**5. Conclusion**

The results of our analysis show that diabetes mellitus type 2 has a negative effect on the vitality of rat male reproductive cells. We may conclude that the disease affects sperm motility, viability, and DNA stability negatively. The disease increases the production of superoxide radicals and the concentration of malondialdehyde and also affects the mitochondrial activity of spermatozoa. However, diabetes mellitus type 2 has no effect on the acrosome integrity. At the same time, we observed visible differences in the experimental group of ZDF rats suffering from diabetes mellitus type 2 in combination with obesity in all evaluated parameters. However, differences were also observed in the experimental group of ZDN rats who developed type 2 diabetes mellitus but did not have obesity.

## **Acknowledgements**

This research was created with financial support of the projects APVV-15-0544 and VEGA 1/0314/19.

**103**

**Author details**

, Mária Chomová<sup>2</sup>

Comenius University in Bratislava, Slovakia

provided the original work is properly cited.

Slovak University of Agriculture in Nitra, Slovakia

\*Address all correspondence to: evina.tvrda@gmail.com

, Oľga Uličná3

1 Department of Animal Physiology, Faculty of Biotechnology and Food Sciences,

2 Faculty of Medicine, Institute of Medical Chemistry and Clinical Biochemistry,

3 Third Intern Clinic, Faculty of Medicine, Comenius University in Bratislava,

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

and Eva Tvrdá1

\*

Filip Benko1

Slovakia

*ZDF Rats: A Suitable Model to Study Male Reproductive Dysfunction in Diabetes Mellitus…*

*DOI: http://dx.doi.org/10.5772/intechopen.89939*
