*4.2.5 Insulin*

Encouraging results have been found in the use of insulin for induction of estrus in animals either alone or in combination [21–23]. The recommended dose is 0.25 IU/kg body weight subcutaneously for 3–5 days. Promising results for management of anestrus in cattle have been observed with the use of GnRH or eCG pretreated with insulin [22, 23] and buffaloes [21, 24]. Insulin enhances the follicular growth in true anestrus buffalo which is a prerequisite for GnRH to be effective [24].
