**Table 1.**

*List of primers used in this study.*

*Right Heart Adaptation to Left Ventricular STEMI in Rats DOI: http://dx.doi.org/10.5772/intechopen.84868*

reverse transcriptase (200 U/μg; Invitrogen) and oligo dTs (Roche, Mannheim, Germany) as primers. Reverse transcriptase reactions were performed for 60 min at 37°C. Real-time PCR was performed using the Icycler IQ detection system (Bio-Rad, Munich, Germany) in combination with IQ SYBR Green real-time supermix (Bio-Rad). A complete list of all primers used in this study is given in **Table 1**. Data are normalized to hypoxanthine phosphoribosyltransferase (HPRT) expression that was used as a house-keeping gene in this study. Preliminary experiments with β2 microglobulin, which was alternatively considered as house-keeping gene, revealed similar results but higher variability. The relative change in expression was quantified by the ΔΔC method [10].
