorial estimates of recommendations for healthy, nonpregnant populations. Pregnancy protein needs have been derived from the EAR and RDA for healthy, nonpregnant populations and are set to 0.88 g

ideal body weight<sup>−</sup><sup>1</sup>

, and children 9–13 years old are con-

[15]; however, the optimal protein intake

*DOI: http://dx.doi.org/10.5772/intechopen.91404*

consuming ~2.6 g kg<sup>−</sup><sup>1</sup>

the EAR for protein [15].

suming ~1.6 g kg<sup>−</sup><sup>1</sup>

children.

*The Health Benefits of Foods - Current Knowledge and Further Development*

protein [12].

**Protein**

**Table 1.**

**3. Dietary protein intake in adults**

the United States is approximately 82 g d<sup>−</sup><sup>1</sup>

**4. Dietary protein intake in children**

*Percentage macronutrient intake in the United States by sex and age [19].*

day<sup>−</sup><sup>1</sup>

macronutrient range (ADMR) [13, 14]. The ADMR includes a recommendation for protein intakes ranging from 10 to 35% of daily energy (e.g., calorie intake), which makes the ADMR easier to use when developing dietary recommendations for

A majority of the adult population in the United States exceeds the minimum recommendations for protein intake [15]. The current dietary protein intake in

**Table 1** details the current protein intake as percent of energy intake in the United States based on sex and age. A majority of dietary protein comes from animal protein (46%), followed by plant protein (30%), dairy (16%), and mixed foods (8%) [16]. There is increasing evidence indicating that consuming dietary protein

be beneficial for children, adults, older adults, and physically active individuals [17]. For example, protein intake above the RDA may help reduce the risk of chronic diseases such as obesity, cardiovascular disease, type 2 diabetes, osteoporosis, and sarcopenia [13, 17]. However, high protein intake without a subsequent decrease in

**Age Total Men Women**

20–44 years 15.7 16.1 15.3 45–64 years 15.8 16.0 15.7 65–74 years 16.3 16.6 16.1 75 years and older 15.7 16.1 15.3

Adequate dietary protein intake is essential to support cellular integrity, growth, and physical function. Although protein malnutrition is not prevalent in the United States, there is little research on optimal protein requirements for health benefits in children. Current EARs are based on the factorial method and the nitrogen balance technique. The factorial method incorporates the estimated nitrogen (protein) requirement plus the rate of protein deposition and an estimate of the efficiency of protein utilization [20] which is derived from adult dietary protein needs [12]. By using the indicator amino acid oxidation method in a group of healthy children 6–11 years old, it was found that the mean and population-safe

tively) [12]. A similar study using the nitrogen balance technique also found that protein requirements in children in this age range are above current recommen-

children seem to be in line with current protein consumption patterns in different pediatric age groups. For instance, children 2–3 years old are currently daily consuming ~3.6 g/kg of ideal body weight, children 4–8 years old are currently

[21]. These higher estimated protein requirements in

(upper 95% CI) protein requirements were 1.3 and 1.55 g kg<sup>−</sup><sup>1</sup>

This is higher than the 2005 DRI for protein (0.76 and 0.95 g kg<sup>−</sup><sup>1</sup>

at levels above the current RDA (0.8 g dietary protein kg body weight<sup>−</sup><sup>1</sup>

carbohydrates attenuates the beneficial effects of dietary protein [18].

for men and 67 g d<sup>−</sup><sup>1</sup>

for women [16].

day<sup>−</sup><sup>1</sup>

day<sup>−</sup><sup>1</sup>

day<sup>−</sup><sup>1</sup>

, respectively.

, respec-

) may

**118**

dations at 1.2 g kg<sup>−</sup><sup>1</sup>

consuming ~2.6 g kg<sup>−</sup><sup>1</sup> ideal body weight<sup>−</sup><sup>1</sup> , and children 9–13 years old are consuming ~1.6 g kg<sup>−</sup><sup>1</sup> ideal body weight<sup>−</sup><sup>1</sup> [15]; however, the optimal protein intake for children is still under debate [22]. There are racial/ethnic differences in protein consumption in children (2–18 years old). For example, non-Hispanic black children eat about 5% below, non-Hispanic white children eat about 3% below, Hispanic children eat about 2% below, and Asian children eat less than 1% below the EAR for protein [15].

Although the currently established recommendations for protein intake in children may be lower than the requirements, the effect of diets higher in protein (e.g., 30% of total energy intake) in children is unclear [22]. Several studies have alluded to the potential benefit of higher protein intake dietary practices. For instance, diets higher in protein with a low glycemic index can be protective against obesity in children aged 5–18 years [23], and diets higher in protein can lead to smaller waist circumference, blood pressure, insulin, and serum cholesterol than lower-protein diets in children from the same age group. A recent cohort analysis found that protein intake in 8-year-olds is associated with higher fat-free mass [24], and an additional cohort analysis found that at ages 11, 15, and 22 years, protein intake is inversely associated with early adulthood BMI. However, protein intake at 2 years was positively associated with BMI and lean mass at age 22 [25], suggesting there are conflicting results regarding the benefits of increased dietary protein in children.

## **5. Dietary protein intake in pregnant women**

Pregnancy is a period of rapid tissue growth during a short period of time. Maternal tissues, including breast, uterine, and adipose tissues, blood volume, and extracellular fluids, account for the largest amount of protein accretion during pregnancy at 60%. The remaining 40% of protein accretion occurs within the amniotic fluid, fetus, and placenta [26, 27]. In fact, protein needs to increase soon after conception to support tissue growth and development, maintenance of maternal homeostasis, and lactation preparation [27–29]. These alterations occur in an exponential way and only in response to adequate total energy intake. This means that protein deposition does not significantly change in the first trimester compared to pre-pregnancy, but increases during the second trimester and significantly increases to the highest levels of protein deposition in the third trimester. This variable period of growth makes it difficult to define recommendations regarding protein requirements. Thus, although current recommendations suggest constant protein intake throughout the duration of pregnancy, pregnancy may actually require an increase in protein intake throughout gestation to support adequate growth, although further research is needed. There are several benefits of protein intake during pregnancy including adequate maternal weight gain within recommendations, lower early pregnancy BMI, and decreased postpartum weight [30].

Although the benefits of increased protein intake during pregnancy are apparent as stated above, protein requirements during pregnancy are difficult to measure. This is due to the involved nature of some of the techniques used to measure protein requirements. Therefore, the current dietary protein recommendations during pregnancy are based on factorial estimates of recommendations for healthy, nonpregnant populations. Pregnancy protein needs have been derived from the EAR and RDA for healthy, nonpregnant populations and are set to 0.88 g kg<sup>−</sup><sup>1</sup> day<sup>−</sup><sup>1</sup> (EAR) and 1.1 g kg<sup>−</sup><sup>1</sup> day<sup>−</sup><sup>1</sup> (RDA) [12]. However, newer studies found protein needs to be 1.2 g kg<sup>−</sup><sup>1</sup> day<sup>−</sup><sup>1</sup> at 11–20 weeks, increasing to 1.52 g kg<sup>−</sup><sup>1</sup> day<sup>−</sup><sup>1</sup> at 30–38 weeks [31]. Both nonpregnant women of childbearing age (20–44 years)

and pregnant women consume at or above the current recommendations of protein intake [32, 33]. One study [31] found that pregnant women consume the same amount of protein in early pregnancy (1.44 ± 0.30 g kg<sup>−</sup><sup>1</sup> day<sup>−</sup><sup>1</sup> ) as they do in late pregnancy (1.47 ± 0.53 g kg<sup>−</sup><sup>1</sup> day<sup>−</sup><sup>1</sup> ), not taking fluid retention and changes in body composition into account. These findings support others that have noted little overall change in dietary protein patterns from early to late pregnancy [33]. Collectively, these findings demonstrate that pregnant women meet the recommendations for dietary protein intake. Improvements may potentially be made to increase dietary protein requirements as pregnancy progresses.

## **6. Protein quality versus protein quantity**

An important factor to consider when incorporating protein into the diet is how the source of dietary protein (e.g., protein derived from animal or plant sources) affects nutrient intake, nutrient adequacy, and diet quality [13, 34, 35]. Proteins with differing amino acid profiles exhibit varied digestion and absorption rates [36–38], and amino acid profiles depend directly on the quality and quantity of the dietary protein [37]. For example, the digestion and absorption rates of fast- (e.g., whey) versus slow (e.g., casein)-digesting proteins need to be taken into consideration when developing protein recommendations. One study provided young, healthy subjects with either a whey protein meal (30 g) or a casein meal (43 g) (both contained the same amount of leucine [one of the BCAAs]) and measured whole-body protein synthesis. Researchers determined that the subjects consuming the whey (fast) protein meal had a high, rapid increase in plasma amino acids, while subjects consuming the casein (slow) protein meal had a prolonged plateau of EAA [39]. In addition, the chemical structure and the presence of anti-nutritional compounds such as phytic acid within the protein source can influence digestion and amino acid availability [40]. Compared to animal sources, plant proteins are shown to have a lower anabolic impact on muscle; however, the reduced ability to elicit anabolic effects can be overcome by increasing protein intake and increasing the content of leucine [41].

Whether or not the amino acid source is derived from the whole protein or a mixture of free amino acids can also influence the rate of muscle protein synthesis [42]. For example, when older subjects were given either an EAA mixture (15 g) or a whey protein supplement (13.6 g) after an overnight fast, subjects consuming the EAA mixture had higher mixed muscle fractional synthetic rate [42], which is often associated with increases in muscle mass. The differing response could be due to the differing leucine content between the supplements (EAA, 2.8 g leucine, and whey, 1.8 g leucine) or because the EAA supplement was composed of individual amino acids while the whey protein supplement was intact protein. These subtle differences could influence the rate of appearance of the amino acids into blood circulation and thus the protein synthetic response.

Another potential confounder of the protein synthetic response of various proteins is the form or texture of the protein itself, such as ground beef versus a beef steak [43]. When, older men consumed 135 g of protein as either ground beef or as a beef steak, the amino acids from the ground beef appeared more rapidly in the circulation than the amino acids from the beef steak. Whole-body protein balance was higher after consumption of the ground beef versus the beef steak. However, 6 h after the beef meals, muscle protein synthesis was not different [43]. Nonetheless, these data support that the form of the protein that is being consumed impacts digestion, absorption, and the rate of appearance of amino acids into circulation [35].

**121**

*Health Benefits of Dietary Protein throughout the Life Cycle*

The timing of dietary protein intake has received ample attention in the past several decades. Adults typically consume the majority of their protein intake at dinner (38 g) versus breakfast (13 g) [44]. However, recent research suggests that ingestion of more than 30 g of protein in a test meal does not further stimulate the effect of dietary protein on muscle protein synthesis [45]. This had led to discussion related to optimal timing of protein intake. For example, distributing protein intake throughout the day, timing of protein around nighttime eating, and protein eating at breakfast are all areas of increased interest. In general, research covering these topics is performed in young, healthy populations, or aging populations, and very

few, if any, studies have been conducted in children and pregnant women.

Breakfast is often recognized as the most important meal of the day [46–48]. However, there is debate as to what defines the ideal breakfast meal [47], in addition to a lack of strong evidence to define which nutrients should be represented at breakfast [47]. A recent commentary published by the American Academy of Nutrition and Dietetics suggests that protein-containing foods (e.g., eggs, lean meat, and low-fat dairy products) should be included in breakfast meals [47]. Literature supports diets higher in protein aid in the treatment of chronic, metabolic diseases such as obesity, type 2 diabetes, and heart disease and have been shown to increase EE, improve satiety, regulate glycemic control, and improve body

Eating protein at night and immediately before bedtime has received substantial attention in the past decade. Although past common knowledge would claim that eating before bed precipitates negative effects on health and body composition, more recent studies show that there may be many metabolic, health, and body composition-related benefits [50]. Much of the previous research claiming the negative effects of nighttime eating was performed in shift workers [51], populations with night eating syndrome, who consume ≥50% of daily calories after dinner [52], and epidemiological data [53]. Although some of the negative effects of nighttime eating in these populations may include high BMI and abdominal obesity [54]; increased triglyceride concentration, dyslipidemia, and impaired glucose tolerance [55]; impaired kidney function [56]; and increased carbohydrate oxidation and decreased fat oxidation [57], many other factors need to be taken into consideration. For example, these populations are awake during abnormal hours and report sleep disturbances [58, 59]. In fact, the duration of sleep is inversely related to BMI [60, 61]. These populations also consume significantly more carbohydrate, protein, and fat throughout the day. Nonetheless, it is clear that eating large amounts of energy in the evening hours, in particular when the energy is carbohydrate- and fat-laden, may not be beneficial for health and body composition outcomes. However, much more evidence has shown that eating a small protein snack (~200 kcal) before bed may elicit significant benefits. Improved muscle protein recovery, muscle mass, and strength gains mediated by enhanced overnight and next-morning muscle protein synthesis have been shown to be enhanced with 40 g of casein protein supplementation in elderly [62] and recreationally active men [63]. These effects are particularly enhanced when this dietary practice is added to the practice of resistance exercise [63]. In addition, reported hunger is lower and

*DOI: http://dx.doi.org/10.5772/intechopen.91404*

**7. Timing of protein intake**

**7.1 Protein intake at breakfast**

composition (reviewed in [13, 14, 34, 49]).

**7.2 Protein intake in the evening**

## **7. Timing of protein intake**

*The Health Benefits of Foods - Current Knowledge and Further Development*

day<sup>−</sup><sup>1</sup>

amount of protein in early pregnancy (1.44 ± 0.30 g kg<sup>−</sup><sup>1</sup>

protein requirements as pregnancy progresses.

**6. Protein quality versus protein quantity**

pregnancy (1.47 ± 0.53 g kg<sup>−</sup><sup>1</sup>

the content of leucine [41].

tion and thus the protein synthetic response.

and pregnant women consume at or above the current recommendations of protein intake [32, 33]. One study [31] found that pregnant women consume the same

composition into account. These findings support others that have noted little overall change in dietary protein patterns from early to late pregnancy [33]. Collectively, these findings demonstrate that pregnant women meet the recommendations for dietary protein intake. Improvements may potentially be made to increase dietary

An important factor to consider when incorporating protein into the diet is how the source of dietary protein (e.g., protein derived from animal or plant sources) affects nutrient intake, nutrient adequacy, and diet quality [13, 34, 35]. Proteins with differing amino acid profiles exhibit varied digestion and absorption rates [36–38], and amino acid profiles depend directly on the quality and quantity of the dietary protein [37]. For example, the digestion and absorption rates of fast- (e.g., whey) versus slow (e.g., casein)-digesting proteins need to be taken into consideration when developing protein recommendations. One study provided young, healthy subjects with either a whey protein meal (30 g) or a casein meal (43 g) (both contained the same amount of leucine [one of the BCAAs]) and measured whole-body protein synthesis. Researchers determined that the subjects consuming the whey (fast) protein meal had a high, rapid increase in plasma amino acids, while subjects consuming the casein (slow) protein meal had a prolonged plateau of EAA [39]. In addition, the chemical structure and the presence of anti-nutritional compounds such as phytic acid within the protein source can influence digestion and amino acid availability [40]. Compared to animal sources, plant proteins are shown to have a lower anabolic impact on muscle; however, the reduced ability to elicit anabolic effects can be overcome by increasing protein intake and increasing

Whether or not the amino acid source is derived from the whole protein or a mixture of free amino acids can also influence the rate of muscle protein synthesis [42]. For example, when older subjects were given either an EAA mixture (15 g) or a whey protein supplement (13.6 g) after an overnight fast, subjects consuming the EAA mixture had higher mixed muscle fractional synthetic rate [42], which is often associated with increases in muscle mass. The differing response could be due to the differing leucine content between the supplements (EAA, 2.8 g leucine, and whey, 1.8 g leucine) or because the EAA supplement was composed of individual amino acids while the whey protein supplement was intact protein. These subtle differences could influence the rate of appearance of the amino acids into blood circula-

Another potential confounder of the protein synthetic response of various proteins is the form or texture of the protein itself, such as ground beef versus a beef steak [43]. When, older men consumed 135 g of protein as either ground beef or as a beef steak, the amino acids from the ground beef appeared more rapidly in the circulation than the amino acids from the beef steak. Whole-body protein balance was higher after consumption of the ground beef versus the beef steak. However, 6 h after the beef meals, muscle protein synthesis was not different [43]. Nonetheless, these data support that the form of the protein that is being consumed impacts digestion, absorption, and the rate of appearance of amino acids into

day<sup>−</sup><sup>1</sup>

), not taking fluid retention and changes in body

) as they do in late

**120**

circulation [35].

The timing of dietary protein intake has received ample attention in the past several decades. Adults typically consume the majority of their protein intake at dinner (38 g) versus breakfast (13 g) [44]. However, recent research suggests that ingestion of more than 30 g of protein in a test meal does not further stimulate the effect of dietary protein on muscle protein synthesis [45]. This had led to discussion related to optimal timing of protein intake. For example, distributing protein intake throughout the day, timing of protein around nighttime eating, and protein eating at breakfast are all areas of increased interest. In general, research covering these topics is performed in young, healthy populations, or aging populations, and very few, if any, studies have been conducted in children and pregnant women.

## **7.1 Protein intake at breakfast**

Breakfast is often recognized as the most important meal of the day [46–48]. However, there is debate as to what defines the ideal breakfast meal [47], in addition to a lack of strong evidence to define which nutrients should be represented at breakfast [47]. A recent commentary published by the American Academy of Nutrition and Dietetics suggests that protein-containing foods (e.g., eggs, lean meat, and low-fat dairy products) should be included in breakfast meals [47]. Literature supports diets higher in protein aid in the treatment of chronic, metabolic diseases such as obesity, type 2 diabetes, and heart disease and have been shown to increase EE, improve satiety, regulate glycemic control, and improve body composition (reviewed in [13, 14, 34, 49]).

#### **7.2 Protein intake in the evening**

Eating protein at night and immediately before bedtime has received substantial attention in the past decade. Although past common knowledge would claim that eating before bed precipitates negative effects on health and body composition, more recent studies show that there may be many metabolic, health, and body composition-related benefits [50]. Much of the previous research claiming the negative effects of nighttime eating was performed in shift workers [51], populations with night eating syndrome, who consume ≥50% of daily calories after dinner [52], and epidemiological data [53]. Although some of the negative effects of nighttime eating in these populations may include high BMI and abdominal obesity [54]; increased triglyceride concentration, dyslipidemia, and impaired glucose tolerance [55]; impaired kidney function [56]; and increased carbohydrate oxidation and decreased fat oxidation [57], many other factors need to be taken into consideration. For example, these populations are awake during abnormal hours and report sleep disturbances [58, 59]. In fact, the duration of sleep is inversely related to BMI [60, 61]. These populations also consume significantly more carbohydrate, protein, and fat throughout the day. Nonetheless, it is clear that eating large amounts of energy in the evening hours, in particular when the energy is carbohydrate- and fat-laden, may not be beneficial for health and body composition outcomes.

However, much more evidence has shown that eating a small protein snack (~200 kcal) before bed may elicit significant benefits. Improved muscle protein recovery, muscle mass, and strength gains mediated by enhanced overnight and next-morning muscle protein synthesis have been shown to be enhanced with 40 g of casein protein supplementation in elderly [62] and recreationally active men [63]. These effects are particularly enhanced when this dietary practice is added to the practice of resistance exercise [63]. In addition, reported hunger is lower and

satiety is higher, and resting energy expenditure is higher the following morning after a small protein snack compared to a noncaloric placebo [50, 62]. Chronically (4 weeks) there are also reports of decreased blood pressure, decreased arterial stiffness [64], and a greater decrease in body fat in overweight and obese women when consuming nighttime protein [65, 66]. Importantly, these benefits are accompanied by no significant alterations in overnight or next-morning lipolysis, fat oxidation, substrate utilization, or any blood markers in obese men or resistancetrained young women [67].

#### **7.3 Distribution of protein intake throughout the day**

Current research demonstrates that even distribution of protein intake throughout the day is more effective at stimulating a 24-h protein synthesis compared to an uneven distribution [68, 69]. This is supported by data from a longitudinal study on nutrition and aging, which found that even distribution of daily protein intake across meals is independently associated with greater muscle strength and higher muscle mass in older adult, but is not associated with loss in muscle mass [70] or mobility [71] over 2–3 years. However, there are some studies that fail to confirm the importance of spreading protein intake out over the course of the day [71, 72]. Additional studies have compared pulse feeding (72% of daily protein at lunch) versus protein being evenly distributed over four daily meals in hospitalized older patients for 6 weeks [73, 74]. These studies found that pulse feeding of protein increased postprandial amino acid bioavailability [75] and increased lean mass [74] compared to spreading protein intake throughout the day. Taken together, the optimal timing and distribution of protein intake still need to be determined.

#### **8. Dietary protein and health**

#### **8.1 Dietary protein and obesity**

Obesity is a major public health concern [76] and is associated with the development of metabolic diseases such as cardiovascular disease, nonalcoholic fatty liver disease, and type 2 diabetes mellitus in both children and adults [77, 78]. Obesity is defined as having a body mass index (BMI) (weight in kilograms divided by height in centimeters squared) greater than or equal to 30.0. In 2015–2016, the prevalence of obesity (**Table 2**) in the United States was 39.6 for adults and 18.4% for youth [76]. Obesity also impacts racial and ethnic groups differently. For example, non-Hispanic black and Hispanic adults and youth have higher rates of obesity compared to non-Hispanic white and Asian populations [79].

A primary factor in controlling and preventing obesity and associated chronic diseases is through diet, for example, diets higher in protein [13, 14, 80, 81]. Diets higher in protein (>30% of energy intake) have been shown to improve body composition [82], improve glycemic response [81, 83–85], increase satiety [85–87], and increase postprandial energy metabolism [88, 89], which are all mediating factors of weight loss.

#### **8.2 Dietary protein and sarcopenia**

Sarcopenia is the term for age-associated loss of muscle mass and function [35]. The loss of muscle function associated with sarcopenia is often referred to as dynapenia [90]. A loss or reduction in skeletal muscle function often leads to increased morbidity and mortality either directly, or indirectly, via the development of

**123**

RDA [16, 44].

*\$*

*\**

**Table 2.**

*Health Benefits of Dietary Protein throughout the Life Cycle*

**(percent)**

Youth, 2–19 18.5 19.1 17.8 Young children, 2–5 13.9 14.3 13.5 Youth, 6–11 18.4\$ 20.4\$ 16.3 Adolescents, 12–19 20.6\$ 20.2 20.9\$ Adults, 20+ 39.6 37.9 41.1 Young adults, 20–39 35.7 34.8 36.5 Middle-aged adults, 40–59 42.8\* 40.8\* 44.7\* Older adults, 60+ 41.0 38.5 43.1

**Boys or men (percent)**

hormones and/or nutrients, and a sedentary lifestyle.

*Prevalence of obesity in the United States by age group and sex [76].*

older patients to recover from disease and trauma [91].

elderly adults [91, 95, 97]. The RDA of 0.8 g kg<sup>−</sup><sup>1</sup>

aging recommend a protein intake between 1.2 and 2.0 g kg<sup>−</sup><sup>1</sup>

secondary diseases such as diabetes, obesity, and cardiovascular disease [91]. The causes of sarcopenia include poor nutrition, diminished responsiveness to anabolic

The loss in muscle mass observed with aging is often accompanied by an increase in fat mass [92], which can happen even in the absence of changes in BMI [35]. The loss in muscle mass results in a decrease in basal metabolic rate (BMR) or the amount of caloric energy we use while at rest [93]. The loss of muscle mass induces a 2–3% decrease in BMR per decade after the age of 20 and a 4% decline in BMR per decade after the age of 50 [93, 94]. Muscle loss and subsequent reduction

in metabolic rate contribute to obesity that accompanies the aging process. Several studies identify protein as a key nutrient for aging adults [2, 95]. Low protein intake is linked to a decrease in physical ability in aging adults [96]. However, protein intake greater than the dietary guidelines may prevent sarcopenia [96], help maintain BMR [3], improve bone health [4–7], and improve cardiovascular function [8–10]. These benefits of increasing protein in the diet may improve function and quality of life in healthy older adults, as well as improve the ability of

Currently, the dietary recommendations for protein intake are the same for all healthy adults above the age of 19. However, experts in the field of protein and

mendations and reflects a value at the lowest end of the AMDR. It is estimated that 38% of adult men and 41% of adult women have dietary protein intakes below the

protein intake to older adults [34, 35]. There are three important aspects to take into consideration when recommending a protein source: (1) the characteristics of the specific protein, such as the amount of essential amino acids (EAA); (2) the food matrix in which the protein is consumed, for example, as part of a beverage or a complete meal; and (3) the characteristics of the individuals consuming the food, including health status, physiological status, and energy balance [34]. In addition, the difference in digestibility and bioavailability of a protein can impact the quantity of protein that needs to be ingested to meet metabolic needs; this is especially important in older adults since gastric motility and nutrient absorption decrease with age. The speed of protein digestion and absorption of amino acids

Both protein amount and source are important to consider when recommending

day<sup>−</sup><sup>1</sup>

day<sup>−</sup><sup>1</sup>

is well below these recom-

or higher for

**Girls or women (percent)**

*DOI: http://dx.doi.org/10.5772/intechopen.91404*

**Age group (years) Total** 

*Significantly different from young children.*

*Significantly different from young adults.*

#### *Health Benefits of Dietary Protein throughout the Life Cycle DOI: http://dx.doi.org/10.5772/intechopen.91404*


*Significantly different from young children.*

*\* Significantly different from young adults.*

#### **Table 2.**

*The Health Benefits of Foods - Current Knowledge and Further Development*

**7.3 Distribution of protein intake throughout the day**

trained young women [67].

**8. Dietary protein and health**

**8.1 Dietary protein and obesity**

to non-Hispanic white and Asian populations [79].

**8.2 Dietary protein and sarcopenia**

satiety is higher, and resting energy expenditure is higher the following morning after a small protein snack compared to a noncaloric placebo [50, 62]. Chronically (4 weeks) there are also reports of decreased blood pressure, decreased arterial stiffness [64], and a greater decrease in body fat in overweight and obese women when consuming nighttime protein [65, 66]. Importantly, these benefits are accompanied by no significant alterations in overnight or next-morning lipolysis, fat oxidation, substrate utilization, or any blood markers in obese men or resistance-

Current research demonstrates that even distribution of protein intake throughout the day is more effective at stimulating a 24-h protein synthesis compared to an uneven distribution [68, 69]. This is supported by data from a longitudinal study on nutrition and aging, which found that even distribution of daily protein intake across meals is independently associated with greater muscle strength and higher muscle mass in older adult, but is not associated with loss in muscle mass [70] or mobility [71] over 2–3 years. However, there are some studies that fail to confirm the importance of spreading protein intake out over the course of the day [71, 72]. Additional studies have compared pulse feeding (72% of daily protein at lunch) versus protein being evenly distributed over four daily meals in hospitalized older patients for 6 weeks [73, 74]. These studies found that pulse feeding of protein increased postprandial amino acid bioavailability [75] and increased lean mass [74] compared to spreading protein intake throughout the day. Taken together, the optimal timing and distribution of protein intake still need to be determined.

Obesity is a major public health concern [76] and is associated with the development of metabolic diseases such as cardiovascular disease, nonalcoholic fatty liver disease, and type 2 diabetes mellitus in both children and adults [77, 78]. Obesity is defined as having a body mass index (BMI) (weight in kilograms divided by height in centimeters squared) greater than or equal to 30.0. In 2015–2016, the prevalence of obesity (**Table 2**) in the United States was 39.6 for adults and 18.4% for youth [76]. Obesity also impacts racial and ethnic groups differently. For example, non-Hispanic black and Hispanic adults and youth have higher rates of obesity compared

A primary factor in controlling and preventing obesity and associated chronic diseases is through diet, for example, diets higher in protein [13, 14, 80, 81]. Diets higher in protein (>30% of energy intake) have been shown to improve body composition [82], improve glycemic response [81, 83–85], increase satiety [85–87], and increase postprandial energy metabolism [88, 89], which are all mediating factors

Sarcopenia is the term for age-associated loss of muscle mass and function [35]. The loss of muscle function associated with sarcopenia is often referred to as dynapenia [90]. A loss or reduction in skeletal muscle function often leads to increased morbidity and mortality either directly, or indirectly, via the development of

**122**

of weight loss.

*Prevalence of obesity in the United States by age group and sex [76].*

secondary diseases such as diabetes, obesity, and cardiovascular disease [91]. The causes of sarcopenia include poor nutrition, diminished responsiveness to anabolic hormones and/or nutrients, and a sedentary lifestyle.

The loss in muscle mass observed with aging is often accompanied by an increase in fat mass [92], which can happen even in the absence of changes in BMI [35]. The loss in muscle mass results in a decrease in basal metabolic rate (BMR) or the amount of caloric energy we use while at rest [93]. The loss of muscle mass induces a 2–3% decrease in BMR per decade after the age of 20 and a 4% decline in BMR per decade after the age of 50 [93, 94]. Muscle loss and subsequent reduction in metabolic rate contribute to obesity that accompanies the aging process.

Several studies identify protein as a key nutrient for aging adults [2, 95]. Low protein intake is linked to a decrease in physical ability in aging adults [96]. However, protein intake greater than the dietary guidelines may prevent sarcopenia [96], help maintain BMR [3], improve bone health [4–7], and improve cardiovascular function [8–10]. These benefits of increasing protein in the diet may improve function and quality of life in healthy older adults, as well as improve the ability of older patients to recover from disease and trauma [91].

Currently, the dietary recommendations for protein intake are the same for all healthy adults above the age of 19. However, experts in the field of protein and aging recommend a protein intake between 1.2 and 2.0 g kg<sup>−</sup><sup>1</sup> day<sup>−</sup><sup>1</sup> or higher for elderly adults [91, 95, 97]. The RDA of 0.8 g kg<sup>−</sup><sup>1</sup> day<sup>−</sup><sup>1</sup> is well below these recommendations and reflects a value at the lowest end of the AMDR. It is estimated that 38% of adult men and 41% of adult women have dietary protein intakes below the RDA [16, 44].

Both protein amount and source are important to consider when recommending protein intake to older adults [34, 35]. There are three important aspects to take into consideration when recommending a protein source: (1) the characteristics of the specific protein, such as the amount of essential amino acids (EAA); (2) the food matrix in which the protein is consumed, for example, as part of a beverage or a complete meal; and (3) the characteristics of the individuals consuming the food, including health status, physiological status, and energy balance [34]. In addition, the difference in digestibility and bioavailability of a protein can impact the quantity of protein that needs to be ingested to meet metabolic needs; this is especially important in older adults since gastric motility and nutrient absorption decrease with age. The speed of protein digestion and absorption of amino acids

from the gut can influence whole-body protein building [36]. Proteins with differing amino acid profiles exhibit different digestion and absorption rates [36, 38, 98]. Amino acid availability depends directly on both the quality and quantity of the dietary protein [98].

### **8.3 Dietary protein and gut health**

Over the past 15 years, the gut microbiome has received increased attention regarding its role in impacting overall health [99]. Interestingly, it has been shown to influence diseases associated with metabolic health [100]. The intestinal mucosa houses nearly a trillion microorganisms, and the plasticity of this environment is highly reactive to changes in diet [101]. For instance, the gut becomes an active site for protein and amino acid metabolism prior to absorption. Following enzymatic denaturation by intestinal proteases, amino acids can become fermented into various metabolites which include short-chain fatty acids and ammonia [102]. The acute microbial response and long-term adaptation associated with dietary habits have become an important area of research.

As gut assay methodologies improve, researchers have identified associations between microbial populations and their metabolite concentrations in response to dietary patterns. For instance, in vitro and human models demonstrate a potential negative link between animal protein intake and protein fermentation end products such as ammonia and trimethylamine-N-oxide [103, 104]. However, favorable outcomes associated with animal- and plant-based protein sources have been observed. For example, ingestion of both whey [105] and pea protein [106] has been shown to increase favorable gut bacterial species such as *Bifidobacterium*. In addition, supplementation with pea protein intake has been shown to increase the production of short-chain fatty acids, an important energy substrate utilized by enterocytes [106].

## **9. Conclusions**

There is sufficient evidence that protein intake higher than the current dietary recommendations is beneficial for most healthy individuals throughout the life cycle. However, benefits of dietary protein depend on the quality, the quantity, and the timing of protein intake. Although health benefits of dietary protein have been well-established for older adults, more research is needed to determine the health benefits of increased dietary protein intake through each state of life.

## **Acknowledgements**

This work was supported by a grant to J.I.B. and E.B. from the Arkansas Biosciences Institute.

**125**

**Author details**

\*, Elisabet Børsheim2,3,4, Brittany R. Allman2,3 and Samuel Walker1

1 Department of Food Science, Center for Human Nutrition, University of

2 Arkansas Children's Research Institute, Little Rock, AR, United States

3 Department of Pediatrics, University of Arkansas for Medical Sciences,

4 Department of Geriatrics, University of Arkansas for Medical Sciences,

© 2020 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

Arkansas, Fayetteville, Arkansas, United States

\*Address all correspondence to: baum@uark.edu

provided the original work is properly cited.

Little Rock, AR, United States

Little Rock, AR, United States

Jamie I. Baum1

*Health Benefits of Dietary Protein throughout the Life Cycle*

*DOI: http://dx.doi.org/10.5772/intechopen.91404*

### **Conflict of interest**

The authors have no conflicts of interest to declare.

*Health Benefits of Dietary Protein throughout the Life Cycle DOI: http://dx.doi.org/10.5772/intechopen.91404*

*The Health Benefits of Foods - Current Knowledge and Further Development*

dietary protein [98].

**9. Conclusions**

**Acknowledgements**

Biosciences Institute.

**Conflict of interest**

**8.3 Dietary protein and gut health**

become an important area of research.

from the gut can influence whole-body protein building [36]. Proteins with differing amino acid profiles exhibit different digestion and absorption rates [36, 38, 98]. Amino acid availability depends directly on both the quality and quantity of the

Over the past 15 years, the gut microbiome has received increased attention regarding its role in impacting overall health [99]. Interestingly, it has been shown to influence diseases associated with metabolic health [100]. The intestinal mucosa houses nearly a trillion microorganisms, and the plasticity of this environment is highly reactive to changes in diet [101]. For instance, the gut becomes an active site for protein and amino acid metabolism prior to absorption. Following enzymatic denaturation by intestinal proteases, amino acids can become fermented into various metabolites which include short-chain fatty acids and ammonia [102]. The acute microbial response and long-term adaptation associated with dietary habits have

As gut assay methodologies improve, researchers have identified associations between microbial populations and their metabolite concentrations in response to dietary patterns. For instance, in vitro and human models demonstrate a potential negative link between animal protein intake and protein fermentation end products such as ammonia and trimethylamine-N-oxide [103, 104]. However, favorable outcomes associated with animal- and plant-based protein sources have been observed. For example, ingestion of both whey [105] and pea protein [106] has been shown to increase favorable gut bacterial species such as *Bifidobacterium*. In addition, supplementation with pea protein intake has been shown to increase the production of short-chain fatty acids, an important energy substrate utilized by enterocytes [106].

There is sufficient evidence that protein intake higher than the current dietary recommendations is beneficial for most healthy individuals throughout the life cycle. However, benefits of dietary protein depend on the quality, the quantity, and the timing of protein intake. Although health benefits of dietary protein have been well-established for older adults, more research is needed to determine the health

benefits of increased dietary protein intake through each state of life.

The authors have no conflicts of interest to declare.

This work was supported by a grant to J.I.B. and E.B. from the Arkansas

**124**

## **Author details**

Jamie I. Baum1 \*, Elisabet Børsheim2,3,4, Brittany R. Allman2,3 and Samuel Walker1

1 Department of Food Science, Center for Human Nutrition, University of Arkansas, Fayetteville, Arkansas, United States

2 Arkansas Children's Research Institute, Little Rock, AR, United States

3 Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, United States

4 Department of Geriatrics, University of Arkansas for Medical Sciences, Little Rock, AR, United States

\*Address all correspondence to: baum@uark.edu

© 2020 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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[24] Jen V, Karagounis LG, Jaddoe VWV, Franco OH, Voortman T. Dietary protein intake in school-age children and detailed measures of body composition: The Generation R Study. International Journal of Obesity. 2018;**42**:1715-1723

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**Chapter 6**

Choices

**Abstract**

consumer choices.

**1. Introduction**

serving size

*and Tamara Bucher*

Nutrients for Money: The

Relationship between Portion Size,

Overweight and obesity are major risk factors for chronic disease and in the past 40–50 years portion sizes of offered foods, especially energy-dense, nutrient poor varieties, have dramatically increased along with global rates of overweight and obesity. Studies have shown that offering larger portion sizes result in increased food intake, known as the 'portion size effect'. This is likely due to consumption norms, the expected satiation and satiety of larger portions and the effect of unit bias. In addition, inconsistencies between serving sizes on nutrition information labelling compared to national dietary guidelines, makes it difficult for consumers to estimate and select appropriate portion sizes. Consumers find larger portion sizes more appealing due to their perceived value for money however, the nutritive value of the food is most often not acknowledged. Nutrient profiling models, which classify foods based on their nutrient density per unit cost may help consumers make healthier food choices. This narrative review aims to provide an overview of the portion size effect and discusses the application of nutrient profile score-based labels as a means of promoting nutrient density as value for money to influence

**Keywords:** energy density, nutrient density, portion size, portion size effect,

Globally, non-communicable diseases are the leading cause of mortality and morbidity, contributing to 73% of total deaths and 62% of disability adjustedlife years (DALYs) [1]. Overweight and obesity are a leading risk factor for the development of non-communicable diseases [2]. It is widely accepted that diet is a major contributor to an energy imbalance by which energy intake exceeds energy expenditure over an accumulative period of time, leading to the development of overweight and obesity [3]. Limiting the consumption of energy-dense, nutrient poor foods to manage energy intake is a strategy recommended for regulating body

Nutrient Density and Consumer

*Rebecca L. Haslam, Rachael Taylor, Jaimee Herbert* 

## **Chapter 6**
