**Effect of Seed-Placed Ammonium Sulfate and Monoammonium Phosphate on Germination, Emergence and Early Plant Biomass Production of Brassicae Oilseed Crops**

P. Qian1, R. Urton1, J. J. Schoenau1, T. King1, C. Fatteicher1 and C. Grant2 *1University of Saskatchewan, Saskatoon, SK, 2Agriculture and Agri-Food Canada, Brandon, MB, Canada* 

#### **1. Introduction**

52 Oilseeds

Walkelyn, P.J.; Wan, P.J. (2006). Solvent extraction to obtain edible oil products, In: *Handbook* 

Ward, J. A. Processing high oil content seeds in continuous screw presses. *Journal of the American Oil Chemists´ Society*, Vol. 53 (1976), pp. 261-264, ISSN 1558-9331. Weiss, E. A. (1983). Oilseed processing and products, In: *Oilseed crops*, 528-596, ISBN 0-632-

Willems, P.; Kuipers, N. J. M; De Haan, A. B. Hydraulic pressing of oilseeds: Experimental

Williams, M.A. (2005). Recovery of oils and fats from oilseeds and fatty materials, In: *Bailey´s* 

<http://www.ifc.org/ifcext/enviro.nsf/AttachmentsByTitle/gui\_vegoil\_WB/\$FIL

CRC Press.

05259-7, London: Longman.

Vol. 89 (2008), pp. 8-16, ISSN 0260-8774.

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E/vegoil\_PPAH.pdf>. Acessed 01 august 2011.

New Jersey: John Wiley & Sons, Inc.

*of Functional Lipids*, C.C. Akoh (Ed), 89-131, ISBN 978-0-8493-2162-7, Boca Raton:

determination and modeling of yield and pressing rates. *Journal of Food Engineering*,

*Industrial Oil and Fat products*, F. Shahidi (Ed), Vol. 5, 99-189, ISBN 0-471-38552-2,

Seed-placed fertilization, in which fertilizer is placed in the soil in the same furrow as the seed at the time of planting is a common approach to supplying crop nutrients, as this gives newly emerged seedlings early access to nutrients. This placement strategy is noted to be effective for phosphorus fertilizer due to its low mobility in the soil in early spring (Miller et al., 1971; Harapiak, 2006). In the prairies of Canada, cool soil temperatures in the spring at seeding can especially restrict early root growth and access to phosphorus. Therefore it is important for annual crops to be able to access P early in their growth cycle by placing P fertilizer where the roots of the seedling can readily access it, such as in the seed-row. This is the reason why seed-placed phosphorus fertilization normally achieves better response than surface-applied with incorporation. Lauzon and Miller (1997) reported that early season corn and soybean shoot-P concentrations are increased with increasing soil test P and were increased with seed-placed P regardless of soil test P level. However, the germination and emergence of crop seeds can be reduced by seed-placed phosphate fertilizer, as some crop seeds are especially sensitive to the salt effect of fertilizers (Qian and Schoenau, 2010).

In western Canada, canola is a major crop and approximately 4.5 million hectares of agricultural land are under *Brassica* oilseed crop production (Malhi et al., 2007). Canolaquality *B. juncea*, *B. carinata*, and oilseed *Camelina sativa* are also being developed as alternative oilseed crops that are better adapted to areas with periods of hot, dry conditions in western Canada. Tolerance of *Brassica* crops to seed-row application of nutrients is low when compared to many other crops, and emergence differences have been observed between open-pollinated and hybrid cultivars (Brandt et al., 2007). Differences have also been noted in the tolerance and responsiveness of yellow- and black-seeded canola cultivars to seed-placed P (Grant, 2008). Qian and Schoenau (2010) reported that canola seed, in general, is sensitive when the seed-placed rate of P is above 30 kg P2O5 ha-1. The development and adoption of maximum safe rates of fertilizer with seed that avoid significant reductions in crop emergence is important for achieving maximum benefit from the fertilizer by producers.

Effect of Seed-Placed Ammonium Sulfate and Monoammonium Phosphate

on Germination, Emergence and Early Plant Biomass Production of Brassicae Oilseed Crops 55

 Available/Extractable Soil pH Organic C Texture NO3 -N P K SO4-S % ――――――mgkg-1―――――

Haverhill 8.0 2.3 loam 45.7 12.3 693 24.0

After collection, the soil was mixed thoroughly in a soil mixer and stored in field-moist condition before use. For measuring basic soil properties, a sample was collected from the mixed soil, and then air-dried, crushed, and passed through a 2-mm sieve and stored at room temperature. Texture was estimated by hand. Electrical conductivity (EC) and pH were measured using 1:1 soil:water suspension. Organic C was measured using an

Species and Type Herbicide Year of Maturity

 5440 HYB LL 2007 Mid-Late 5525 OP CL 2009 Mid-Late 45H26 HYB RR 2006 Mid v1037\* HYB RR 2007 Early-Mid v1040\* HYB RR 2010 Mid 74P00 OP LL 2006 Early-Mid

 Dahinda OP Conv. 2004 Late Xceed 8571 OP CL 2008 Early

ACS- C7 SYN-OP Conv. 2001 Early

Calena OP Conv. N.A. Early

 *B. carinata* OP Conv. N.A. Late

Cultivar system release

Table 2. Selected *Brassica* species/cultivars used in this study.

\*-specialty oil; HYB-Hybrid; OP-open-pollinated; SYN-synthetic; Conv.-conventional

H.E.A.R\* OP RR

LL-Liberty Link; RR-Roundup Ready; CL-Clearfield

*B. napus* 

*B. juncea* 

*B. rapa* 

*Camelina sativa* 

Table 1. Some characteristics of the soil used for seed-placed S and P evaluation.

Brassica crops like canola have higher demand for S than cereal crops. Canola requires about twice the amount of sulfur as cereal crops do, and canola frequently responds to fertilizer S addition in the drier rain-fed cropping areas of the prairie provinces of Canada, where no-till cropping systems are predominant (Canola Council of Canada 2010). A balance in availability of N, S and P for canola is important for both canola yield and quality (Janzen and Bettany, 1984; Jackson, 2000; Karamanos et al., 2005, Qian and Schoenau, 2007). In Canada, the main S fertilizer used is ammonium sulphate (21-0-0-24). Ammonium sulphate, both as prills and as fines, has become a popular source of N and S fertilizer for canola growers. Given the increased prevalence and availability of ammonium sulfate originating as by-products of industrial processes such as flue gas scrubbing, it seems likely that ammonium sulfate will be cost-effective and its use is likely to increase in the future. However, to date there has been little attention given to the tolerance of Brassica crops like canola to seed-row placed ammonium sulfate, both alone and in combination with starter phosphorus. Also, ammonium sulfate has a higher salt index than monoammonium phosphate and can produce significant amounts of free ammonia, leading to the possibility of both osmotic damage and direct ammonia toxicity at high application rates (Follett et al., 1981). Therefore, providing guidelines for maximum safe rates of fertilizer P and S with the seed is essential for achieving maximum benefit from seed-row placement of fertilizer. Current guidelines state that the amount of S that can be safely placed with the seed of canola as ammonium sulfate should be based on N guidelines, and no specific information is provided on tolerance and response to combinations of ammonium sulfate and starter monoammonium phosphate. Nevertheless, existing guidelines were developed some time ago based on seeding equipment with high disturbance and high seed-bed utilization. The trend today is towards low disturbance opener systems. Growers have questioned how much ammonium sulfate can be safely placed in the seed-row along with P as a starter blend for canola, especially with low soil disturbance opener configurations currently used. The objectives of this chapter are to: (1) determine the effects of seed-placed ammonium sulfate (AS) and monoammonium phosphate (MAP) fertilizer applied at different rates on seedling germination and emergence as well as early season dry matter yield under controlled-environmental conditions; and (2) determine if *Brassica* oilseed crops/cultivars differ in their response to seed placed S and P fertilizer. To fully achieve these objectives, experimental works were conducted as described below, and results and conclusions are drawn.

#### **2. Methods and materials**

#### **2.1 Soil, fertilizer and crop seeds**

The soil selected for the study is a Brown Chernozem (US classification: Aridic Haploboroll), loamy textured soil from a long-term alfalfa field (legal location: SW31, Township 20, Range 3, W of 3rd Meridian) in southwestern Saskatchewan, Canada. The soil represents a common soil type (Haverhill association) found in the southern prairies of Saskatchewan. As there was no history of herbicide application, there was no concern with herbicide residue carryover that could affect germination. The soil was collected from the 0-15 cm depth in the fall of 2010 from a field that has been in continuous alfalfa for ten years. The main soil characteristics and nutrient levels are shown in Table 1.

Brassica crops like canola have higher demand for S than cereal crops. Canola requires about twice the amount of sulfur as cereal crops do, and canola frequently responds to fertilizer S addition in the drier rain-fed cropping areas of the prairie provinces of Canada, where no-till cropping systems are predominant (Canola Council of Canada 2010). A balance in availability of N, S and P for canola is important for both canola yield and quality (Janzen and Bettany, 1984; Jackson, 2000; Karamanos et al., 2005, Qian and Schoenau, 2007). In Canada, the main S fertilizer used is ammonium sulphate (21-0-0-24). Ammonium sulphate, both as prills and as fines, has become a popular source of N and S fertilizer for canola growers. Given the increased prevalence and availability of ammonium sulfate originating as by-products of industrial processes such as flue gas scrubbing, it seems likely that ammonium sulfate will be cost-effective and its use is likely to increase in the future. However, to date there has been little attention given to the tolerance of Brassica crops like canola to seed-row placed ammonium sulfate, both alone and in combination with starter phosphorus. Also, ammonium sulfate has a higher salt index than monoammonium phosphate and can produce significant amounts of free ammonia, leading to the possibility of both osmotic damage and direct ammonia toxicity at high application rates (Follett et al., 1981). Therefore, providing guidelines for maximum safe rates of fertilizer P and S with the seed is essential for achieving maximum benefit from seed-row placement of fertilizer. Current guidelines state that the amount of S that can be safely placed with the seed of canola as ammonium sulfate should be based on N guidelines, and no specific information is provided on tolerance and response to combinations of ammonium sulfate and starter monoammonium phosphate. Nevertheless, existing guidelines were developed some time ago based on seeding equipment with high disturbance and high seed-bed utilization. The trend today is towards low disturbance opener systems. Growers have questioned how much ammonium sulfate can be safely placed in the seed-row along with P as a starter blend for canola, especially with low soil disturbance opener configurations currently used. The objectives of this chapter are to: (1) determine the effects of seed-placed ammonium sulfate (AS) and monoammonium phosphate (MAP) fertilizer applied at different rates on seedling germination and emergence as well as early season dry matter yield under controlled-environmental conditions; and (2) determine if *Brassica* oilseed crops/cultivars differ in their response to seed placed S and P fertilizer. To fully achieve these objectives, experimental works were conducted as described below, and results and conclusions are

The soil selected for the study is a Brown Chernozem (US classification: Aridic Haploboroll), loamy textured soil from a long-term alfalfa field (legal location: SW31, Township 20, Range 3, W of 3rd Meridian) in southwestern Saskatchewan, Canada. The soil represents a common soil type (Haverhill association) found in the southern prairies of Saskatchewan. As there was no history of herbicide application, there was no concern with herbicide residue carryover that could affect germination. The soil was collected from the 0-15 cm depth in the fall of 2010 from a field that has been in continuous alfalfa for ten years. The main soil

drawn.

**2. Methods and materials** 

**2.1 Soil, fertilizer and crop seeds** 

characteristics and nutrient levels are shown in Table 1.


Table 1. Some characteristics of the soil used for seed-placed S and P evaluation.

After collection, the soil was mixed thoroughly in a soil mixer and stored in field-moist condition before use. For measuring basic soil properties, a sample was collected from the mixed soil, and then air-dried, crushed, and passed through a 2-mm sieve and stored at room temperature. Texture was estimated by hand. Electrical conductivity (EC) and pH were measured using 1:1 soil:water suspension. Organic C was measured using an


\*-specialty oil; HYB-Hybrid; OP-open-pollinated; SYN-synthetic; Conv.-conventional LL-Liberty Link; RR-Roundup Ready; CL-Clearfield

Table 2. Selected *Brassica* species/cultivars used in this study.

Effect of Seed-Placed Ammonium Sulfate and Monoammonium Phosphate

**2.3 Statistical analysis** 

**3. Results and discussion** 

the rates of 40 kg S ha-1 and above (Table 3).

playing a major role in this case (Follett et al., 1981).

**emergence and early season biomass** 

more sensitive to seed-place fertilizer than corn or cereals.

**season biomass** 

on Germination, Emergence and Early Plant Biomass Production of Brassicae Oilseed Crops 57

Main and interaction effects of *Brassica* cultivars and ammonium sulfate, alone and in combination with monoammonium phosphate, were determined from analysis of variance (ANOVA) using the GLM procedure in SAS (SAS Institute, 2008). Least significant difference

**3.1 Effect of seed-row placed ammonium sulfate on brassicae emergence and early** 

Generally, there was no significant difference in percent emergence up to 20-30 kg S ha -1 when ammonium sulfate was applied alone with the exception of *Camelina sativa. Camelina sativa* appeared more sensitive to AS placed in the seed-row than the *Brassica* crops (Table 3). At rates of 30 kg S ha-1, only *Invigor 5440* and *B. Juncea Dahinda* showed inhibition effect. When application rate approached 40 kg S ha-1, the majority of cultivars were affected. The cultivars *HEAR, 5525 Clearfield* and *74P00LL* were the least sensitive to seed-row placement of AS, possibly reflecting greater seed vigor. In general, percentage emergence that dropped below 80% was observed in some *Brassica* cultivars at rates of 30 kg S ha-1, and more so at

High oleic, low linolenic (HOLL) (*v1037*; *v1040*) were also less sensitive to seed-row placement of AS; however, they were not as resistant as high erucic acid rapeseed (H.E.A.R). The number of days to first seedling emergence increased with increasing S rate. Time to seedling emergence from seeding was approximately 5-7 d. Emergence was generally 1 to 2 d longer in the high S rate treatments. There was a close relationship between seed size and seed coat color and a decrease in percent emergence, with the yellow-seeded and small seeded cultivars slightly more prone to reduced emergence with seed-placed AS. In most cases, the larger the seed size, the better the vigour. The more vigour, the better the seed/seedling is able to cope with early stresses and survive (Canada Canola Council 2005). High rates of AS reduced the seedling emergence, early seedling growth and increased the time to maximum emergence of seeds. There was an increase in the number of abnormal seedlings observed in the higher S treatments due to seedling injury, which can be attributed to the salt effect of the fertilizer

Seedling biomass yield (mg / pot) at 14 days of growth was not significantly affected up to rates of 20 – 30 kg S ha-1 (Table 4). At higher rates in which significant emergence reduction was observed, seedling biomass was also reduced. As Hall (2007) indicated, canola is much

**3.2 Effect of combinations of ammonium sulfate and monoammonium phosphate on** 

Addition of 15 to 30 kg P2O5 ha-1 along with the S application led to more injury represented by reduced seed germination/emergence and early growth of seedlings, than S alone at corresponding rates. Canola usually shows injury response to MAP alone at rates of 30 kg P2O5 ha-1 and higher (Qian and Schoenau 2010). However, decreases from addition of the

(LSD 0.05) was used to determine significant differences between treatment means.

automated combustion LECO carbon analyzer. Soil available P and K were extracted by modified Kelowna method (Qian et al., 1994). Soil available N was calculated as the sum of NO3-N + NH4-N. Both forms of inorganic N were extracted with 2 M KCl (Keeney and Nelson, 1982) and measured by automated colorimetry. Soil available S was extracted with 0.001 M CaCl2 solution and measured by automated colormetry (Qian et al., 1992). The fertilizer phosphorus source used was conventional commercial fertilizer grade granular monoammonium phosphate MAP (12-51-0). The sulfate source used was fertilizer grade prilled ammonium sulfate (NH4)2SO4 (21-0-0-24). The *Brassica* species/cultivars selected for this study are listed in Table 2. Among the 12 selected cultivars, four different categories of canola were included: open pollinated (OP) and hybrid *napus* (also termed "Argentine" canola) (HYB), *rapa* (also termed "Polish" canola), and *juncea* (sometimes termed "mustard canola"). Although not currently grown on a large commercial scale, *B. carinata* and *C. sativa* are currently under development as new oilseed crops with similar attributes to canola and were therefore included in the study as well.

#### **2.2 Laboratory study**

Plants were grown in plastic trays (52cm x 26cm x 6cm) containing 5.4 kg of uniformly mixed, air-dried soil at 20 degrees C. The soil in each tray was levelled to a height of 5cm over the individual rows and packed. Six 20cm x 2.50cm x 1.25cm seed-rows were created in the trays using a seeding tool. The crops were seeded using a seed quantity per unit area in the row that is equivalent to a seeding rate of approximately 10 kg ha¯¹. The seeding tool used creates a seed bed utilization of approximately 15%, in which 15% of the total seed bed area is used for placement of seed and fertilizer in rows. This seed bed utilization is typical of the wide row spacing, narrow opener seeding tool configurations commonly used for seeding oilseeds in the northern Great Plains today. Sixteen seeds were seeded in each row at a uniform depth of 1.25cm.

The fertilizer was passed through a 2mm sieve to provide uniform granule size and then spread uniformly down the seed-row with the seed. During germination, trays were kept under constant light and regularly watered to maintain soil moisture at 100% water holding capacity for the first two days and ensure germination, and then reduced to 80% of field capacity to maintain soil moisture for seedling growth. Emergence counts were taken every two days after seeding until 14 days after seeding (DAS) when plant counts were constant and no additional emergence was observed. Plants were harvested 14 DAS. Plant biomass samples were washed in deionized water after cutting at the soil surface and oven-dried at 45°C for 3 d to a constant weight.

Six treatments consisting of an unfertilized control and five rates of seed-placed S (10, 20, 30, 40 and 50 kg S ha-1), applied as ammonium sulfate (AS 21-0-0-24) were applied in combination with three rates of seed-placed P205 (0, 15 and 30 kg P2O5 ha-1), applied as monoammonium phosphate (MAP 12-51-0).

The *B*. species/cultivars were designated as main plots, S rates as subplots and P rates as sub-subplots within the trays and were arranged in a randomized complete block design with four replications. Nitrogen was applied at 10, 20, 30, 40, 50 kg N ha-1with the ammonium sulfate alone, and with 15 kg P2O5 ha-1 as well as 30 kg P2O5 ha-1 in the seed-row.
