2. Peptide synthesis

They are two methods of peptide synthesis, namely


Liquid-phase peptide synthesis is the classical method that is slow and labour intensive in which separate peptides are synthesized and then coupled together to create larger peptides. In this method chemical group is used to protect the Cterminus of the first amino acid.

Solid-phase peptide synthesis is the most common method where the Cterminus of the first amino acid is coupled to an activated solid support, such as polystyrene or polyacrylamide. This method is advantageous as the resin acts as the Determination of Substrate Specificity of the Purified Novel Plant Cysteine Protease Solanain… DOI: http://dx.doi.org/10.5772/intechopen.90184

Figure 3. Peptide bonds created with peptide coupling agents.

C-terminal protecting group and it is a rapid method to separate the growing peptide product from the different reaction mixtures during synthesis.

These two ways are combined in an exceedingly method known as native chemical tying. LifeTein's customary amide synthesis method uses the solid part. The liquid-phase approach is employed for the synthesis of short peptides, like diand tripeptides, and C-terminally changed peptides, like accelerator substrates (Figure 3).
