**2.2.1 Fatty acid methyl ester analysis (FAMEs)**

FAMEs analysis were carried out after performing alkaline treatment obtained by dissolving the oil (0.05 g) in n-hexane (1 mL) and adding a solution of potassium hydroxide (1 mL; 2 N) in methanol (Christie, 1998). FAMEs were analyzed by gas chromatography by mean of a Shimadzu 17A chromatograph equipped with detector flame ionization and a capillary column. Peaks were identified by comparing their retention times with those of authentic reference compounds.

The fatty acid composition was expressed as relative percentages of each fatty acid calculated considering the internal normalization of the chromatographic peak area.
