Exploitation of Food Waste

Chapter 5

Abstract

Extraction, Characterization, and

By-products originating from agricultural and food processing are considerable disposal problem for the industry because these waste streams emerge in huge quantities and often have direct impact on the economy and environmental pollution. However, at the same time, it constitute a rich but yet underutilized source of valuable components, which may find application as ingredients in the food and non-food industries. As a result, numerous projects are currently directed toward the utilization of agricultural and food processing by-products such as animal-based (skin, bone, flesh, and internal organs) and plant-based biomaterials (fruit peels and seeds, rice bran, and etc.). In the present work, we would like to focus on the potentialities and the current research of the compounds and extracts deriving from agro-industrial disposable wastes in the food-related utilization. The potential of selected by-products as a source of bioactive/functional compounds in terms of emerging and conventional techniques for extraction, characterization, biological activity monitoring and application of the extracts or isolated compounds as functional food ingredients or bio-based materials for food packaging are highlighted. Considering environmental effect and economic loss, agricultural and food processing by-products should be utilized in various innovative processes in the

Keywords: by-products, fish industry, film, food packaging, fruit and vegetables,

Thailand is situated in the heart of the Southeast Asian mainland, fertile, tropical country with monsoonal climate that reinforce agriculture both in land and off land [1]. Besides, Thailand owes high agricultural output to it alluvial soils and topography, plentiful water supplies, which allows several plantings a year. Agriculture has long been the mainstay of the Thai economy, with abundant natural resources, and the majority of the Thai population engages in agricultural practices. Agricultural products in Thailand can be classified into four types: (1) crops, such as rice (number one export item of the country), tapioca, natural rubber, and pineapple; (2) forestry and timber products (40% of the area in Thailand has been designated as forests); (3) fishery products, including freshwater and saltwater fisheries; and

Application of Agricultural and

Food Processing By-Products

Saroat Rawdkuen and Sunantha Ketnawa

cause of beneficial product derivation.

1. Introduction

75

gelatin, legume seeds, oilseed, rice, utilization

#### Chapter 5

## Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

Saroat Rawdkuen and Sunantha Ketnawa

#### Abstract

By-products originating from agricultural and food processing are considerable disposal problem for the industry because these waste streams emerge in huge quantities and often have direct impact on the economy and environmental pollution. However, at the same time, it constitute a rich but yet underutilized source of valuable components, which may find application as ingredients in the food and non-food industries. As a result, numerous projects are currently directed toward the utilization of agricultural and food processing by-products such as animal-based (skin, bone, flesh, and internal organs) and plant-based biomaterials (fruit peels and seeds, rice bran, and etc.). In the present work, we would like to focus on the potentialities and the current research of the compounds and extracts deriving from agro-industrial disposable wastes in the food-related utilization. The potential of selected by-products as a source of bioactive/functional compounds in terms of emerging and conventional techniques for extraction, characterization, biological activity monitoring and application of the extracts or isolated compounds as functional food ingredients or bio-based materials for food packaging are highlighted. Considering environmental effect and economic loss, agricultural and food processing by-products should be utilized in various innovative processes in the cause of beneficial product derivation.

Keywords: by-products, fish industry, film, food packaging, fruit and vegetables, gelatin, legume seeds, oilseed, rice, utilization

#### 1. Introduction

Thailand is situated in the heart of the Southeast Asian mainland, fertile, tropical country with monsoonal climate that reinforce agriculture both in land and off land [1]. Besides, Thailand owes high agricultural output to it alluvial soils and topography, plentiful water supplies, which allows several plantings a year. Agriculture has long been the mainstay of the Thai economy, with abundant natural resources, and the majority of the Thai population engages in agricultural practices. Agricultural products in Thailand can be classified into four types: (1) crops, such as rice (number one export item of the country), tapioca, natural rubber, and pineapple; (2) forestry and timber products (40% of the area in Thailand has been designated as forests); (3) fishery products, including freshwater and saltwater fisheries; and

(4) livestock products such as chicken, eggs, and pork, which are also in high demand in the domestic market and exported [2, 3].

food manufacturing by-products into useful materials as a means of achieving goal of sustainable development. Hence, considerable efforts in the valorization of agricultural and food processing residues have been made with the purpose of minimizing the amounts of by-products, reducing the environment pollution, and increasing sustainability of these by-products. This section reviews by-products from two main food processing industries such as animal-based biomaterials (fish skin, bone, flesh, and internal organs) and plant-based biomaterials (pineapple, mango, longan, tea, sacha inchi, oilseeds, legumes, rice, etc.) that have a potential to be produced as gelatin, seasoning powder, calcium powder, film, protein concentrate, isolate and hydrolysates, bioactive peptides, and others such as protease,

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

Thailand is one of the top fish-producing nations in the world, supplying markets in the USA, Europe, and Asia. Fishery production in Thailand demonstrated a remarkable growth over the last three decades. The total production exceeded 0.7 (freshwater fish) and 3 million tons (marine fish) over the last 5 years. Thailand is now the largest exporter of canned tuna, chilled and frozen shrimp, shrimp products, chilled fish, and prawns. Accounting for 1.5% of various kinds of fishery products for exporting are fresh and frozen prawn, processed shrimp, squid, cuttlefish, fish fillet, and surimi and fish in the form of fresh, chilled-frozen, and

Freshwater fish are widely cultured for commercial exploitation and trade in Thailand. The Mekong giant catfish (Pangasianodon gigas) is one of the economically important farmed fish that has been successfully raised in Northern Thailand, especially in Chiang Rai province [11]. Recently, farmed giant catfish (GC) became an economically important cultured freshwater fish due to their quality attributes (i.e., texture, taste, color, and nutritive values). Morphology of Mekong giant catfish can be seen in Figure 1, and most farm-raised giant catfish are sold raw to restaurants, and in the near future, fish farmers hope to export its meat to other countries, especially in Asia and Europe. It is reported that fish-processing byproducts after filleting account for 75% of the total fish weight [12]. The viscera in some species of fish account for approximately 5–10% of the entire weight of the fish; the viscera percentage tends to increase with the fish body weight [12]. Nile tilapia, Oreochromis niloticus, is popular in freshwater aquaculture. In the global

market, the demand for tilapia (TP) in all forms is increasing rapidly. More by-products have been produced from the expansion of the tilapia processing

An increase in fish consumption has resulted in an increase in fish-processing by-products, and these, in turn, may contribute significantly to environmental pollution if there is no further valorization process. With an increase in fish processing, a large amount of internal organs and by-product are also generated. During the filleting, 50–65% of the body is discarded which are environmental pollutants. More than 60% of these residues, including of the bone/frame (12–17%), viscera (4–12%), heads (18–60%), cut-offs (6%), skin (4–7%), and roe (2%) are considered as waste as shown in Table 1 and Figure 2. The waste is used in the production of fish meal, and in a very few cases, it is collected for the production of animal feeds; more frequently it is simply discarded. The utilization of these

tyrosinase, antioxidant, and antimicrobial agents.

2. Fish industry and by-products

DOI: http://dx.doi.org/10.5772/intechopen.89289

processed food [11].

industry [13].

77

2.1 Fish-processing by-products

Thailand is one of the world's leading exporters of rice and also a major exporter of shrimp. The major trading partners with Thailand in agricultural products, ranked by export value, are as follows: (1) rice (Nigeria, China, Japan, and the USA); (2) natural rubber (Benin, Malaysia, the United Kingdom, and Japan); (3) processed chicken (South Africa, Japan, the Netherlands, Canada, and Taiwan); (4) chilled or frozen shrimp (Cote d'Ivoire, the USA, Germany, and the Republic of Korea); and (5) tapioca products (Ghana, the Republic of Korea, Ireland, the United Kingdom, and Indonesia) [2]. Thailand's food processing industry has developed rapidly and is one of the most developed in Southeast Asia with more than 10,000 food and beverage processing factories. Thailand has a large fresh, frozen, and semi-cooked food industry. In 2017, food exports generated an income of more than US\$26 billion or about 830 billion baht to the country, employing as many as 10.75 million workers in the agricultural and fishery sectors, which is expected to reach US\$27.4 billion for 2019 [4, 5]. According to the Thai government, "Thailand ranks among the top of the world's food producing countries in several food categories". Thailand is therefore considered one of the world's important food exporting countries. The fast-growing demand for food by the world's population bodes well for the limitless expansion of the consumer market [2, 5, 6]. According to data supplied by the Trade and Economic Information Center, the Ministry of Commerce, the main groups of food export are as follows: (1) frozen seafood group, canned seafood, and processed seafood; (2) fresh chicken and products; (3) other food groups (including ready-to-eat food and semi-cooked food); (4) halal group or food and food products for Muslims, prepared as prescribed by Muslim law; (5) frozen vegetable and fruit group, canned vegetables and fruits, canned fruit in syrup, processed vegetables and fruits, and vegetable pickles [2].

In addition, Thailand government looks set to revitalize the Kitchen of the World campaign, a scheme to accelerate Thailand's plan to become a major world food exporter, as it aims to boost food exports to contribute >6% of the country's GDP. Initiated 15 years ago, the Kitchen of the World campaign aims not only to merely accelerate Thailand's plan to become a major world food exporter but also spearhead the export of products from the One Tambon One Product (OTOP) scheme. Thailand also approved the Strategic Framework for Food Management in Thailand (2017–2021), which aims to produce enough food to sustain domestic demand, support access to adequate food at all times, improve food quality, reduce food waste and use food correctly, promote sustainable food production, and support the development of food security and nutrition [7].

From the above information, number of production and exportation, and Thai government policy, we can understandably assume that a large amount of food materials as by-products, which are generated along the chain of food production and transformation, are thrown into the environment as consumption demand is growing. Not only Thailand concern, but the global food losses, by-products and wastes are also hot issue that needs to be taken into consideration. The highest percentage of by-products is found in fruits and vegetables, plus roots and tubers (45%), followed by fish and seafood (35%), and oilseed, meat, and dairy (20%), respectively [8]. According to Ezejiofor et al. [9], by-products from food manufacturing make up to 30–60% of the product that is utilized for human consumption and animal feeding. Most of the by-products commonly contain proteins, carbohydrates, and lipids, which are promising sources of value-added substances that can be extracted and utilized as a starting material for enzyme, gelatin, collagen, bioactive compounds, for example, enzymes and antioxidants extractions [10]. Current trends in the world as well as in Thailand are to recover and utilize the Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289

food manufacturing by-products into useful materials as a means of achieving goal of sustainable development. Hence, considerable efforts in the valorization of agricultural and food processing residues have been made with the purpose of minimizing the amounts of by-products, reducing the environment pollution, and increasing sustainability of these by-products. This section reviews by-products from two main food processing industries such as animal-based biomaterials (fish skin, bone, flesh, and internal organs) and plant-based biomaterials (pineapple, mango, longan, tea, sacha inchi, oilseeds, legumes, rice, etc.) that have a potential to be produced as gelatin, seasoning powder, calcium powder, film, protein concentrate, isolate and hydrolysates, bioactive peptides, and others such as protease, tyrosinase, antioxidant, and antimicrobial agents.

#### 2. Fish industry and by-products

(4) livestock products such as chicken, eggs, and pork, which are also in high demand

of shrimp. The major trading partners with Thailand in agricultural products, ranked by export value, are as follows: (1) rice (Nigeria, China, Japan, and the USA); (2) natural rubber (Benin, Malaysia, the United Kingdom, and Japan); (3) processed chicken (South Africa, Japan, the Netherlands, Canada, and Taiwan); (4) chilled or frozen shrimp (Cote d'Ivoire, the USA, Germany, and the Republic of Korea); and (5) tapioca products (Ghana, the Republic of Korea, Ireland, the United Kingdom, and Indonesia) [2]. Thailand's food processing industry has developed rapidly and is one of the most developed in Southeast Asia with more than 10,000 food and beverage processing factories. Thailand has a large fresh, frozen, and semi-cooked food industry. In 2017, food exports generated an income of more than US\$26 billion or about 830 billion baht to the country, employing as many as 10.75 million workers in the agricultural and fishery sectors, which is expected to reach US\$27.4 billion for 2019 [4, 5]. According to the Thai government, "Thailand ranks among the top of the world's food producing countries in several food categories". Thailand is therefore considered one of the world's important food exporting countries. The fast-growing demand for food by the world's population bodes well for the limitless expansion of the consumer market [2, 5, 6]. According to data supplied by the Trade and Economic Information Center, the Ministry of Commerce, the main groups of food export are as follows: (1) frozen seafood group, canned seafood, and processed seafood; (2) fresh chicken and products; (3) other food groups (including ready-to-eat food and semi-cooked food); (4) halal group or food and food products for Muslims, prepared as prescribed by Muslim law; (5) frozen vegetable and fruit group, canned vegetables and fruits, canned fruit in syrup,

Thailand is one of the world's leading exporters of rice and also a major exporter

in the domestic market and exported [2, 3].

Food Preservation and Waste Exploitation

processed vegetables and fruits, and vegetable pickles [2].

port the development of food security and nutrition [7].

76

In addition, Thailand government looks set to revitalize the Kitchen of the World campaign, a scheme to accelerate Thailand's plan to become a major world food exporter, as it aims to boost food exports to contribute >6% of the country's GDP. Initiated 15 years ago, the Kitchen of the World campaign aims not only to merely accelerate Thailand's plan to become a major world food exporter but also spearhead the export of products from the One Tambon One Product (OTOP) scheme. Thailand also approved the Strategic Framework for Food Management in Thailand (2017–2021), which aims to produce enough food to sustain domestic demand, support access to adequate food at all times, improve food quality, reduce food waste and use food correctly, promote sustainable food production, and sup-

From the above information, number of production and exportation, and Thai government policy, we can understandably assume that a large amount of food materials as by-products, which are generated along the chain of food production and transformation, are thrown into the environment as consumption demand is growing. Not only Thailand concern, but the global food losses, by-products and wastes are also hot issue that needs to be taken into consideration. The highest percentage of by-products is found in fruits and vegetables, plus roots and tubers (45%), followed by fish and seafood (35%), and oilseed, meat, and dairy (20%),

respectively [8]. According to Ezejiofor et al. [9], by-products from food manufacturing make up to 30–60% of the product that is utilized for human consumption and animal feeding. Most of the by-products commonly contain proteins, carbohydrates, and lipids, which are promising sources of value-added substances that can be extracted and utilized as a starting material for enzyme, gelatin, collagen, bioactive compounds, for example, enzymes and antioxidants extractions [10]. Current trends in the world as well as in Thailand are to recover and utilize the

Thailand is one of the top fish-producing nations in the world, supplying markets in the USA, Europe, and Asia. Fishery production in Thailand demonstrated a remarkable growth over the last three decades. The total production exceeded 0.7 (freshwater fish) and 3 million tons (marine fish) over the last 5 years. Thailand is now the largest exporter of canned tuna, chilled and frozen shrimp, shrimp products, chilled fish, and prawns. Accounting for 1.5% of various kinds of fishery products for exporting are fresh and frozen prawn, processed shrimp, squid, cuttlefish, fish fillet, and surimi and fish in the form of fresh, chilled-frozen, and processed food [11].

Freshwater fish are widely cultured for commercial exploitation and trade in Thailand. The Mekong giant catfish (Pangasianodon gigas) is one of the economically important farmed fish that has been successfully raised in Northern Thailand, especially in Chiang Rai province [11]. Recently, farmed giant catfish (GC) became an economically important cultured freshwater fish due to their quality attributes (i.e., texture, taste, color, and nutritive values). Morphology of Mekong giant catfish can be seen in Figure 1, and most farm-raised giant catfish are sold raw to restaurants, and in the near future, fish farmers hope to export its meat to other countries, especially in Asia and Europe. It is reported that fish-processing byproducts after filleting account for 75% of the total fish weight [12]. The viscera in some species of fish account for approximately 5–10% of the entire weight of the fish; the viscera percentage tends to increase with the fish body weight [12]. Nile tilapia, Oreochromis niloticus, is popular in freshwater aquaculture. In the global market, the demand for tilapia (TP) in all forms is increasing rapidly. More by-products have been produced from the expansion of the tilapia processing industry [13].

#### 2.1 Fish-processing by-products

An increase in fish consumption has resulted in an increase in fish-processing by-products, and these, in turn, may contribute significantly to environmental pollution if there is no further valorization process. With an increase in fish processing, a large amount of internal organs and by-product are also generated. During the filleting, 50–65% of the body is discarded which are environmental pollutants. More than 60% of these residues, including of the bone/frame (12–17%), viscera (4–12%), heads (18–60%), cut-offs (6%), skin (4–7%), and roe (2%) are considered as waste as shown in Table 1 and Figure 2. The waste is used in the production of fish meal, and in a very few cases, it is collected for the production of animal feeds; more frequently it is simply discarded. The utilization of these

angiotensin-I-converting enzyme (ACE) inhibitory activity [20, 21] or the extraction of carotenoprotein from shrimp waste [22]. Fish bone is another by-product that can serve as a raw material for the production of high value-added compounds that can be used in various sectors including agrochemical, biomedical, food, and pharmaceutical industries [23]. Fish bone is considered as one of the potential biological sources to produce calcium phosphates [23, 24]. Fish bone can be applied

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

The growing consumer demand for healthy fish products has led to a thriving fish-processing industry worldwide. The fish-processing industry generates significant amounts of by-products and waste as illustrated in Table 1 and Figure 2 and tends to increase with fish body weight and consumption [26]. It is estimated that 25% of the global fish production is regarded as waste and is discarded or in the best case scenario processed into fish oil, fishmeal fertilizer, fish silage, and animal feed [27, 28]. It is costly to treat these wastes because it contains high content of organic matter. In addition, disposing these wastes may cause severe health and environ-

In general, gelatin is manufactured from the waste generated during animal slaughter and processing, that is, skin and bone. The most abundant gelatin sources are pig skin (46%), bovine hide (29.4%), and pork and cattle bones (23.1%) [29]. For several reasons, there are still serious concerns among the consumers to consume gelatin which is produced from bovine and porcine bones and skins. This is because of some problems such as religious matter and mad cow disease (bovine spongiform encephalopathy (BSE)) [16]. Thus, it lead to many researchers to discover alternative sources such as fish (marine and freshwater) [16, 18, 30] and poultry by-products [31]. As a consequence, there has been increasing interest in other sources, especially in fish skin and bone from fishery processing by-products.

Gelatin was extracted from the skin of farmed giant catfish with a yield of 19.50 g/100 g skin sample on wet weight basis (Figure 3). The gelatin had high protein (89.1 g/100 g) but low fat (0.75 g/100 g) content and contained a high number of imino acids (proline and hydroxyproline) (211 residues per 1000

to boost nutritional characteristics in seasoning powder [25].

Wastes and by-products of Nile tilapia and giant catfish. Source: Vannabun et al. [14].

mental issues as well as increase the cost of the fish industry.

2.2 Utilization of fishery by-products

DOI: http://dx.doi.org/10.5772/intechopen.89289

Figure 2.

79

2.3 Gelatin and collagen from fish skin

#### Figure 1. Farm and morphology of Mekong giant catfish.


#### Table 1.

Fish-processing by-products of freshwater fish including giant catfish, striped catfish, and Nile tilapia.

by-products could make more profit to the producer and ease environmental problems. Besides, they will be benefit for the producer to gain more profit from their materials instead of discard. These by-products contain a significant amount of protein that could be converted into useful substances and used for food formulation and bioactive ingredients.

In order to upgrade the by-products of the fish industry, various systematic studies were made to fulfill the best utilization of fish-processing by-products. Fish skin, in particular, is a rich source of collagen and gelatin, and these by-products still contain a significant amount of protein-rich material [15–18]. Using proteases from fish viscera in combined with commercial enzymes can be an alternative way to reduce the enzymes costs [12, 14, 19]. Alternatively, digestive enzymes from shrimp or fish viscera can be recovered and be successfully used as processing aids, that is, for the production of gelatin hydrolysate with antioxidant activities and/or

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289

Figure 2. Wastes and by-products of Nile tilapia and giant catfish. Source: Vannabun et al. [14].

angiotensin-I-converting enzyme (ACE) inhibitory activity [20, 21] or the extraction of carotenoprotein from shrimp waste [22]. Fish bone is another by-product that can serve as a raw material for the production of high value-added compounds that can be used in various sectors including agrochemical, biomedical, food, and pharmaceutical industries [23]. Fish bone is considered as one of the potential biological sources to produce calcium phosphates [23, 24]. Fish bone can be applied to boost nutritional characteristics in seasoning powder [25].

#### 2.2 Utilization of fishery by-products

The growing consumer demand for healthy fish products has led to a thriving fish-processing industry worldwide. The fish-processing industry generates significant amounts of by-products and waste as illustrated in Table 1 and Figure 2 and tends to increase with fish body weight and consumption [26]. It is estimated that 25% of the global fish production is regarded as waste and is discarded or in the best case scenario processed into fish oil, fishmeal fertilizer, fish silage, and animal feed [27, 28]. It is costly to treat these wastes because it contains high content of organic matter. In addition, disposing these wastes may cause severe health and environmental issues as well as increase the cost of the fish industry.

#### 2.3 Gelatin and collagen from fish skin

In general, gelatin is manufactured from the waste generated during animal slaughter and processing, that is, skin and bone. The most abundant gelatin sources are pig skin (46%), bovine hide (29.4%), and pork and cattle bones (23.1%) [29]. For several reasons, there are still serious concerns among the consumers to consume gelatin which is produced from bovine and porcine bones and skins. This is because of some problems such as religious matter and mad cow disease (bovine spongiform encephalopathy (BSE)) [16]. Thus, it lead to many researchers to discover alternative sources such as fish (marine and freshwater) [16, 18, 30] and poultry by-products [31]. As a consequence, there has been increasing interest in other sources, especially in fish skin and bone from fishery processing by-products. Gelatin was extracted from the skin of farmed giant catfish with a yield of 19.50 g/100 g skin sample on wet weight basis (Figure 3). The gelatin had high protein (89.1 g/100 g) but low fat (0.75 g/100 g) content and contained a high number of imino acids (proline and hydroxyproline) (211 residues per 1000

by-products could make more profit to the producer and ease environmental problems. Besides, they will be benefit for the producer to gain more profit from their materials instead of discard. These by-products contain a significant amount of protein that could be converted into useful substances and used for food formula-

Fish-processing by-products of freshwater fish including giant catfish, striped catfish, and Nile tilapia.

Whole fish 15–17 (kg) 4.1–4.6 (kg) 0.8–1 kg Fillet skinned 50–52 42–45 4.6–6 Skin 4–6 5–7 4.5–6 Viscera 4–5 10–12 5–8 Head 25–27 18–20 52–60 Bone 12–15 15–17 — Scale — — 2–3 Yield (%) 50–52 48–59 64–77 By-products (%) 45–53 48–56 64–77

Giant catfish Striped catfish Nile Tilapia

Component Yield (%)

Farm and morphology of Mekong giant catfish.

Food Preservation and Waste Exploitation

In order to upgrade the by-products of the fish industry, various systematic studies were made to fulfill the best utilization of fish-processing by-products. Fish skin, in particular, is a rich source of collagen and gelatin, and these by-products still contain a significant amount of protein-rich material [15–18]. Using proteases from fish viscera in combined with commercial enzymes can be an alternative way to reduce the enzymes costs [12, 14, 19]. Alternatively, digestive enzymes from shrimp or fish viscera can be recovered and be successfully used as processing aids, that is, for the production of gelatin hydrolysate with antioxidant activities and/or

tion and bioactive ingredients.

Source: Vannabun et al. [14].

Table 1.

78

Figure 1.

residues). Hydroxyproline accounted for 87 residues per 1000 residues. Giant catfish (GC) skin gelatin had a slightly different amino acid composition than calf (BF) skin gelatin. Gelatin was extracted from the skin of GC and tilapia (TP) at a yield of 19.50 and 23.34%, respectively. Gelatin from GC skin was lower in ash content (0.33 g/100 g). GC skin gelatin was rich in nonpolar amino acid glycine (35.9 g/100 g), alanine (10.6 g/100 g), and proline (12.4 g/100 g) [30].

Microstructures in transverse section photomicrographs of both GC and TP skin clearly projected the arrangement of collagenous bundles in each fish skin (Figure 4). TP showed a small bundle with the same ordered pattern, while in GC, the alternate arrangement was observed with a large bundle. The gaps between collagenous bundles represent the compact structure of the component in the skin. This particular characteristic affected the extractability of the collagen and the gelatin in the structure. The relative thickness of the two dermal layers depends on the presence or absence of scales, and it varies with the fish species. In general, the skin of animal is composed of collagen as a main compound. GC skin showed the large collagenous bundles with ordered arrangement when compared with the TP skin, while TP skin showed more compact and small bundles of collagenous components. These results cause different extraction yields of gelatin. The compact arrangement of the bundle effect to the strength and hardness of the fish skin [16].

Chemical compositions of both the fish skin and the gelatins are also presented in Table 2. Proteins are the major compound ranging 84–88% (wet wt.). Other components are moisture, ash, and lipid. For the skin of fresh fish, moisture was the

Microstructure of giant catfish skin (A) and tilapia (B). LM examined at 400 magnifications and SEM magnification of 500 at acceleration voltage of 10 kV. Source: Rawdkuen et al. [16] and Sai-Ut et al. [18].

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

DOI: http://dx.doi.org/10.5772/intechopen.89289

For the functional properties, the results showed that GC and TP are comparable to BF. Protein patterns by using SDS-PAGE showed high band intensity for the αand β-components in TP, while BF showed the lowest band intensity of the major

Giant catfish Tilapia Bovine Giant catfish Tilapia

major constituent (53–67%). Protein found in GC was higher than TP.

Constituents Gelatin Fish skin

Sources: Rawdkuen et al. [16] and Sai-Ut et al. [18].

Yield (%) 19.50 1.87 23.34 2.49 — —— Moisture 3.39 0.43 8.49 0.82 11.38 0.72 53.8 5.3 67.7 0.5 Protein 85.27 0.68 84.28 1.04 88.33 1.10 43.0 0.9 30.6 0.9 Lipid 1.24 0.15 0.45 0.18 0.09 0.01 1.6 0.5 1.1 0.1 Ash 0.17 0.03 0.15 0.09 1.30 0.22 0.3 0.1 2.1 0.4

Gelatin yield and proximate composition of fish skin and gelatin from farmed giant catfish, Nile tilapia, and

commercial beef skin gelatin. Values are given as mean SD from triplicate determinations.

Figure 4.

Table 2.

81

#### Figure 3.

Extraction of collagen and gelatin form farmed giant catfish skin. Sources: Rawdkuen et al. [16] and Sai-Ut et al. [18]. Different lowercase letters in the bar chart indicate significant difference (p < 0.05).

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289

#### Figure 4.

residues). Hydroxyproline accounted for 87 residues per 1000 residues. Giant catfish (GC) skin gelatin had a slightly different amino acid composition than calf (BF) skin gelatin. Gelatin was extracted from the skin of GC and tilapia (TP) at a yield of 19.50 and 23.34%, respectively. Gelatin from GC skin was lower in ash content

Microstructures in transverse section photomicrographs of both GC and TP skin clearly projected the arrangement of collagenous bundles in each fish skin (Figure 4). TP showed a small bundle with the same ordered pattern, while in GC, the alternate arrangement was observed with a large bundle. The gaps between collagenous bundles represent the compact structure of the component in the skin. This particular characteristic affected the extractability of the collagen and the gelatin in the structure. The relative thickness of the two dermal layers depends on the presence or absence of scales, and it varies with the fish species. In general, the skin of animal is composed of collagen as a main compound. GC skin showed the large collagenous bundles with ordered arrangement when compared with the TP skin, while TP skin showed more compact and small bundles of collagenous components. These results cause different extraction yields of gelatin. The compact arrangement of the bundle effect to the strength and hardness of the fish skin [16].

Extraction of collagen and gelatin form farmed giant catfish skin. Sources: Rawdkuen et al. [16] and Sai-Ut

et al. [18]. Different lowercase letters in the bar chart indicate significant difference (p < 0.05).

(0.33 g/100 g). GC skin gelatin was rich in nonpolar amino acid glycine (35.9 g/100 g), alanine (10.6 g/100 g), and proline (12.4 g/100 g) [30].

Food Preservation and Waste Exploitation

Figure 3.

80

Microstructure of giant catfish skin (A) and tilapia (B). LM examined at 400 magnifications and SEM magnification of 500 at acceleration voltage of 10 kV. Source: Rawdkuen et al. [16] and Sai-Ut et al. [18].

Chemical compositions of both the fish skin and the gelatins are also presented in Table 2. Proteins are the major compound ranging 84–88% (wet wt.). Other components are moisture, ash, and lipid. For the skin of fresh fish, moisture was the major constituent (53–67%). Protein found in GC was higher than TP.

For the functional properties, the results showed that GC and TP are comparable to BF. Protein patterns by using SDS-PAGE showed high band intensity for the αand β-components in TP, while BF showed the lowest band intensity of the major


#### Table 2.

Gelatin yield and proximate composition of fish skin and gelatin from farmed giant catfish, Nile tilapia, and commercial beef skin gelatin. Values are given as mean SD from triplicate determinations.

component (Figure 5). From the study, it can be concluded that the GC and TP are a prospective source for gelatin production with desirable functionalities. In addition, fish skin gelatin could be more effectively and widely used in food ingredient industries [16].

#### 2.4 Gelatin application

#### 2.4.1 Antimicrobial film

Edible films prepared from different types of biomaterials such as proteins, polysaccharide, lipids, or their blends exhibit different film properties. Among all of these materials, proteins are considered to provide desirable mechanical, gas barrier and transparency properties, as well as high nutrition [32]. Rawdkuen et al. [15] prepared edible films from the gelatin of farmed giant catfish skin (GC), bovine bone gelatin (BB), and their combination (50:50) as depicted in Figure 6. The total color difference (ΔE) value of BB films was lower than CG and GC films, respectively. The GC films showed significantly lower values of L and a but highest in b value (p < 0.05). When compared with the low-density polyethylene film, GC and BB films showed the highest and lowest total of color differences, respectively. BB film had a lighter color and more yellowness than other films where the highest redness was observed in GC films. The GC films showed the most compact, smooth, and continuous surface without porous structures, which corresponds to the greater properties of films [15].

#### 2.4.2 Antimicrobial and antioxidant films

Some studies regarding to cooperation some natural extracted into protein-based film was summarized in Table 3. Natural extracts such as catechin-Kradon, catechin-lysozyme, nisin-catechin, and longan seed extract were used as active ingredients to delay lipid oxidation or retard microbial growth in soybean oil or fresh meat and fish slice. In vitro antibacterial of films incorporated with nisin and/

#### Figure 5.

SDS-PAGE patterns of gelatin from farmed fish skins and commercial beef skin gelatin under nonreducing conditions. Source: Rawdkuen et al. [16].

or catechin was investigated by Kaewprachu et al. [33]. Interestingly, gelatin films with nisin and catechin retarded lipid oxidation and microbial growth: the time to reach a total viable count of 10<sup>7</sup> CFUg<sup>1</sup> of meat was extended from 1 to 4 days as

Surface morphology of gelatin films from bovine bone gelatin, giant catfish skin gelatin and their combination of

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

300, and 500 ppm

100 g 0.06 g/ 100 g

100 g

0.3, and 0.5% (w/v)

0, 0.125, 0.25, and 0.5% (w/v)

0, 3, 6, 9, and 12 mg/ml

(v/v)

Soybean oil 50, 100,

Minced pork 0.12 g/

Minced pork 0.5 g/

Neem extract Minced beef 0, 0.1,

S. cerevisiae, S. aureus, E. coli, and L. innocua

Vibrio parahaemolyticus

Tuna slice 0.9%

Antioxidant and antimicrobial activity of protein-based films incorporated with antioxidant or

Sample tested Loading Observation References

Prevent effect on lipid oxidation 30 days of storage

Retarded lipid oxidation and lower microbial growth rates

Inhibition of lipid oxidation and lower microbial growth rates than those of PVC

> Improve mechanical, physicochemical, and antimicrobial properties

Total volatile base nitrogen, peroxide value, and TBARS decreased and lowest growth of psychrophilic bacteria

Delay oxidative reactions Putsakum

Antimicrobial activity Kaewprachu

Vichasilp et al. [37] and Sai-Ut et al. [38]

Kaewprachu et al. [33]

Kaewprachu et al. [34]

et al. [35]

Rawdkuen et al. [39]

et al. [40]

Kaewprachu et al. [36]

bovine bone:giant catfish (50:50). Source: Rawdkuen et al. [15].

Figure 6.

Fish myofibrillar protein

Table 3.

83

antimicrobial agents.

Protein Antioxidant/

Gelatin Longan seed

antimicrobial agents

DOI: http://dx.doi.org/10.5772/intechopen.89289

extract

Nisin and/or catechin

Catechinlysozyme combination (1:1)

Catechinlysozyme combination

Catechin and Kradon extract

Catechin and Kradon combination extracts

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289

#### Figure 6.

component (Figure 5). From the study, it can be concluded that the GC and TP are a prospective source for gelatin production with desirable functionalities. In addition, fish skin gelatin could be more effectively and widely used in food ingredient

Edible films prepared from different types of biomaterials such as proteins, polysaccharide, lipids, or their blends exhibit different film properties. Among all of these materials, proteins are considered to provide desirable mechanical, gas barrier and transparency properties, as well as high nutrition [32]. Rawdkuen et al. [15] prepared edible films from the gelatin of farmed giant catfish skin (GC), bovine bone gelatin (BB), and their combination (50:50) as depicted in Figure 6. The total color difference (ΔE) value of BB films was lower than CG and GC films, respectively. The GC films showed significantly lower values of L and a but highest in b value (p < 0.05). When compared with the low-density polyethylene film, GC and BB films showed the highest and lowest total of color differences, respectively. BB film had a lighter color and more yellowness than other films where the highest redness was observed in GC films. The GC films showed the most compact, smooth, and continuous surface without porous structures, which corresponds to the greater

Some studies regarding to cooperation some natural extracted into protein-based

film was summarized in Table 3. Natural extracts such as catechin-Kradon, catechin-lysozyme, nisin-catechin, and longan seed extract were used as active ingredients to delay lipid oxidation or retard microbial growth in soybean oil or fresh meat and fish slice. In vitro antibacterial of films incorporated with nisin and/

SDS-PAGE patterns of gelatin from farmed fish skins and commercial beef skin gelatin under nonreducing

industries [16].

2.4 Gelatin application

Food Preservation and Waste Exploitation

2.4.1 Antimicrobial film

properties of films [15].

Figure 5.

82

conditions. Source: Rawdkuen et al. [16].

2.4.2 Antimicrobial and antioxidant films

Surface morphology of gelatin films from bovine bone gelatin, giant catfish skin gelatin and their combination of bovine bone:giant catfish (50:50). Source: Rawdkuen et al. [15].


#### Table 3.

Antioxidant and antimicrobial activity of protein-based films incorporated with antioxidant or antimicrobial agents.

or catechin was investigated by Kaewprachu et al. [33]. Interestingly, gelatin films with nisin and catechin retarded lipid oxidation and microbial growth: the time to reach a total viable count of 10<sup>7</sup> CFUg<sup>1</sup> of meat was extended from 1 to 4 days as illustrated in Figure 7. Catechin-lysozyme incorporated gelatin film (CLGF) also showed positive results in preserving the quality and maintaining shelf life of minced pork when compared with that of PVC film [34]. It was found that sample wrapped with CLGF showed less weight loss, less discoloration, and low TBARS content than that wrapped with PVC. Microbial numbers in the sample wrapped with the CLGF (TPC count 4.15 log CFU/g; yeast and mold 2.99 log CFU/g) were lower than those observed in the PVC [34]. The properties of gelatin films incorporated with neem extract (NE; Azadirachta indica) as well as catechin-Kradon extract were determined as depicted in Figure 7. It was found that gelatin film containing NE is potentially a promising film that could help delay oxidative reactions over a 7-day period in a refrigerated storage (4 1°C) [35].

suppressed Shelf life extension from 4 to 8 days was obtained in FMP sample. FMP showed high efficiency in both antimicrobial and antioxidant activities [36]. Gelatin film incorporated with longan seed extract or BHT could be used as a tool to prolong the shelf life of oily foods [37]. Antioxidant activity of the films incorporated with longan seed extract markedly increased when the storage time increased. LS- or BHT-incorporated films showed the preventive effect on lipid oxidation of soybean oil during storage for 30 days. Longan seed extract film (500 ppm) provided the highest efficacy for lipid oxidation retardation as evidenced by lower

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

Apart from film application, gelatin from food processing by-products can be applied to make clarification in juice or protection from syneresis of yogurt. In application as guava juice clarifier study by Widyasari and Rawdkuen [31], the addition of gelatin at different concentrations showed different results, where the highest value of turbidity was observed from tilapia gelatin at concentration of 1% (w/v) (382 FTU). Furthermore, the lowest turbidity value is purchased at 0.16% (w/v) from chicken feet gelatin (108 FTU). On the other hand, the syneresis of yogurt after centrifugation at 4°C ranged from 0.0 to 71.49%, where the lowest syneresis index was obtained at 1% (w/v) level of the added gelatin from chicken

Fish bone weighs as high as 52–60% of fish body weight. Proximate composition of giant catfish bone was shown in Table 4. Fish bones can serve as a raw material for the production of high value-added compounds that can be used in various sectors including agrochemical, biomedical, food, and pharmaceutical industries [23]. Those bones can serve as a promising source of calcium. The bone is composed of 34–36% calcium, based on total ash content, and is also rich in collagen and chondroitin. Additionally, fish bone is the source of hydroxyapatite (HA) known as

biocalcium (BC) powder, which is derived from bio-mineralized or a naturally

Permpoon et al. [25] worked to develop food seasoning powder supplemented with fish bone as depicted in Figure 8. Different concentrations of fish bone powder (0–3%, w/w) were added in those recipes with and without monosodium glutamate (MSG). The content of minerals was shown in Table 5. The highest score of overall acceptance was 6.40 in the sample supplemented with 0.5% fish bone powder containing MSG. High calcium, phosphorus, and sodium were predominant mineral

Composition Percentage Moisture 6.79 0.09 Protein 28.14 0.10 Fat 8.61 0.38 Minerals 50.09 0.19

<sup>3</sup>)6(OH)2. Those bones could be converted to

conjugated diene values.

DOI: http://dx.doi.org/10.5772/intechopen.89289

2.4.3 Other applications

feet and tilapia gelatin.

2.5 Fish bone

(Ca2+)10<sup>x</sup>(H3O<sup>+</sup>

produced resource [24].

Source: Terzioğlu et al. [23].

Proximate composition of giant catfish bone.

Table 4.

85

)2<sup>x</sup>(PO4

content in the sample supplemented with fish bone [25].

Besides, catechin-Kradon extract (Careya sphaerica Roxb.) was incorporated with fish myofibrillar protein films (FMP) [36]. It was found that decreases in the redness index for the tuna slices were coincidental with increases in metmyoglobin content stored in a refrigerator (4 1°C) for 10 days (Figure 7). Moreover, the developed film delayed peroxide value, TBARS, and metmyoglobin contents in Bluefin tuna as well as total volatile base nitrogen and psychrophilic bacteria were

#### Figure 7.

Quality attribute of meat wrapped with myofibrillar protein film incorporated with natural extract. Sources: Kaewprachu et al. [33], Kaewprachu et al. [34], Kaewprachu et al. [36], and Putsakum et al. [35]. A, C, E, and G represent minced beef wrapped with polyvinyl chloride, PVC and B.D,F, and H represent minced beef wrapped with gelatin film containing 0.3% neem extract, for 0,3,5, and 7 day, respectively.

#### Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289

suppressed Shelf life extension from 4 to 8 days was obtained in FMP sample. FMP showed high efficiency in both antimicrobial and antioxidant activities [36]. Gelatin film incorporated with longan seed extract or BHT could be used as a tool to prolong the shelf life of oily foods [37]. Antioxidant activity of the films incorporated with longan seed extract markedly increased when the storage time increased. LS- or BHT-incorporated films showed the preventive effect on lipid oxidation of soybean oil during storage for 30 days. Longan seed extract film (500 ppm) provided the highest efficacy for lipid oxidation retardation as evidenced by lower conjugated diene values.

#### 2.4.3 Other applications

illustrated in Figure 7. Catechin-lysozyme incorporated gelatin film (CLGF) also showed positive results in preserving the quality and maintaining shelf life of minced pork when compared with that of PVC film [34]. It was found that sample wrapped with CLGF showed less weight loss, less discoloration, and low TBARS content than that wrapped with PVC. Microbial numbers in the sample wrapped with the CLGF (TPC count 4.15 log CFU/g; yeast and mold 2.99 log CFU/g) were lower than those observed in the PVC [34]. The properties of gelatin films incorporated with neem extract (NE; Azadirachta indica) as well as catechin-Kradon extract were determined as depicted in Figure 7. It was found that gelatin film containing NE is potentially a promising film that could help delay oxidative reac-

Besides, catechin-Kradon extract (Careya sphaerica Roxb.) was incorporated with fish myofibrillar protein films (FMP) [36]. It was found that decreases in the redness index for the tuna slices were coincidental with increases in metmyoglobin content stored in a refrigerator (4 1°C) for 10 days (Figure 7). Moreover, the developed film delayed peroxide value, TBARS, and metmyoglobin contents in Bluefin tuna as well as total volatile base nitrogen and psychrophilic bacteria were

Quality attribute of meat wrapped with myofibrillar protein film incorporated with natural extract. Sources: Kaewprachu et al. [33], Kaewprachu et al. [34], Kaewprachu et al. [36], and Putsakum et al. [35]. A, C, E, and G represent minced beef wrapped with polyvinyl chloride, PVC and B.D,F, and H represent minced beef

wrapped with gelatin film containing 0.3% neem extract, for 0,3,5, and 7 day, respectively.

tions over a 7-day period in a refrigerated storage (4 1°C) [35].

Food Preservation and Waste Exploitation

Figure 7.

84

Apart from film application, gelatin from food processing by-products can be applied to make clarification in juice or protection from syneresis of yogurt. In application as guava juice clarifier study by Widyasari and Rawdkuen [31], the addition of gelatin at different concentrations showed different results, where the highest value of turbidity was observed from tilapia gelatin at concentration of 1% (w/v) (382 FTU). Furthermore, the lowest turbidity value is purchased at 0.16% (w/v) from chicken feet gelatin (108 FTU). On the other hand, the syneresis of yogurt after centrifugation at 4°C ranged from 0.0 to 71.49%, where the lowest syneresis index was obtained at 1% (w/v) level of the added gelatin from chicken feet and tilapia gelatin.

#### 2.5 Fish bone

Fish bone weighs as high as 52–60% of fish body weight. Proximate composition of giant catfish bone was shown in Table 4. Fish bones can serve as a raw material for the production of high value-added compounds that can be used in various sectors including agrochemical, biomedical, food, and pharmaceutical industries [23]. Those bones can serve as a promising source of calcium. The bone is composed of 34–36% calcium, based on total ash content, and is also rich in collagen and chondroitin. Additionally, fish bone is the source of hydroxyapatite (HA) known as (Ca2+)10<sup>x</sup>(H3O<sup>+</sup> )2<sup>x</sup>(PO4 <sup>3</sup>)6(OH)2. Those bones could be converted to biocalcium (BC) powder, which is derived from bio-mineralized or a naturally produced resource [24].

Permpoon et al. [25] worked to develop food seasoning powder supplemented with fish bone as depicted in Figure 8. Different concentrations of fish bone powder (0–3%, w/w) were added in those recipes with and without monosodium glutamate (MSG). The content of minerals was shown in Table 5. The highest score of overall acceptance was 6.40 in the sample supplemented with 0.5% fish bone powder containing MSG. High calcium, phosphorus, and sodium were predominant mineral content in the sample supplemented with fish bone [25].


#### Table 4. Proximate composition of giant catfish bone.

tuna is considered a promising source for BC production due to its abundance. BC with improved color and odor along with increased solubility in gastrointestinal tract can be used as an alternative calcium supplement to tackle the inadequate

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

The waste portions such as the peel, core, stem, and crown were 29–40, 9–10, 2–5, and 2–4% (w/w), respectively (Figures 9 and 10 and Table 6) [42–45]. From the last decade, the use of plant extracts as a source of bioactive components (phytochemicals) has gained wide attention against synthetic antibiotic drugs. Pineapple peels and cores account for about 40% of the whole fruit, and they are largely wasted after fresh-cut processing. Papaya fruit is the green fruit which is widely used in Thai cuisine and famously in papaya salad. As the consumption increases, large quantities of papaya peels as by-products are left [42]. Usually, the peels are occasionally used for animal feed or disposed, which produce phytopathogens, then cause ecological problems, and pose risks to human health. These wastes are occasionally utilized as fertilizers or animal feed, yet they have very low economic value. Therefore, ways to utilize these residue wastes have become an important focus for research and development, recognizing that a systematic reduction in waste disposal is beneficial both economically and ecologically. Thus, fruit waste extracts are suitable for enhancing the nutritional and antioxidant properties in food, and moreover, they can also be applied in cosmetics and the nutraceutical and pharmaceutical industries [46]. Therefore, the utilization these waste products have become an important task for research and development. Systematically reducing waste and putting it to other uses is profitable both economically and

Peel is the outermost covering of fruits and not commonly consumed. Pineapple and papaya peels are found to have potential uses as raw materials that could be converted into value-added products, especially as sources for bioactive compound extraction. The utilization of fruit peels as a source of bioactive compounds, espe-

The isolation and characterization of bromelain extract (BE) from the wastes of Nang Lae and Phu Lae pineapple cultivars (economical fruits of Chiang Rai province, Thailand) were investigated by Ketnawa et al. [43] and Ketnawa et al. [44].

Peel from agriculture products, pineapple, papaya, and mango (left to right). Source: Ketnawa et al. [42].

intake of dietary calcium.

3. Plant processing by-products

DOI: http://dx.doi.org/10.5772/intechopen.89289

ecologically speaking [37, 38].

cially in proteolytic enzymes extraction means [44, 47].

3.2 Enzyme extraction and application

3.1 Fruit peel

Figure 9.

87

#### Figure 8.

Food seasoning powder supplemented with fish bone. Source: Permpoon et al. [25].


Source: Permpoon et al. [25]

\* Values are represented as mean ± SD (n = 2). Different lowercase letters in the same row indicate significant difference (p < 0.05). \*\*mg/kg

#### Table 5.

Mineral content in food seasoning supplemented with fish bone values which are presented as mean SD (n = 2). Different lowercase letters in the same row indicate significant difference (p < 0.05).

#### 2.5.1 Fish bone as calcium and phosphorus source

Fish bones have high calcium content, and large quantities of this raw material are available as a waste from the fishery industry. Previously, emphasis has been done on producing high-quality products from fish by-products by the use of bacterial proteases [41]. Biocalcium powder from the bones of precooked skipjack tuna was produced and characterized compared with calcined bone (CB) powder. Higher calcium (40.35%) and phosphorous (15.28%) contents were found in the CB powder than BC powder (26.91 and 12.63%, respectively). BC powder had a low abundance of volatile compounds, including aldehydes, alcohols, and ketones. Precooked skipjack tuna bone could be used as a raw material for preparing BC powder, which has a different composition than CB powder. The BC powder was still composed of collagenous protein. BC had a low abundance of odorous compounds, whereas the CB powder had a negligible amount of volatiles. Precooked skipjack

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289

tuna is considered a promising source for BC production due to its abundance. BC with improved color and odor along with increased solubility in gastrointestinal tract can be used as an alternative calcium supplement to tackle the inadequate intake of dietary calcium.

#### 3. Plant processing by-products

The waste portions such as the peel, core, stem, and crown were 29–40, 9–10, 2–5, and 2–4% (w/w), respectively (Figures 9 and 10 and Table 6) [42–45]. From the last decade, the use of plant extracts as a source of bioactive components (phytochemicals) has gained wide attention against synthetic antibiotic drugs. Pineapple peels and cores account for about 40% of the whole fruit, and they are largely wasted after fresh-cut processing. Papaya fruit is the green fruit which is widely used in Thai cuisine and famously in papaya salad. As the consumption increases, large quantities of papaya peels as by-products are left [42]. Usually, the peels are occasionally used for animal feed or disposed, which produce phytopathogens, then cause ecological problems, and pose risks to human health. These wastes are occasionally utilized as fertilizers or animal feed, yet they have very low economic value. Therefore, ways to utilize these residue wastes have become an important focus for research and development, recognizing that a systematic reduction in waste disposal is beneficial both economically and ecologically. Thus, fruit waste extracts are suitable for enhancing the nutritional and antioxidant properties in food, and moreover, they can also be applied in cosmetics and the nutraceutical and pharmaceutical industries [46]. Therefore, the utilization these waste products have become an important task for research and development. Systematically reducing waste and putting it to other uses is profitable both economically and ecologically speaking [37, 38].

#### 3.1 Fruit peel

Peel is the outermost covering of fruits and not commonly consumed. Pineapple and papaya peels are found to have potential uses as raw materials that could be converted into value-added products, especially as sources for bioactive compound extraction. The utilization of fruit peels as a source of bioactive compounds, especially in proteolytic enzymes extraction means [44, 47].

#### 3.2 Enzyme extraction and application

The isolation and characterization of bromelain extract (BE) from the wastes of Nang Lae and Phu Lae pineapple cultivars (economical fruits of Chiang Rai province, Thailand) were investigated by Ketnawa et al. [43] and Ketnawa et al. [44].

#### Figure 9.

Peel from agriculture products, pineapple, papaya, and mango (left to right). Source: Ketnawa et al. [42].

2.5.1 Fish bone as calcium and phosphorus source

Food seasoning powder supplemented with fish bone. Source: Permpoon et al. [25].

Calcium\*\* 94.48 8.65b 1637.50 252.44<sup>a</sup> 120.25 5.02b Potassium 2088.50 41.72a 2115.50 85.56<sup>a</sup> 2122.50 136.47<sup>a</sup> Phosphorus 493.90 23.19<sup>b</sup> 1338.00 0.69<sup>a</sup> 683.70 32.24<sup>b</sup> Magnesium 60.62 2.98<sup>c</sup> 80.38 0.69b 92.52 3.96<sup>a</sup> Sodium 182,550 <sup>6151</sup><sup>a</sup> 185,050 19,728a 171,800 16,687<sup>a</sup>

Values are represented as mean ± SD (n = 2). Different lowercase letters in the same row indicate significant difference (p < 0.05).

Mineral content in food seasoning supplemented with fish bone values which are presented as mean SD

(n = 2). Different lowercase letters in the same row indicate significant difference (p < 0.05).

Component Conditions\*

Food Preservation and Waste Exploitation

Figure 8.

Source: Permpoon et al. [25]

\*

86

\*\*mg/kg

Table 5.

Fish bones have high calcium content, and large quantities of this raw material are available as a waste from the fishery industry. Previously, emphasis has been done on producing high-quality products from fish by-products by the use of bacterial proteases [41]. Biocalcium powder from the bones of precooked skipjack tuna was produced and characterized compared with calcined bone (CB) powder. Higher calcium (40.35%) and phosphorous (15.28%) contents were found in the CB powder than BC powder (26.91 and 12.63%, respectively). BC powder had a low abundance of volatile compounds, including aldehydes, alcohols, and ketones. Precooked skipjack tuna bone could be used as a raw material for preparing BC powder, which has a different composition than CB powder. The BC powder was still composed of collagenous protein. BC had a low abundance of odorous compounds, whereas the CB powder had a negligible amount of volatiles. Precooked skipjack

Control 1% Fish bone Commercial

Bromelain has been used commercially in the food industry, in certain cosmetics, and in dietary supplements. It is used for meat tenderizing, brewing, and baking, as well as for the production of protein hydrolysates [42]. Toughness is one of the most common quality characteristics of meat and can be subdivided into actomyosin toughness and background toughness. The former is attributable to changes in myofibrillar proteins, whereas the latter is attributable to connective tissues. Treatment by proteolytic enzymes is a popular method for meat tenderization. Proteolytic enzymes derived from plants such as papain, bromelain, and ficin have been widely used as meat tenderizers in most parts of the world. Ketnawa and Rawdkuen [45] used bromelain extract obtained from the top phase of an aqueous two-phase system (18% PEG-6000 + 17% MgSO4) to tender the muscle foods with different concentrations of BE (0–20%, (w/w)) for 1 h. A reduction of meat firmness and toughness was observed in all samples when compared to the control. Electrophoretic patterns revealed extensive proteolysis and a reduction in number and intensity of the protein bands in all of the treated samples. The results showed that the bromelain extract could be used as an effective meat tenderizer. In similar study of Ketnawa et al. [43], the effects of the bromelain extract on the protein patterns of beef, chicken, and squid muscles were also determined as depicted in Figure 11. Trichloroacetic acid-soluble peptide content of all the treated muscles increased when the amount of bromelain extract increased. Decrease in myosin

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

DOI: http://dx.doi.org/10.5772/intechopen.89289

SDS-PAGE patterns of muscles treated with bromelain from pineapple peels. BC, CC, and SC, controls without extract: B, beef; C, chicken; and S, squid. The numbers 1 and 2 indicate the amount of extract, 1 and 2 mL, respectively. MHC, myosin heavy chain; AC, actin; TM, tropomyosin; TN-T, troponin-T; and MLC, myosin

Figure 11.

89

light chain. Source: Ketnawa et al. [43].

#### Figure 10.

By-products from pineapple fruits. Source: Ketnawa et al. [44].


#### Table 6.

Proportion of pineapple fruits of Nang Lae and Phu Lae cultivars.

The extract of crown from both cultivars gave the highest proteolytic activity and protein contents, while the extract from the stem exhibited the lowest values. Bromelain is a major enzyme with the MW 28 kDa. This study founded that there is much added value into local Thailand pineapple wastes because of bromelain extraction [44]. Chaiwut et al. [47] have reported that they obtained a substantial extraction of proteases from papaya peels. Tyrosinase inhibitor enzyme was extracted from mango seed kernel [46, 48].

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289

Bromelain has been used commercially in the food industry, in certain cosmetics, and in dietary supplements. It is used for meat tenderizing, brewing, and baking, as well as for the production of protein hydrolysates [42]. Toughness is one of the most common quality characteristics of meat and can be subdivided into actomyosin toughness and background toughness. The former is attributable to changes in myofibrillar proteins, whereas the latter is attributable to connective tissues. Treatment by proteolytic enzymes is a popular method for meat tenderization. Proteolytic enzymes derived from plants such as papain, bromelain, and ficin have been widely used as meat tenderizers in most parts of the world. Ketnawa and Rawdkuen [45] used bromelain extract obtained from the top phase of an aqueous two-phase system (18% PEG-6000 + 17% MgSO4) to tender the muscle foods with different concentrations of BE (0–20%, (w/w)) for 1 h. A reduction of meat firmness and toughness was observed in all samples when compared to the control. Electrophoretic patterns revealed extensive proteolysis and a reduction in number and intensity of the protein bands in all of the treated samples. The results showed that the bromelain extract could be used as an effective meat tenderizer. In similar study of Ketnawa et al. [43], the effects of the bromelain extract on the protein patterns of beef, chicken, and squid muscles were also determined as depicted in Figure 11. Trichloroacetic acid-soluble peptide content of all the treated muscles increased when the amount of bromelain extract increased. Decrease in myosin

#### Figure 11.

SDS-PAGE patterns of muscles treated with bromelain from pineapple peels. BC, CC, and SC, controls without extract: B, beef; C, chicken; and S, squid. The numbers 1 and 2 indicate the amount of extract, 1 and 2 mL, respectively. MHC, myosin heavy chain; AC, actin; TM, tropomyosin; TN-T, troponin-T; and MLC, myosin light chain. Source: Ketnawa et al. [43].

The extract of crown from both cultivars gave the highest proteolytic activity and protein contents, while the extract from the stem exhibited the lowest values. Bromelain is a major enzyme with the MW 28 kDa. This study founded that there is much added value into local Thailand pineapple wastes because of bromelain extraction [44]. Chaiwut et al. [47] have reported that they obtained a substantial extraction of proteases from papaya peels. Tyrosinase inhibitor enzyme was

Part of pineapple Nang Lae Phu Lae

Peel 143.40 30.09 159.86 42.20 Core 44.61 9.36 40.60 10.72 Stem 26.55 5.57 9.26 2.44 Crown 22.48 4.72 10.20 2.69 Flesh 239.86 50.33 158.94 41.95 Total 476.54 100.00 378.86 100.00

Weight (g) % (w/w) Weight (g) % (w/w)

extracted from mango seed kernel [46, 48].

Proportion of pineapple fruits of Nang Lae and Phu Lae cultivars.

By-products from pineapple fruits. Source: Ketnawa et al. [44].

Food Preservation and Waste Exploitation

Figure 10.

Source: Ketnawa et al. [43].

Table 6.

88

heavy chains and actin was observed in all the muscle types when bromelain extract was used [43]. The technology for applying this enzyme is cheaply available and can be exploited at the household or industrial level for tenderizing meat, and it can be used as an alternative to chemical tenderizers or other plant proteases.

#### 3.3 Fruit seed and kernel

Recent studies have shown that fruit's waste parts like mango seed contain a noteworthy amount of bioactive component of therapeutic worth [48, 49]. These biologically active components include mangiferin, flavonoids, catechin, phenolic acids, gallic acid, and gallic acid derivatives as shown in Figure 12. The therapeutic importance of these compounds have evaluated through in-vitro and minimal preclinically, but there is a need for proper preclinical trials and afterward clinical trials for health claims and health benefits. The industrial processing of mangoes produces several million tons of waste from their peels and seeds at various stages that cause for a major disposal problem and effect to the environment. The seed alone makes up about 20% of the whole fruit, with 45–78% of the seed being the seed kernel [49, 50]. During processing of mango, by-products such as peel and kernel are generated.

The major polyphenols present in mangos that act as a source of natural antioxidants (Figure 12) are as follows: mangiferin, catechin, quercetin, kaempferol, cinnamic acids, tanins, etc. The extract of the mango peels and the seed kernels also has a great deal of tyrosinase inhibitor, antioxidant activity, and chelating activity [46, 49, 51].

#### 3.3.1 Bioactive compound extraction

To enhance the efficiency of the extraction process, optimization the extraction parameters: the liquid/solid ratio, the ethanol concentration, and the extraction time are consideration. The study describes the optimization of polyphenol extraction from mango seed kernels by using response surface methodology (RSM) (Figure 13). Sai-Ut et al. [46] applied RSM to optimize the ethanolic extraction of polyphenols from mango peels. Nam-Dokmai peel (NDP) showed significantly higher phenolic content and tyrosinase inhibitor activity than that of the Tong-Dam peel (TDP). The optimal condition that maximized the extraction yields, EPC, and the antioxidant activities for NDP were ethanol proportion of 49%, a temperature of 61°C, and an extraction time of 221 min, whereas the optimized condition that maximized the extraction yields, TPC, and antioxidant activities from mango

Tong-Dam kernel (TDK) was an ethanol concentration of 62%, a temperature of 63°C, and a duration of 150 min with a fixed ratio of 1:30 solid-liquid [48].

Response surface methodology to optimize the extraction of polyphenol compounds from plant wastes. Sources:

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

DOI: http://dx.doi.org/10.5772/intechopen.89289

Sacha inchi is widely utilized as a raw material in the edible oil industry not only in South Africa but also in Asian country. The de-oiled meals contain high amounts of proteins, which makes it highly desirable for industrial use as value-added products. The proximate compositions of sacha inchi used in this study included protein 459, carbohydrate 361, fat 67, ash 59, crude fiber 58, and moisture 53 g/kg (wb). A comparative study of pressed cake made from tea and sacha inchi seeds was performed. Sacha inchi seeds contained the largest amount of protein (62.07%) and tea seeds contained the largest amount of carbohydrates (82.68%) as shown in Figure 14. Lysine, leucine, histidine, and phenylalanine were the main essential amino acids. Protein patterns by using SDS-PAGE showed that the main protein component had MWs of 35–63 for sacha inchi and 11–20 kDa for tea seeds. In addition, it contained glycoprotein with a MW of 35 kDa. Both pressed cakes

3.4 Legume/grain/defatted seed flour/rice bran

Figure 13.

91

Rawdkuen et al. [48] Sai-Ut et al. [46].

Figure 12. Chemical structures of some phenolic compounds found in plant by-products.

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289

#### Figure 13.

heavy chains and actin was observed in all the muscle types when bromelain extract was used [43]. The technology for applying this enzyme is cheaply available and can be exploited at the household or industrial level for tenderizing meat, and it can be

Recent studies have shown that fruit's waste parts like mango seed contain a noteworthy amount of bioactive component of therapeutic worth [48, 49]. These biologically active components include mangiferin, flavonoids, catechin, phenolic acids, gallic acid, and gallic acid derivatives as shown in Figure 12. The therapeutic importance of these compounds have evaluated through in-vitro and minimal preclinically, but there is a need for proper preclinical trials and afterward clinical trials for health claims and health benefits. The industrial processing of mangoes produces several million tons of waste from their peels and seeds at various stages that cause for a major disposal problem and effect to the environment. The seed alone makes up about 20% of the whole fruit, with 45–78% of the seed being the seed kernel [49, 50]. During processing of mango, by-products such as peel and kernel are generated. The major polyphenols present in mangos that act as a source of natural antiox-

idants (Figure 12) are as follows: mangiferin, catechin, quercetin, kaempferol, cinnamic acids, tanins, etc. The extract of the mango peels and the seed kernels also has a great deal of tyrosinase inhibitor, antioxidant activity, and chelating activity

To enhance the efficiency of the extraction process, optimization the extraction parameters: the liquid/solid ratio, the ethanol concentration, and the extraction time are consideration. The study describes the optimization of polyphenol extraction from mango seed kernels by using response surface methodology (RSM) (Figure 13). Sai-Ut et al. [46] applied RSM to optimize the ethanolic extraction of polyphenols from mango peels. Nam-Dokmai peel (NDP) showed significantly higher phenolic content and tyrosinase inhibitor activity than that of the Tong-Dam peel (TDP). The optimal condition that maximized the extraction yields, EPC, and the antioxidant activities for NDP were ethanol proportion of 49%, a temperature of 61°C, and an extraction time of 221 min, whereas the optimized condition that maximized the extraction yields, TPC, and antioxidant activities from mango

used as an alternative to chemical tenderizers or other plant proteases.

3.3 Fruit seed and kernel

Food Preservation and Waste Exploitation

[46, 49, 51].

Figure 12.

90

Chemical structures of some phenolic compounds found in plant by-products.

3.3.1 Bioactive compound extraction

Response surface methodology to optimize the extraction of polyphenol compounds from plant wastes. Sources: Rawdkuen et al. [48] Sai-Ut et al. [46].

Tong-Dam kernel (TDK) was an ethanol concentration of 62%, a temperature of 63°C, and a duration of 150 min with a fixed ratio of 1:30 solid-liquid [48].

#### 3.4 Legume/grain/defatted seed flour/rice bran

Sacha inchi is widely utilized as a raw material in the edible oil industry not only in South Africa but also in Asian country. The de-oiled meals contain high amounts of proteins, which makes it highly desirable for industrial use as value-added products. The proximate compositions of sacha inchi used in this study included protein 459, carbohydrate 361, fat 67, ash 59, crude fiber 58, and moisture 53 g/kg (wb). A comparative study of pressed cake made from tea and sacha inchi seeds was performed. Sacha inchi seeds contained the largest amount of protein (62.07%) and tea seeds contained the largest amount of carbohydrates (82.68%) as shown in Figure 14. Lysine, leucine, histidine, and phenylalanine were the main essential amino acids. Protein patterns by using SDS-PAGE showed that the main protein component had MWs of 35–63 for sacha inchi and 11–20 kDa for tea seeds. In addition, it contained glycoprotein with a MW of 35 kDa. Both pressed cakes

#### Figure 14.

Some utilization of pressed cake from sacha inchi and tea seeds. Source: Rawdkuen et al. [53]. A and B represent Electrophoresis patterns of protein and glycoprotein of pressed-cake from tea and sacha inchi seeds, respectively.

showed good sources for bioactive compounds with high antioxidant activities. Therefore, the chemical properties of the pressed cakes indicate that this byproduct of oil extraction is a good supplement to functional food ingredients. Nutritional factors such as essential amino acids, essential fatty acids, dietary fiber, and mineral content suggest that the pressed cake with sacha inchi seeds could be a useful ingredient for human consumption [52, 53].

#### 3.4.1 Protein hydrolysate preparation from sacha inchi pressed cake

Protein concentrate (PC) was prepared (Figure 15), and their hydrolysate (PH) was hydrolyzed by crude papain and Calotropis proteases according to Rawdkuen et al. [53]. PC was hydrolyzed by crude papain (PH-P), and Calotropis proteases (PH-C) had a degree of hydrolysis (DH) of 2.7 and 11.2%, respectively. PH-P contained a higher amount of essential amino acids (474 g/kg) than PH-C (410 g/ kg). The protein patterns of PC and PHs by using SDS-PAGE showed the molecular weights between <8 and 57 kDa. DPPH scavenging activity and FRAP assay showed a rising PH when the DH was increased. Sacha inchi protein hydrolysates can be produced in a cost-effective way by using natural crude enzyme extracts.

Some applications of protein concentrate in food model system were performed in pork sausage and fish finger (Figure 16). The results showed that when the concentration of protein concentrate increased, the textural properties of the sausage were improved. In addition, with fried fish finger can extent it shelf life when protein hydrolysates was added when compared with the control treatment. Both hydrolysates showed effective bioactivities as well as high nutritional value (Lys, Phe, and Tyr) after in vitro gastrointestinal digestion. Thus, crude papain extracted from papaya peel and Calotropis proteases from latex can be used as alternative natural enzymes for production of protein hydrolysates by using sacha inchi meal as a starting material [53].

#### 3.4.2 Extraction and isolation of protein form rice bran

A plenty of rice bran are produced as by-products during rice milling process nowadays following the demand in production and consumption. After rice milling process, 70% rice is obtained, while rice hull (20%), rice bran (8%), and rice germ (2%) are the by-products. The major portion of this is used as animal feed or discarded as waste material [54]. However, rice bran is attracting attention from

Application of protein concentrate from sacha inchi defatted meal in fish fingers. Source: Rawdkuen et al. [53]. (a) and (b) represent fish finger contained sacha inchi protein concentrate before and after frying, respectively.

Preparation of protein concentrate from pressed cake sacha inchi. Source: Rawdkuen et al. [53].

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

DOI: http://dx.doi.org/10.5772/intechopen.89289

Figure 15.

Figure 16.

93

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289

Figure 15.

showed good sources for bioactive compounds with high antioxidant activities. Therefore, the chemical properties of the pressed cakes indicate that this byproduct of oil extraction is a good supplement to functional food ingredients. Nutritional factors such as essential amino acids, essential fatty acids, dietary fiber, and mineral content suggest that the pressed cake with sacha inchi seeds could be a

Some utilization of pressed cake from sacha inchi and tea seeds. Source: Rawdkuen et al. [53]. A and B represent Electrophoresis patterns of protein and glycoprotein of pressed-cake from tea and sacha inchi seeds,

Protein concentrate (PC) was prepared (Figure 15), and their hydrolysate (PH) was hydrolyzed by crude papain and Calotropis proteases according to Rawdkuen et al. [53]. PC was hydrolyzed by crude papain (PH-P), and Calotropis proteases (PH-C) had a degree of hydrolysis (DH) of 2.7 and 11.2%, respectively. PH-P contained a higher amount of essential amino acids (474 g/kg) than PH-C (410 g/ kg). The protein patterns of PC and PHs by using SDS-PAGE showed the molecular weights between <8 and 57 kDa. DPPH scavenging activity and FRAP assay showed a rising PH when the DH was increased. Sacha inchi protein hydrolysates can be produced in a cost-effective way by using natural crude enzyme extracts.

Some applications of protein concentrate in food model system were performed

A plenty of rice bran are produced as by-products during rice milling process nowadays following the demand in production and consumption. After rice milling

in pork sausage and fish finger (Figure 16). The results showed that when the concentration of protein concentrate increased, the textural properties of the sausage were improved. In addition, with fried fish finger can extent it shelf life when protein hydrolysates was added when compared with the control treatment. Both hydrolysates showed effective bioactivities as well as high nutritional value (Lys, Phe, and Tyr) after in vitro gastrointestinal digestion. Thus, crude papain extracted from papaya peel and Calotropis proteases from latex can be used as alternative natural enzymes for production of protein hydrolysates by using sacha inchi meal as

useful ingredient for human consumption [52, 53].

Food Preservation and Waste Exploitation

3.4.2 Extraction and isolation of protein form rice bran

a starting material [53].

92

Figure 14.

respectively.

3.4.1 Protein hydrolysate preparation from sacha inchi pressed cake

Preparation of protein concentrate from pressed cake sacha inchi. Source: Rawdkuen et al. [53].

Application of protein concentrate from sacha inchi defatted meal in fish fingers. Source: Rawdkuen et al. [53]. (a) and (b) represent fish finger contained sacha inchi protein concentrate before and after frying, respectively.

process, 70% rice is obtained, while rice hull (20%), rice bran (8%), and rice germ (2%) are the by-products. The major portion of this is used as animal feed or discarded as waste material [54]. However, rice bran is attracting attention from

researchers because it is an alternative and economic source of plant-based hypoallergenic and high-quality protein [54, 55]. Besides, rice bran is a rich source of protein, fat, carbohydrate, vitamins, minerals, dietary fiber, and bioactive compound antioxidants. Furthermore, the chemical, functional, and biofunction properties of rice protein seem superior to other proteins such as soya flake, potato starch, peanut, sorghum, kidney bean, and groundnut [55, 56]. Thus, rice bran has a strong potential to extract certain bioactive compounds, that is, protein concentrate, protein hydrolysates, active peptides, and utilization of those compounds, that is, film production (Figure 17).

applied in nutraceutical products and also cosmetic goods [58]. Thus, the several methods to extract protein from rice bran fraction are of interest and developed

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

Different extraction methods both conventional extraction such as solvent extraction [59, 60], alkaline extraction [57, 58] as well as innovative ones such as supercritical fluid [61], microwave [62], ultra-sonication [63] and enzymatic extraction [64] are used to extract protein from rice bran. Conventional method like alkaline extraction is the most common method for extracting protein from plant materials due to its simplicity and low cost. However, severe alkaline conditions negatively affect the nutritional and functional properties of the protein. This process also requires a long time for extraction and consumes large volumes of buffer. Moreover, exposing protein to severe alkaline conditions also affects the nutritional and functional properties of protein [65]. Therefore, other methods such as physical, that is, microwave- and ultrasonic-assisted extraction, as well as enzymatic

Nowadays, microwave and ultrasonic are commonly applicable methods in food

Phongthai et al. [63] studied of extraction of protein from organic rice bran by three different methods such as alkaline [58], microwave [62], ultra-sonicated extraction [63], enzyme-assisted extraction (EAE) of rice bran protein [64] and then that protein concentrate was isolated by three-phase partitioning (TPP) techniques [66] or utilization of protein concentrate as raw material for production of protein hydrolysate [58], gluten free bread [58] and pasta [67] as depicted Figure 18.

Rice barn protein extraction by conventional alkaline extraction method. Source: Phongthai et al. [58].

preparation, especially for solid-liquid extraction. It is high reproducibility in a shorter time, convenience, and less solvent consumption. Response surface methodology could be used instead of other methods that test only one variable at a time, which is time-consuming and not cost-effective. It also provides insight into the interactions of the variables and calculates the optimal response with a limited number of experiments. The integration of ultrasonic and RSM is challenging but beneficial because it could create a systematic, practical, and economical method for

through physical, chemical, and enzymatic treatments.

DOI: http://dx.doi.org/10.5772/intechopen.89289

methods are increasingly being considered as alternative methods.

rice bran protein extraction.

Figure 18.

95

Rice bran fraction is the main source of protein in rice grain. The protein content ranges from 10 to 16% (W/W) depending on its cultivars [57]. Rice bran is an economic source of high-quality plant-based protein that can exhibit an excellent functional properties and interesting bio-functions. Rice bran protein is suggested as one of the important plant-based proteins that can be applied or used as ingredient in many products such as infant food, meat ball, noodles, biscuit, breads, and gluten-free (GF) products. Moreover, its hydrolysate form has a potential to be

Different methods for extraction of protein from rice bran. Sources: Phongthai et al. [62], Phongthai et al. [63], and Phongthai et al. [64].

#### Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289

applied in nutraceutical products and also cosmetic goods [58]. Thus, the several methods to extract protein from rice bran fraction are of interest and developed through physical, chemical, and enzymatic treatments.

Different extraction methods both conventional extraction such as solvent extraction [59, 60], alkaline extraction [57, 58] as well as innovative ones such as supercritical fluid [61], microwave [62], ultra-sonication [63] and enzymatic extraction [64] are used to extract protein from rice bran. Conventional method like alkaline extraction is the most common method for extracting protein from plant materials due to its simplicity and low cost. However, severe alkaline conditions negatively affect the nutritional and functional properties of the protein. This process also requires a long time for extraction and consumes large volumes of buffer. Moreover, exposing protein to severe alkaline conditions also affects the nutritional and functional properties of protein [65]. Therefore, other methods such as physical, that is, microwave- and ultrasonic-assisted extraction, as well as enzymatic methods are increasingly being considered as alternative methods.

Nowadays, microwave and ultrasonic are commonly applicable methods in food preparation, especially for solid-liquid extraction. It is high reproducibility in a shorter time, convenience, and less solvent consumption. Response surface methodology could be used instead of other methods that test only one variable at a time, which is time-consuming and not cost-effective. It also provides insight into the interactions of the variables and calculates the optimal response with a limited number of experiments. The integration of ultrasonic and RSM is challenging but beneficial because it could create a systematic, practical, and economical method for rice bran protein extraction.

Phongthai et al. [63] studied of extraction of protein from organic rice bran by three different methods such as alkaline [58], microwave [62], ultra-sonicated extraction [63], enzyme-assisted extraction (EAE) of rice bran protein [64] and then that protein concentrate was isolated by three-phase partitioning (TPP) techniques [66] or utilization of protein concentrate as raw material for production of protein hydrolysate [58], gluten free bread [58] and pasta [67] as depicted Figure 18.

Figure 18. Rice barn protein extraction by conventional alkaline extraction method. Source: Phongthai et al. [58].

researchers because it is an alternative and economic source of plant-based hypoallergenic and high-quality protein [54, 55]. Besides, rice bran is a rich source of protein, fat, carbohydrate, vitamins, minerals, dietary fiber, and bioactive compound antioxidants. Furthermore, the chemical, functional, and biofunction properties of rice protein seem superior to other proteins such as soya flake, potato starch, peanut, sorghum, kidney bean, and groundnut [55, 56]. Thus, rice bran has a strong potential to extract certain bioactive compounds, that is, protein concentrate, protein hydrolysates, active peptides, and utilization of those compounds,

Rice bran fraction is the main source of protein in rice grain. The protein content

Different methods for extraction of protein from rice bran. Sources: Phongthai et al. [62], Phongthai et al. [63],

ranges from 10 to 16% (W/W) depending on its cultivars [57]. Rice bran is an economic source of high-quality plant-based protein that can exhibit an excellent functional properties and interesting bio-functions. Rice bran protein is suggested as one of the important plant-based proteins that can be applied or used as ingredient in many products such as infant food, meat ball, noodles, biscuit, breads, and gluten-free (GF) products. Moreover, its hydrolysate form has a potential to be

that is, film production (Figure 17).

Food Preservation and Waste Exploitation

Figure 17.

94

and Phongthai et al. [64].

#### 3.4.2.1 Alkaline extraction (ALK)

Alkaline extraction is the most common method for extracting protein from plant materials due to its simplicity and low cost. Phongthai et al. [58] studied the protein extraction from organic rice bran using alkaline extraction method which is shown in Figure 18. For alkaline extraction, DFRB was dispersed in distilled water (1:10, w/v), and the pH was adjusted to 10 using 3 M sodium carbonate. The mixture was stirred using a pilot-scale stirrer at a temperature of 50°C for 1 h. After centrifugation at 10,000 g for 10 min, the supernatant was collected and adjusted to pH 4.5 using 3 M citric acid and centrifuged at the same conditions. The dried powder is referred to as rice bran protein concentrate (RBPC). From the study, the pilot scale gained RBPC showed a yield of 7.44 g/100 g based on DFRB weight. Compared with lab scale extraction, the used pilot scale procedure gained much higher yields, 1.6–2.7-fold more protein. The main composition of RBPC was protein with 68.07 0.54% dm, which can be labeled as protein concentrate due to the protein content of more than 60% [58]. In addition, two step statistical design (1) fractional factorial design 24-1 (independent variables: X1 (pH: 8, 9, 10), X2 (temperature: 25, 35, and 45°C), X3 (stirring speed: 80, 100, and 120 rpm) and X4 (stirring time: 60, 120, and 180 min) and (2) central composite rotatable design with variables X1 (temperature: 35, 38, 45, 52, and 55°C) and X2 (stirring time: 120, 146, 210, 274, and 300 min) was used to extract rice bran protein [57]. However, with this experimental design provided only 48.53% of protein content at pH 10.0, 80 rpm, 300 min of stirring time, and 52°C, with an extraction yield of 34.51%.

#### 3.4.2.2 Microwave-assisted extraction (MAE)

Rice bran was extracted for protein by MAE using RSM, and a three-level threefactor Box-Behnken design was chosen to evaluate the effect of microwave power (X1), extraction time (X2), and solid-liquid ratio (X3) as shown in Figure 19 [62]. It was found that the optimal condition was 1000 W of microwave power, 90 s of extraction time, and a solid-liquid ratio of 0.89 g rice bran/10 mL of distilled water. The protein yield of MAE showed higher than that of ALK by about 1.54-fold [62]. The ratio of rice bran per water as 1:10 w/v and the temperature controlled at 40°C by a sensor inserted in a control vessel during 90 s of process time, generating a power range of 350–400 W, showed 11% yield and 75% protein content [68]. In the study of Bandyopadhyay et al. [69], the same ration of rice bran solution sample (100 mL) was simultaneously exposed at a frequency of 2450 MHz and operated at 800 W. The result showed that only 40 s of microwave treatment could give the protein recovery of 78.4% as against 28.9% after 1 min for conventional boiling.

#### 3.4.2.3 Ultrasonic-assisted extraction (UAE)

Another alternative method like UAE was used by Phongthai et al. [63] to study the condition of sonication amplitude (X1, 50–90%), extraction time (X2, 10– 30 min), and solid-liquid ratio (X3, 0.5–1.5 g RB/10 mL) for the extraction of protein, as depicted in Figure 20. The optimal condition for rice bran protein production was 76% sonication amplitude, 18 min extraction, and 0.99 g/10 mL solid-liquid ratio, which gave a protein yield of 4.73 0.03%. It was found that the sonication amplitude and extraction time influenced a protein yield during UAE. The optimized UAE was a more effective method than alkaline extraction method [63]. The ratio of rice bran per water as 1:10 w/v showed 11.40% yield and 73.80% protein content when using UAE [68].

Figure 20.

97

Figure 19.

Rice barn protein extraction by ultrasonic-assisted extraction method. Source: Phongthai et al. [63].

Rice bran protein extraction by microwave-assisted extraction method. Source: Phongthai et al. [62].

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

DOI: http://dx.doi.org/10.5772/intechopen.89289

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289


Figure 19.

3.4.2.1 Alkaline extraction (ALK)

Food Preservation and Waste Exploitation

3.4.2.2 Microwave-assisted extraction (MAE)

3.4.2.3 Ultrasonic-assisted extraction (UAE)

protein content when using UAE [68].

96

Alkaline extraction is the most common method for extracting protein from plant materials due to its simplicity and low cost. Phongthai et al. [58] studied the protein extraction from organic rice bran using alkaline extraction method which is shown in Figure 18. For alkaline extraction, DFRB was dispersed in distilled water (1:10, w/v), and the pH was adjusted to 10 using 3 M sodium carbonate. The mixture was stirred using a pilot-scale stirrer at a temperature of 50°C for 1 h. After centrifugation at 10,000 g for 10 min, the supernatant was collected and adjusted to pH 4.5 using 3 M citric acid and centrifuged at the same conditions. The dried powder is referred to as rice bran protein concentrate (RBPC). From the study, the pilot scale gained RBPC showed a yield of 7.44 g/100 g based on DFRB weight. Compared with lab scale extraction, the used pilot scale procedure gained much higher yields, 1.6–2.7-fold more protein. The main composition of RBPC was protein with 68.07 0.54% dm, which can be labeled as protein concentrate due to the protein content of more than 60% [58]. In addition, two step statistical design (1) fractional factorial design 24-1 (independent variables: X1 (pH: 8, 9, 10), X2

(temperature: 25, 35, and 45°C), X3 (stirring speed: 80, 100, and 120 rpm) and X4 (stirring time: 60, 120, and 180 min) and (2) central composite rotatable design with variables X1 (temperature: 35, 38, 45, 52, and 55°C) and X2 (stirring time: 120, 146, 210, 274, and 300 min) was used to extract rice bran protein [57]. However, with this experimental design provided only 48.53% of protein content at pH 10.0, 80 rpm, 300 min of stirring time, and 52°C, with an extraction yield of 34.51%.

Rice bran was extracted for protein by MAE using RSM, and a three-level threefactor Box-Behnken design was chosen to evaluate the effect of microwave power (X1), extraction time (X2), and solid-liquid ratio (X3) as shown in Figure 19 [62]. It was found that the optimal condition was 1000 W of microwave power, 90 s of extraction time, and a solid-liquid ratio of 0.89 g rice bran/10 mL of distilled water. The protein yield of MAE showed higher than that of ALK by about 1.54-fold [62]. The ratio of rice bran per water as 1:10 w/v and the temperature controlled at 40°C by a sensor inserted in a control vessel during 90 s of process time, generating a power range of 350–400 W, showed 11% yield and 75% protein content [68]. In the study of Bandyopadhyay et al. [69], the same ration of rice bran solution sample (100 mL) was simultaneously exposed at a frequency of 2450 MHz and operated at 800 W. The result showed that only 40 s of microwave treatment could give the protein recovery of 78.4% as against 28.9% after 1 min for conventional boiling.

Another alternative method like UAE was used by Phongthai et al. [63] to study

the condition of sonication amplitude (X1, 50–90%), extraction time (X2, 10– 30 min), and solid-liquid ratio (X3, 0.5–1.5 g RB/10 mL) for the extraction of protein, as depicted in Figure 20. The optimal condition for rice bran protein production was 76% sonication amplitude, 18 min extraction, and 0.99 g/10 mL solid-liquid ratio, which gave a protein yield of 4.73 0.03%. It was found that the sonication amplitude and extraction time influenced a protein yield during UAE. The optimized UAE was a more effective method than alkaline extraction method [63]. The ratio of rice bran per water as 1:10 w/v showed 11.40% yield and 73.80%

Rice bran protein extraction by microwave-assisted extraction method. Source: Phongthai et al. [62].


Figure 20. Rice barn protein extraction by ultrasonic-assisted extraction method. Source: Phongthai et al. [63].

#### 3.4.2.4 Enzyme-assisted extraction (EAE)

Phongthai et al. [64] studied the single study of variables, including solid-liquid ratio (0.5–2 g DFRB/10 mL), extraction time (2 h), and enzyme concentration (5000 units) in rice bran protein extraction. The results showed that the use of solid-liquid ratio at 0.5:10 and 1.0:10 for rice bran protein extraction gave comparable protein recovery and protein yield, reaching maximum values of 12.06 and 2.78%, respectively (extraction time 3 h and enzyme concentration 2500 U). The protein recovery and protein yield increased to 14.20–14.75% and 3.28–3.35%, respectively, when the extraction time extended to 4–5 h. These values increased as the concentration of enzymes increased. Protein recovery and protein yield reached the maximum values of 16.69 � 0.58% and 3.70 � 0.12%, respectively, when 10,000 U of enzyme was used [64]. The yield of RBPC prepared by alkaline extraction followed by acidic precipitation is 10.2%, which is further increased to 14.5 and 22.4% by papain- and Viscozyme-assisted extraction, respectively [69].

specific volume. The properties of GF bread was improved in terms of specific volume, pore size and uniformity, gas retention, and shelf life by addition of 2%

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

RBPC displayed an important anti-staling effect in GF bread. The GF bread containing 1% RBPC and 1% egg albumin had an intermediate staling rate during storage, indicating a slower rate for development of crumb hardness than GF breads enriched with 2% egg albumin and the control recipe. Additionally, crumb porosity and sensory attributes were improved. RBPC also showed higher efficacy to inhibit bread staling than egg albumin. From this study suggested that RBPC could be used

as a protein source as well as extending its shelf life for GF bread [58].

Since manufacturers have long complained about gluten-free pasta for its apparent low cooking properties and reduced nutritional value, Phongthai et al. proposed to develop a multi-sourced protein-enriched gluten-free pasta [68] as depicted in Figure 23. RBPC is one of the alternative protein sources used in this study. Egg albumen (EB), whey protein concentrates (WP), and soy protein concentrates (SPC) were all enriched into rice flour-based gluten-free pasta in order to test their level of enhancement of cooking properties. In uncooked pasta, the RBPC-

enriched pasta contained a soluble protein with a protein solubility of 22.9– 27.59 mg/g sample when compared with that of WB 40.77–50.28 mg/g sample, EB (28.40–36.77 mg/g sample), and SP (16.56–21.18 mg/g sample), respectively. However, rice bran protein did not provide satisfied quality of gluten-free pasta. Obviously, RBPC influenced the highest solid loss, which was more than 7.5%. This may

Gluten-free breads enriched with different levels of egg albumin and RBPC and sensory evaluation attributes of

3.4.3.2 Gluten-free pasta production

DOI: http://dx.doi.org/10.5772/intechopen.89289

For sensory evaluation and attributes, GF bread was determined by those attributes compared with the control bread as shown in Figure 22. High improvements in terms of appearance, color, smell, and overall liking were observed by 2% RBPC enrichment. In addition, the texture of GF breads (2% RBPC) was extremely accepted by panels, which is related to the firmness and relative elasticity values of 15.00 N and 54.11%, respectively. Taste was not significantly different between the breads containing egg albumin or RBPC. It was clearly seen that the addition of 2%

RBPC [58].

Figure 22.

99

GF breads. Source: Phongthai et al. [58].

#### 3.4.3 Application of isolated rice bran protein

#### 3.4.3.1 Bread preparation

Gluten-free-based products have been studied for several years especially for quality improvement by enriching with proteins. Besides, the allergenic character of common protein source like egg albumin is a limitation factor. Phongthai et al. [58] studied the replacement of egg albumin with rice bran protein concentrate which is a nonallergy protein in order to improve the quality of GF bread. The obtained RBPC was composed of 68.07% protein (dry basis) preparation from alkaline-acid extraction technique. The summary of GF breads enriched with different levels of egg albumin and RBPC is depicted in Figure 21. The addition of RBPC had strongly influenced the rheological properties, especially elastic modulus (G<sup>0</sup> ) of GF batters during oscillation and the relative elasticity of final GF breads. Breads enriched with 2% RBPC and a combination of 1% egg albumin and 1% RBPC had the highest

Figure 21. GF breads enriched with different levels of egg albumin and RBPC preparation. Source: Phongthai et al. [58].

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289

specific volume. The properties of GF bread was improved in terms of specific volume, pore size and uniformity, gas retention, and shelf life by addition of 2% RBPC [58].

For sensory evaluation and attributes, GF bread was determined by those attributes compared with the control bread as shown in Figure 22. High improvements in terms of appearance, color, smell, and overall liking were observed by 2% RBPC enrichment. In addition, the texture of GF breads (2% RBPC) was extremely accepted by panels, which is related to the firmness and relative elasticity values of 15.00 N and 54.11%, respectively. Taste was not significantly different between the breads containing egg albumin or RBPC. It was clearly seen that the addition of 2% RBPC displayed an important anti-staling effect in GF bread. The GF bread containing 1% RBPC and 1% egg albumin had an intermediate staling rate during storage, indicating a slower rate for development of crumb hardness than GF breads enriched with 2% egg albumin and the control recipe. Additionally, crumb porosity and sensory attributes were improved. RBPC also showed higher efficacy to inhibit bread staling than egg albumin. From this study suggested that RBPC could be used as a protein source as well as extending its shelf life for GF bread [58].

#### 3.4.3.2 Gluten-free pasta production

3.4.2.4 Enzyme-assisted extraction (EAE)

Food Preservation and Waste Exploitation

3.4.3 Application of isolated rice bran protein

3.4.3.1 Bread preparation

Figure 21.

98

Phongthai et al. [64] studied the single study of variables, including solid-liquid

Gluten-free-based products have been studied for several years especially for quality improvement by enriching with proteins. Besides, the allergenic character of common protein source like egg albumin is a limitation factor. Phongthai et al. [58] studied the replacement of egg albumin with rice bran protein concentrate which is a nonallergy protein in order to improve the quality of GF bread. The obtained RBPC was composed of 68.07% protein (dry basis) preparation from alkaline-acid extraction technique. The summary of GF breads enriched with different levels of egg albumin and RBPC is depicted in Figure 21. The addition of RBPC had strongly

during oscillation and the relative elasticity of final GF breads. Breads enriched with 2% RBPC and a combination of 1% egg albumin and 1% RBPC had the highest

GF breads enriched with different levels of egg albumin and RBPC preparation. Source: Phongthai et al. [58].

) of GF batters

influenced the rheological properties, especially elastic modulus (G<sup>0</sup>

ratio (0.5–2 g DFRB/10 mL), extraction time (2 h), and enzyme concentration (5000 units) in rice bran protein extraction. The results showed that the use of solid-liquid ratio at 0.5:10 and 1.0:10 for rice bran protein extraction gave comparable protein recovery and protein yield, reaching maximum values of 12.06 and 2.78%, respectively (extraction time 3 h and enzyme concentration 2500 U). The protein recovery and protein yield increased to 14.20–14.75% and 3.28–3.35%, respectively, when the extraction time extended to 4–5 h. These values increased as the concentration of enzymes increased. Protein recovery and protein yield reached the maximum values of 16.69 � 0.58% and 3.70 � 0.12%, respectively, when 10,000 U of enzyme was used [64]. The yield of RBPC prepared by alkaline extraction followed by acidic precipitation is 10.2%, which is further increased to 14.5 and 22.4% by papain- and Viscozyme-assisted extraction, respectively [69].

> Since manufacturers have long complained about gluten-free pasta for its apparent low cooking properties and reduced nutritional value, Phongthai et al. proposed to develop a multi-sourced protein-enriched gluten-free pasta [68] as depicted in Figure 23. RBPC is one of the alternative protein sources used in this study. Egg albumen (EB), whey protein concentrates (WP), and soy protein concentrates (SPC) were all enriched into rice flour-based gluten-free pasta in order to test their level of enhancement of cooking properties. In uncooked pasta, the RBPCenriched pasta contained a soluble protein with a protein solubility of 22.9– 27.59 mg/g sample when compared with that of WB 40.77–50.28 mg/g sample, EB (28.40–36.77 mg/g sample), and SP (16.56–21.18 mg/g sample), respectively. However, rice bran protein did not provide satisfied quality of gluten-free pasta. Obviously, RBPC influenced the highest solid loss, which was more than 7.5%. This may

#### Figure 22.

Gluten-free breads enriched with different levels of egg albumin and RBPC and sensory evaluation attributes of GF breads. Source: Phongthai et al. [58].

positive effect with DPPH and ABTS but negative effect on the reducing activity of peptide fractions. However, the UF fractions and the RBPH with MW between 3 and 5 kDa showed outstanding activity to chelate. The majority of peptides with m/z at 1088 was supposed to be octapeptides, containing eight amino acids; a major peptide fragment in RBPH that has the highest antioxidant activity was detected at

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

Rice bran protein hydrolysates at different degrees of hydrolysis (DH) (5.04, 10.37, and 15.04%) were obtained from RBPC from MAE and produced by Alcalase. The molecular weight (MW) of the rice bran protein concentrate and the PHs ranged between <11 and 100 kDa [62]. In addition, another RBPH was obtained from RBPC from UAE and further hydrolysis by Neutrase 0.8L and Subtilisin A [63]. The degree of hydrolysis for the rice bran protein by Subtilisin A, Actinase E, and Neutrase 0.8L was 20.03, 13.84, and 5.54%, respectively. The MWs of the isolated proteins ranged between <11 and 75 kDa. The RBPH exhibited greater scavenging activity on DPPH radical and ACE-inhibitory activity. The RBPH obtained by using Subtilisin A was efficient in reducing power and metal chelating activities. From these studies, it was found that that the partial hydrolyzed rice bran protein is more suitable for application in food systems rather than the non-

m/z 1088.

DOI: http://dx.doi.org/10.5772/intechopen.89289

hydrolyzed form [63].

Author details

Saroat Rawdkuen<sup>1</sup>

101

Chiba University, Chiba, Japan

\* and Sunantha Ketnawa<sup>2</sup>

Mae Fah Luang University, Chiang Rai, Thailand

\*Address all correspondence to: saroat@mfu.ac.th

provided the original work is properly cited.

1 Unit of Innovative Food Packaging and Biomaterials, School of Agro-Industry,

2 Postharvest and Food Engineering Laboratory, Graduate School of Horticulture,

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

#### Figure 23.

Characteristic of uncooked gluten-free pasta and scanning electron microscopy images of rice flour-based glutenfree pasta enriched with 6 and 9% of egg albumen (EB), rice bran protein concentrates, soy protein concentrates, and whey protein concentrates. Source: Phongthai et al. [68].

be because RBPC is comprised of 6.91% fiber, which would be responsible for weakening the starch network, thereby increasing cooking loss. Besides, the addition of RBPC had a great impact on color parameters as shown in Figure 23, possibly because RBPC was obviously darker than the other protein sources. Egg albumen manifested the greatest potential for improving gluten-free pasta as it gave a short cooking time, a low cooking loss, and a firm texture.

#### 3.4.3.3 Rice bran protein hydrolysates

Rice bran protein can be utilized by the production of rice bran protein hydrolysate (RBPH) by enzymatic techniques.The protein hydrolysates can be subjected to isolation and characterization for study possibility to be functional ingredient that benefits for human health. In the study of Phongthai et al. [64], RBPC that produced from alkaline extraction was used as raw material for rice bran protein hydrolysate production, and the hydrolysate was further fractionated by membrane ultrafiltration (UF, F1: molecular weight (MW) <3 kDa, F2: MW 3–5 kDa, and F3: MW 5–10 kDa) was used to fractionation of the hydrolysate [64]. Both RBPC and RBPH were digested by pepsin, and the combination of pepsin and trypsin under in vitro gastrointestinal digestion was investigated for its free amino acids (FAAs). The digestion affected the FAA content in each sample by RBPH had the highest FAAs content (34.44 mg/g) when compared with RBPC (22.40 mg/g) and pepsinhydrolysates (27.07 mg/g). For antioxidant properties, it was found that the digestion by pepsin and pepsin-trypsin increased the DPPH radical scavenging activity of RBPC by about 3.1–4.9-fold. The digestion by pepsin and pepsin-trypsin could simulate the metal chelating efficacy of RBPC by about 2.17–2.21-fold. UF had a

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289

positive effect with DPPH and ABTS but negative effect on the reducing activity of peptide fractions. However, the UF fractions and the RBPH with MW between 3 and 5 kDa showed outstanding activity to chelate. The majority of peptides with m/z at 1088 was supposed to be octapeptides, containing eight amino acids; a major peptide fragment in RBPH that has the highest antioxidant activity was detected at m/z 1088.

Rice bran protein hydrolysates at different degrees of hydrolysis (DH) (5.04, 10.37, and 15.04%) were obtained from RBPC from MAE and produced by Alcalase. The molecular weight (MW) of the rice bran protein concentrate and the PHs ranged between <11 and 100 kDa [62]. In addition, another RBPH was obtained from RBPC from UAE and further hydrolysis by Neutrase 0.8L and Subtilisin A [63]. The degree of hydrolysis for the rice bran protein by Subtilisin A, Actinase E, and Neutrase 0.8L was 20.03, 13.84, and 5.54%, respectively. The MWs of the isolated proteins ranged between <11 and 75 kDa. The RBPH exhibited greater scavenging activity on DPPH radical and ACE-inhibitory activity. The RBPH obtained by using Subtilisin A was efficient in reducing power and metal chelating activities. From these studies, it was found that that the partial hydrolyzed rice bran protein is more suitable for application in food systems rather than the nonhydrolyzed form [63].

#### Author details

be because RBPC is comprised of 6.91% fiber, which would be responsible for weakening the starch network, thereby increasing cooking loss. Besides, the addition of RBPC had a great impact on color parameters as shown in Figure 23, possibly because RBPC was obviously darker than the other protein sources. Egg albumen manifested the greatest potential for improving gluten-free pasta as it gave

free pasta enriched with 6 and 9% of egg albumen (EB), rice bran protein concentrates, soy protein

Characteristic of uncooked gluten-free pasta and scanning electron microscopy images of rice flour-based gluten-

Rice bran protein can be utilized by the production of rice bran protein hydrolysate (RBPH) by enzymatic techniques.The protein hydrolysates can be subjected to isolation and characterization for study possibility to be functional ingredient that benefits for human health. In the study of Phongthai et al. [64], RBPC that produced from alkaline extraction was used as raw material for rice bran protein hydrolysate production, and the hydrolysate was further fractionated by membrane ultrafiltration (UF, F1: molecular weight (MW) <3 kDa, F2: MW 3–5 kDa, and F3: MW 5–10 kDa) was used to fractionation of the hydrolysate [64]. Both RBPC and RBPH were digested by pepsin, and the combination of pepsin and trypsin under in vitro gastrointestinal digestion was investigated for its free amino acids (FAAs). The digestion affected the FAA content in each sample by RBPH had the highest FAAs content (34.44 mg/g) when compared with RBPC (22.40 mg/g) and pepsinhydrolysates (27.07 mg/g). For antioxidant properties, it was found that the digestion by pepsin and pepsin-trypsin increased the DPPH radical scavenging activity of RBPC by about 3.1–4.9-fold. The digestion by pepsin and pepsin-trypsin could simulate the metal chelating efficacy of RBPC by about 2.17–2.21-fold. UF had a

a short cooking time, a low cooking loss, and a firm texture.

concentrates, and whey protein concentrates. Source: Phongthai et al. [68].

3.4.3.3 Rice bran protein hydrolysates

Food Preservation and Waste Exploitation

Figure 23.

100

Saroat Rawdkuen<sup>1</sup> \* and Sunantha Ketnawa<sup>2</sup>

1 Unit of Innovative Food Packaging and Biomaterials, School of Agro-Industry, Mae Fah Luang University, Chiang Rai, Thailand

2 Postharvest and Food Engineering Laboratory, Graduate School of Horticulture, Chiba University, Chiba, Japan

\*Address all correspondence to: saroat@mfu.ac.th

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

## References

[1] Manisan V. Geography and Climatology in every Season of Various Parts in Thailand. Thailand, Bangkok: Technical Document of Meteorological Department; 1995

[2] Hays J. Agricultural in Thailand: History, Land Use, Indebted Farmers, Irrigation and Food Industies. Facts and Details [Internet]. 2014. Available from: http://factsanddetails.com/southeastasia/Thailand/sub5\_8h/entry-3319.html [Accessed: 1 August 2019]

[3] Kuneepong P. Facts and Details: Thailand. 2002. Available from: https:// www.ldd.go.th/FAO/z\_th/th.htm#plant [Accessed: 1 August 2019]

[4] Foreign Agriculture Service, U. S. D. o. A. Thailand Exporter Guide. 2019. Available from: https://gain.fas.usda. gov/Recent%20GAIN%20Publications/ Exporter%20Guide\_Bangkok\_Thailand\_ 2-21-2019.pdf [Accessed: 1 August 2019]

[5] Office of Agricultural Economics, A. S. o. T. Agricultural Production Information. 2019. Available from: http://oldweb.oae.go.th/oae\_report/ export\_import/export.php [Accessed: 1 August 2019]

[6] Pongsrihadulchai A. Thailand Agricultural Policies and Development Strategies. 2019. Available from: http:// ap.fftc.agnet.org/ap\_db.php?id=980& print=1 [Accessed: 1 August 2019]

[7] USDA. Country Fact Sheet on Food and Agriculture Policy Trends: Thailand. 2018. Available from: http:// www.fao.org/3/I8683EN/i8683en.pdf [Accessed: 1 August 2019]

[8] FAO. SAVE FOOD: Global Initiative on Food Loss and Waste Reduction. 2019. Available from: http://www.fao. org/save-food/en/ [Accessed: 1 August 2019]

[9] Ezejiofor TIN, Enebaku UE, Ogueke C. Waste to wealth-value recovery from agro-food processing wastes using biotechnology: A review. British Biotechnology Journal. 2014; 4(4):418-481

[16] Rawdkuen S, Thitipramote N, Benjakul S. Preparation and functional characterisation of fish skin gelatin and comparison with commercial gelatin. International Journal of Food Science and Technology. 2013;48(5):1093-1102

DOI: http://dx.doi.org/10.5772/intechopen.89289

[23] Terzioğlu P, Öğüt H, Kalemtaş A. Natural calcium phosphates from fish bones and their potential biomedical applications. Materials Science and Engineering: C. 2018;91:899-911

[24] Benjakul S, Mad-Ali S, Senphan T, Sookchoo P. Biocalcium powder from precooked skipjack tuna bone: Production and its characteristics. Journal of Food Biochemistry. 2017;

[25] Permpoon J, Theinhom L, Rawdkuen S. Food seasoning powder supplemented with fish bone. Journal of

Food Science and Agricultural Technology. 2016;2:1-7

[26] Caruso G. Fishery wastes and byproducts: A resource to be valorised. Journal of Fisheries Sciences. 2016;

[27] Ghaly AE, Ramakrishnan VV, Brooks MS, Budge SM, Dave D. Fish processing wastes as a potential source of proteins, amino acids, and oils: A critical review. Journal of Microbia and Biochemical Technology. 2013;5(4):

[28] Rustad T, Storrø I, Slizyte R. Possibilities for the utilisation of marine by-products. International Journal of Food Science & Technology. 2011;

[29] Gómez-Guillén MC, Giménez B, López-Caballero ME, Montero MP. Functional and bioactive properties of collagen and gelatin from alternative sources: A review. Food Hydrocolloids.

[30] Jongjareonrak A, Rawdkuen S, Chaijan M, Benjakul S, Osako K, Tanaka M. Chemical compositions and characterisation of skin gelatin from farmed giant catfish (Pangasianodon gigas). LWT-Food Science and Technology. 2010;43(1):161-165

41(6):e12412

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

10(1):12

107-129

46(10):2001-2014

2011;25(8):1813-1827

[17] Sai-Ut S, Benjakul S, Sumpavapol P, Kishimura H. Antioxidant activity of gelatin hydrolysate produced from fish skin gelatin using extracellular protease from Bacillus amyloliquefaciens H11. Journal of Food Processing and Preservation. 2015;39(4):394-403

[18] Sai-Ut S, Jongjareonrak A,

farmed giant catfish. Food and Bioprocess Technology. 2012;5(4):

1197-1205

Journal. 2013;7(1):79

Rawdkuen S. Re-extraction, recovery, and characteristics of skin gelatin from

[19] Ketnawa S, Benjakul S, Ling TC, Martínez-Alvarez O, Rawdkuen S. Enhanced recovery of alkaline protease from fish viscera by phase partitioning and its application. Chemistry Central

[20] Ketnawa S, Benjakul S, Martínez-Alvarez O, Rawdkuen S. Fish skin gelatin hydrolysates produced by visceral peptidase and bovine trypsin:

[21] Ketnawa S, Martínez-Alvarez O, Benjakul S, Rawdkuen S. Gelatin hydrolysates from farmed giant catfish skin using alkaline proteases and its antioxidative function of simulated gastro-intestinal digestion. Food Chemistry. 2016;192:34-42

[22] Ketnawa S, Martínez-Alvarez O, Gómez-Estaca J, del Carmen Gómez-Guillén M, Benjakul S, Rawdkuen S. Obtaining of functional components from cooked shrimp (Penaeus vannamei)

by enzymatic hydrolysis. Food Bioscience. 2016;15:55-63

103

Bioactivity and stability. Food Chemistry. 2017;215:383-390

[10] Rawdkuen S, Kaewprachu P. Valorization of food processing byproducts as smart food packaging materials and its application. Food Waste as a Resource. Vol 1. IntechOpen. 2019. pp. 1-29

[11] DFT. Production, value and utilization for whole inland fishery and whole marine fishery of Thailand. 2018. Available from: https://www4.fisheries. go.th/index.php/dof\_en/view\_role/11 [Accessed: 1 August 2019]

[12] Rawdkuen S, Vanabu A, Benjakul S. Recovery of proteases from the viscera of farmed giant catfish (Pangasianodon gigas) by three-phase partitioning. Process Biochemistry. 2012;47(12): 2566-2569

[13] Vannabun A, Ketnawa S, Phongthai S, Benjakul S, Rawdkuen S. Characterization of acid and alkaline proteases from viscera of farmed giant catfish. Food Bioscience. 2014;6:9-16

[14] Kangsanant S, Murkovic M, Thongraung C. Antioxidant and nitric oxide inhibitory activities of tilapia (Oreochromis niloticus) protein hydrolysate: Effect of ultrasonic pretreatment and ultrasonic-assisted enzymatic hydrolysis. International Journal of Food Science & Technology. 2014;49(8):1932-1938

[15] Rawdkuen S, Sai-Ut S, Benjakul S. Properties of gelatin films from giant catfish skin and bovine bone: A comparative study. European Food Research and Technology. 2010;231(6): 907-916

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289

[16] Rawdkuen S, Thitipramote N, Benjakul S. Preparation and functional characterisation of fish skin gelatin and comparison with commercial gelatin. International Journal of Food Science and Technology. 2013;48(5):1093-1102

References

Department; 1995

[1] Manisan V. Geography and

Climatology in every Season of Various Parts in Thailand. Thailand, Bangkok: Technical Document of Meteorological

Food Preservation and Waste Exploitation

[9] Ezejiofor TIN, Enebaku UE, Ogueke C. Waste to wealth-value recovery from agro-food processing wastes using biotechnology: A review. British Biotechnology Journal. 2014;

[10] Rawdkuen S, Kaewprachu P. Valorization of food processing byproducts as smart food packaging materials and its application. Food Waste as a Resource. Vol 1. IntechOpen.

[11] DFT. Production, value and

[Accessed: 1 August 2019]

[13] Vannabun A, Ketnawa S,

[14] Kangsanant S, Murkovic M, Thongraung C. Antioxidant and nitric oxide inhibitory activities of tilapia (Oreochromis niloticus) protein hydrolysate: Effect of ultrasonic pretreatment and ultrasonic-assisted enzymatic hydrolysis. International Journal of Food Science & Technology.

2014;49(8):1932-1938

907-916

Phongthai S, Benjakul S, Rawdkuen S. Characterization of acid and alkaline proteases from viscera of farmed giant catfish. Food Bioscience. 2014;6:9-16

[15] Rawdkuen S, Sai-Ut S, Benjakul S. Properties of gelatin films from giant catfish skin and bovine bone: A comparative study. European Food Research and Technology. 2010;231(6):

utilization for whole inland fishery and whole marine fishery of Thailand. 2018. Available from: https://www4.fisheries. go.th/index.php/dof\_en/view\_role/11

[12] Rawdkuen S, Vanabu A, Benjakul S. Recovery of proteases from the viscera of farmed giant catfish (Pangasianodon gigas) by three-phase partitioning. Process Biochemistry. 2012;47(12):

4(4):418-481

2019. pp. 1-29

2566-2569

[2] Hays J. Agricultural in Thailand: History, Land Use, Indebted Farmers, Irrigation and Food Industies. Facts and Details [Internet]. 2014. Available from: http://factsanddetails.com/southeastasia/Thailand/sub5\_8h/entry-3319.html

[3] Kuneepong P. Facts and Details: Thailand. 2002. Available from: https:// www.ldd.go.th/FAO/z\_th/th.htm#plant

[4] Foreign Agriculture Service, U. S. D. o. A. Thailand Exporter Guide. 2019. Available from: https://gain.fas.usda. gov/Recent%20GAIN%20Publications/ Exporter%20Guide\_Bangkok\_Thailand\_ 2-21-2019.pdf [Accessed: 1 August 2019]

[5] Office of Agricultural Economics, A.

S. o. T. Agricultural Production Information. 2019. Available from: http://oldweb.oae.go.th/oae\_report/ export\_import/export.php [Accessed: 1

[6] Pongsrihadulchai A. Thailand Agricultural Policies and Development Strategies. 2019. Available from: http:// ap.fftc.agnet.org/ap\_db.php?id=980& print=1 [Accessed: 1 August 2019]

[7] USDA. Country Fact Sheet on Food

Thailand. 2018. Available from: http:// www.fao.org/3/I8683EN/i8683en.pdf

[8] FAO. SAVE FOOD: Global Initiative on Food Loss and Waste Reduction. 2019. Available from: http://www.fao. org/save-food/en/ [Accessed: 1 August

and Agriculture Policy Trends:

[Accessed: 1 August 2019]

2019]

102

August 2019]

[Accessed: 1 August 2019]

[Accessed: 1 August 2019]

[17] Sai-Ut S, Benjakul S, Sumpavapol P, Kishimura H. Antioxidant activity of gelatin hydrolysate produced from fish skin gelatin using extracellular protease from Bacillus amyloliquefaciens H11. Journal of Food Processing and Preservation. 2015;39(4):394-403

[18] Sai-Ut S, Jongjareonrak A, Rawdkuen S. Re-extraction, recovery, and characteristics of skin gelatin from farmed giant catfish. Food and Bioprocess Technology. 2012;5(4): 1197-1205

[19] Ketnawa S, Benjakul S, Ling TC, Martínez-Alvarez O, Rawdkuen S. Enhanced recovery of alkaline protease from fish viscera by phase partitioning and its application. Chemistry Central Journal. 2013;7(1):79

[20] Ketnawa S, Benjakul S, Martínez-Alvarez O, Rawdkuen S. Fish skin gelatin hydrolysates produced by visceral peptidase and bovine trypsin: Bioactivity and stability. Food Chemistry. 2017;215:383-390

[21] Ketnawa S, Martínez-Alvarez O, Benjakul S, Rawdkuen S. Gelatin hydrolysates from farmed giant catfish skin using alkaline proteases and its antioxidative function of simulated gastro-intestinal digestion. Food Chemistry. 2016;192:34-42

[22] Ketnawa S, Martínez-Alvarez O, Gómez-Estaca J, del Carmen Gómez-Guillén M, Benjakul S, Rawdkuen S. Obtaining of functional components from cooked shrimp (Penaeus vannamei) by enzymatic hydrolysis. Food Bioscience. 2016;15:55-63

[23] Terzioğlu P, Öğüt H, Kalemtaş A. Natural calcium phosphates from fish bones and their potential biomedical applications. Materials Science and Engineering: C. 2018;91:899-911

[24] Benjakul S, Mad-Ali S, Senphan T, Sookchoo P. Biocalcium powder from precooked skipjack tuna bone: Production and its characteristics. Journal of Food Biochemistry. 2017; 41(6):e12412

[25] Permpoon J, Theinhom L, Rawdkuen S. Food seasoning powder supplemented with fish bone. Journal of Food Science and Agricultural Technology. 2016;2:1-7

[26] Caruso G. Fishery wastes and byproducts: A resource to be valorised. Journal of Fisheries Sciences. 2016; 10(1):12

[27] Ghaly AE, Ramakrishnan VV, Brooks MS, Budge SM, Dave D. Fish processing wastes as a potential source of proteins, amino acids, and oils: A critical review. Journal of Microbia and Biochemical Technology. 2013;5(4): 107-129

[28] Rustad T, Storrø I, Slizyte R. Possibilities for the utilisation of marine by-products. International Journal of Food Science & Technology. 2011; 46(10):2001-2014

[29] Gómez-Guillén MC, Giménez B, López-Caballero ME, Montero MP. Functional and bioactive properties of collagen and gelatin from alternative sources: A review. Food Hydrocolloids. 2011;25(8):1813-1827

[30] Jongjareonrak A, Rawdkuen S, Chaijan M, Benjakul S, Osako K, Tanaka M. Chemical compositions and characterisation of skin gelatin from farmed giant catfish (Pangasianodon gigas). LWT-Food Science and Technology. 2010;43(1):161-165

[31] Widyasari R, Rawdkuen S. Extraction and characterization of gelatin from chicken feet by acid and ultrasound assisted extraction. Food and Applied Bioscience Journal. 2014;2(1): 85-97

[32] Kaewprachu P, Osako K, Benjakul S, Tongdeesoontorn W, Rawdkuen S. Biodegradable protein-based films and their properties: A comparative study. Packaging Technology and Science. 2016;29(2):77-90

[33] Kaewprachu P, Ben Amara C, Oulahal N, Gharsallaoui A, Joly C, Tongdeesoontorn W, et al. Gelatin films with nisin and catechin for minced pork preservation. Food Packaging and Shelf Life. 2018;18:173-183

[34] Kaewprachu P, Osako K, Benjakul S, Rawdkuen S. Quality attributes of minced pork wrapped with catechinlysozyme incorporated gelatin film. Food Packaging and Shelf Life. 2015;3: 88-96

[35] Putsakum G, Lee DS, Suthiluk P, Rawdkuen S. The properties of gelatin film-neem extract and its effectiveness for preserving minced beef. Packaging Technology and Science. 2018;31(9): 611-620

[36] Kaewprachu P, Osako K, Benjakul S, Suthiluk P, Rawdkuen S. Shelf life extension for Bluefin tuna slices (Thunnus thynnus) wrapped with myofibrillar protein film incorporated with catechin-Kradon extract. Food Control. 2017;79:333-343

[37] Vichasilp C, Sai-Ut S, Benjakul S, Rawdkuen S. Effect of longan seed extract and BHT on physical and chemical properties of gelatin based film. Food Biophysics. 2014;9(3): 238-248

[38] Sai-Ut S, Benjakul S, Rawdkuen S. Retardation of lipid oxidation using gelatin film incorporated with longan

seed extract compared with BHT. Journal of Food Science and Technology. 2015;52(9):5842-5849

surface methodology. LWT-Food Science and Technology. 2015;64(2):

DOI: http://dx.doi.org/10.5772/intechopen.89289

Bran into a Super Food and Functional Food, Rijeka, Croatia. InTechOpen; 2017

[55] Phongthai S, Homthawornchoo W, Rawdkuen S. Preparation, properties and application of rice bran protein: A review. International Food Research

Journal. 2017;24(1):25-34

359-363

Extraction, Characterization, and Application of Agricultural and Food Processing By-Products

[56] Zhang HJ, Zhang H, Wang L, Guo XN. Preparation and functional properties of rice bran proteins from heat-stabilized defatted rice bran. Food Research International. 2012;47(2):

[57] Bernardi S, Corso MP, Baraldi IJ,

International Food Research Journal.

Comparative study of rice bran protein concentrate and egg albumin on glutenfree bread properties. Journal of Cereal

Colla E, Canan C. Obtaining concentrated rice bran protein by alkaline extraction and stirring– optimization of conditions.

[58] Phongthai S, D'Amico S, Schoenlechner R, Rawdkuen S.

[59] Wang C, Li D, Xu F, Hao T, Zhang M. Comparison of two methods for the extraction of fractionated rice bran protein. Journal of Chemistry.

[60] Wang C, Xu F, Li D, Zhang M. Physico-chemical and structural properties of four rice bran protein fractions based on the multiple solvent extraction method. Czech Journal of Food Sciences. 2016;33(3):283-291

[61] Chee FL, Iqbal S, Ismail M. Effects

[62] Phongthai S, Lim ST, Rawdkuen S. Optimization of microwave-assisted

of supercritical fluid extraction conditions on yield of protein from defatted rice bran. Journal of The Chemical Society of Pakistan. 2013;

2018;25(3):1133-1139

Science. 2016;72:38-45

2014;10:1-10

35(1):192

[47] Chaiwut P, Pintathong P, Rawdkuen S. Extraction and threephase partitioning behavior of proteases

from papaya peels. Process

Biochemistry. 2010;45(7):1172-1175

kernels with a response surface

2016;9(11):3032-3043

2016;55:109-117

158-163

105

[48] Rawdkuen S, Sai-Ut S, Benjakul S. Optimizing the tyrosinase inhibitory and antioxidant activity of mango seed

methodology. Food Analytical Methods.

[49] Torres-León C, Rojas R, Contreras-Esquivel JC, Serna-Cock L, Belmares-Cerda RE, Aguilar CN. Mango seed: Functional and nutritional properties. Trends in Food Science & Technology.

[50] Kittiphoom S. Utilization of mango seed. International Food Research Journal. 2012;19(4):1325-1335

[51] Agatonovic-Kustrin S, Kustrin E,

contribution to antioxidant activities and comparative assessment of phenolic content in mango pulp and peel. South African Journal of Botany. 2018;116:

[52] Rawdkuen S, Murdayanti D, Ketnawa S, Phongthai S. Chemical properties and nutritional factors of pressed-cake from tea and sacha inchi seeds. Food Bioscience. 2016;15:64-71

[54] Alauddina M, Islama J,

[53] Rawdkuen S, Rodzi N, Pinijsuwan S. Characterization of sacha inchi protein hydrolysates produced by crude papain and Calotropis proteases. LWT-Food Science and Technology. 2018;98:18-24

Shirakawaa H, Kosekib T, Komaia AM. Rice Bran as a Functional Food: An Overview of the Conversion of Rice

Morton DW. Phenolic acids

742-749

[39] Rawdkuen S, Suthiluk P, Kamhangwong D, Benjakul S. Mechanical, physico-chemical, and antimicrobial properties of gelatinbased film incorporated with catechinlysozyme. Chemistry Central Journal. 2012;6:131-140

[40] Kaewprachu P, Rungraeng N, Osako K, Rawdkuen S. Properties of fish myofibrillar protein film incorporated with catechin-Kradon extract. Food Packaging and Shelf Life. 2017;13:56-65

[41] Malde MK, Bügel S, Kristensen M, Malde K, Graff IE, Pedersen JI. Calcium from salmon and cod bone is well absorbed in young healthy men: A double-blinded randomised crossover design. Nutrition & Metabolism. 2010;7: 61-61

[42] Ketnawa S, Chaiwut P, Rawdkuen S. Aqueous two-phase extraction of bromelain from pineapple peels ('Phu Lae' cultv.) and its biochemical properties. Food Science and Biotechnology. 2011;20(5):12-19

[43] Ketnawa S, Chaiwut P, Rawdkuen S. Extraction of bromelain from pineapple peels. Food Science and Technology International. 2011;17(4):395-402

[44] Ketnawa S, Chaiwut P, Rawdkuen S. Pineapple wastes: A potential source for bromelain extraction. Food and Bioproducts Processing. 2012;90(3):385-391

[45] Ketnawa S, Rawdkuen S. Application of bromelain extract for muscle foods tenderization. Food and Nutrition Sciences. 2011;2(5):393

[46] Sai-Ut S, Benjakul S, Kraithong S, Rawdkuen S. Optimization of antioxidants and tyrosinase inhibitory activity in mango peels using response Extraction, Characterization, and Application of Agricultural and Food Processing By-Products DOI: http://dx.doi.org/10.5772/intechopen.89289

surface methodology. LWT-Food Science and Technology. 2015;64(2): 742-749

[31] Widyasari R, Rawdkuen S. Extraction and characterization of gelatin from chicken feet by acid and ultrasound assisted extraction. Food and Applied Bioscience Journal. 2014;2(1):

Food Preservation and Waste Exploitation

[32] Kaewprachu P, Osako K, Benjakul S, Tongdeesoontorn W, Rawdkuen S. Biodegradable protein-based films and their properties: A comparative study. Packaging Technology and Science.

seed extract compared with BHT. Journal of Food Science and Technology. 2015;52(9):5842-5849

[40] Kaewprachu P, Rungraeng N, Osako K, Rawdkuen S. Properties of fish myofibrillar protein film incorporated with catechin-Kradon extract. Food Packaging and Shelf Life. 2017;13:56-65

[41] Malde MK, Bügel S, Kristensen M, Malde K, Graff IE, Pedersen JI. Calcium from salmon and cod bone is well absorbed in young healthy men: A double-blinded randomised crossover design. Nutrition & Metabolism. 2010;7:

[42] Ketnawa S, Chaiwut P, Rawdkuen S. Aqueous two-phase extraction of bromelain from pineapple peels ('Phu Lae' cultv.) and its biochemical properties. Food Science and Biotechnology. 2011;20(5):12-19

[43] Ketnawa S, Chaiwut P, Rawdkuen S. Extraction of bromelain from pineapple peels. Food Science and Technology International. 2011;17(4):395-402

[44] Ketnawa S, Chaiwut P, Rawdkuen S. Pineapple wastes: A potential source for bromelain extraction. Food and Bioproducts Processing. 2012;90(3):385-391

[45] Ketnawa S, Rawdkuen S. Application of bromelain extract for muscle foods tenderization. Food and Nutrition Sciences. 2011;2(5):393

Rawdkuen S. Optimization of

[46] Sai-Ut S, Benjakul S, Kraithong S,

antioxidants and tyrosinase inhibitory activity in mango peels using response

[39] Rawdkuen S, Suthiluk P, Kamhangwong D, Benjakul S. Mechanical, physico-chemical, and antimicrobial properties of gelatinbased film incorporated with catechinlysozyme. Chemistry Central Journal.

2012;6:131-140

61-61

[33] Kaewprachu P, Ben Amara C, Oulahal N, Gharsallaoui A, Joly C, Tongdeesoontorn W, et al. Gelatin films with nisin and catechin for minced pork preservation. Food Packaging and Shelf

[34] Kaewprachu P, Osako K, Benjakul S, Rawdkuen S. Quality attributes of minced pork wrapped with catechinlysozyme incorporated gelatin film. Food Packaging and Shelf Life. 2015;3:

[35] Putsakum G, Lee DS, Suthiluk P, Rawdkuen S. The properties of gelatin film-neem extract and its effectiveness for preserving minced beef. Packaging Technology and Science. 2018;31(9):

[36] Kaewprachu P, Osako K, Benjakul S, Suthiluk P, Rawdkuen S. Shelf life extension for Bluefin tuna slices (Thunnus thynnus) wrapped with myofibrillar protein film incorporated with catechin-Kradon extract. Food

[37] Vichasilp C, Sai-Ut S, Benjakul S, Rawdkuen S. Effect of longan seed extract and BHT on physical and chemical properties of gelatin based film. Food Biophysics. 2014;9(3):

[38] Sai-Ut S, Benjakul S, Rawdkuen S. Retardation of lipid oxidation using gelatin film incorporated with longan

Control. 2017;79:333-343

85-97

2016;29(2):77-90

Life. 2018;18:173-183

88-96

611-620

238-248

104

[47] Chaiwut P, Pintathong P, Rawdkuen S. Extraction and threephase partitioning behavior of proteases from papaya peels. Process Biochemistry. 2010;45(7):1172-1175

[48] Rawdkuen S, Sai-Ut S, Benjakul S. Optimizing the tyrosinase inhibitory and antioxidant activity of mango seed kernels with a response surface methodology. Food Analytical Methods. 2016;9(11):3032-3043

[49] Torres-León C, Rojas R, Contreras-Esquivel JC, Serna-Cock L, Belmares-Cerda RE, Aguilar CN. Mango seed: Functional and nutritional properties. Trends in Food Science & Technology. 2016;55:109-117

[50] Kittiphoom S. Utilization of mango seed. International Food Research Journal. 2012;19(4):1325-1335

[51] Agatonovic-Kustrin S, Kustrin E, Morton DW. Phenolic acids contribution to antioxidant activities and comparative assessment of phenolic content in mango pulp and peel. South African Journal of Botany. 2018;116: 158-163

[52] Rawdkuen S, Murdayanti D, Ketnawa S, Phongthai S. Chemical properties and nutritional factors of pressed-cake from tea and sacha inchi seeds. Food Bioscience. 2016;15:64-71

[53] Rawdkuen S, Rodzi N, Pinijsuwan S. Characterization of sacha inchi protein hydrolysates produced by crude papain and Calotropis proteases. LWT-Food Science and Technology. 2018;98:18-24

[54] Alauddina M, Islama J, Shirakawaa H, Kosekib T, Komaia AM. Rice Bran as a Functional Food: An Overview of the Conversion of Rice

Bran into a Super Food and Functional Food, Rijeka, Croatia. InTechOpen; 2017

[55] Phongthai S, Homthawornchoo W, Rawdkuen S. Preparation, properties and application of rice bran protein: A review. International Food Research Journal. 2017;24(1):25-34

[56] Zhang HJ, Zhang H, Wang L, Guo XN. Preparation and functional properties of rice bran proteins from heat-stabilized defatted rice bran. Food Research International. 2012;47(2): 359-363

[57] Bernardi S, Corso MP, Baraldi IJ, Colla E, Canan C. Obtaining concentrated rice bran protein by alkaline extraction and stirring– optimization of conditions. International Food Research Journal. 2018;25(3):1133-1139

[58] Phongthai S, D'Amico S, Schoenlechner R, Rawdkuen S. Comparative study of rice bran protein concentrate and egg albumin on glutenfree bread properties. Journal of Cereal Science. 2016;72:38-45

[59] Wang C, Li D, Xu F, Hao T, Zhang M. Comparison of two methods for the extraction of fractionated rice bran protein. Journal of Chemistry. 2014;10:1-10

[60] Wang C, Xu F, Li D, Zhang M. Physico-chemical and structural properties of four rice bran protein fractions based on the multiple solvent extraction method. Czech Journal of Food Sciences. 2016;33(3):283-291

[61] Chee FL, Iqbal S, Ismail M. Effects of supercritical fluid extraction conditions on yield of protein from defatted rice bran. Journal of The Chemical Society of Pakistan. 2013; 35(1):192

[62] Phongthai S, Lim ST, Rawdkuen S. Optimization of microwave-assisted

extraction of rice bran protein and its hydrolysates properties. Journal of Cereal Science. 2016;70:146-154

[63] Phongthai S, Lim ST, Rawdkuen S. Ultrasonic-assisted extraction of rice bran protein using response surface methodology. Journal of Food Biochemistry. 2017;41(2):1-11

[64] Phongthai S, D'Amico S, Schoenlechner R, Homthawornchoo W, Rawdkuen S. Fractionation and antioxidant properties of rice bran protein hydrolysates stimulated by in vitro gastrointestinal digestion. Food Chemistry. 2018;240:156-164

[65] Fabian C, Ju YH. A review on rice bran protein: Its properties and extraction methods. Critical Reviews in Food Science and Nutrition. 2011;51(9): 816-827

[66] Patsanguan S, Hisaranusorn N, Phongthai S, Rawdkuen S. Rice bran protein isolates: Preparation and their physico-chemical and functional properties. Food and Applied Bioscience Journal. 2014;2(3):169-182

[67] Phongthai S, D'Amico S, Schoenlechner R, Homthawornchoo W, Rawdkuen S. Effects of protein enrichment on the properties of rice flour based gluten-free pasta. LWT— Food Science and Technology. 2017;80: 378-385

[68] Bedin S, Netto FM, Bragagnolo N, Taranto OP. Reduction of the process time in the achieve of rice bran protein through ultrasound-assisted extraction and microwave-assisted extraction. Separation Science and Technology. 2019;1:1-14

[69] Bandyopadhyay K, Chakraborty C, Barman AK. Effect of microwave and enzymatic treatment on the recovery of protein from Indian defatted rice bran meal. Journal of Oleo Science. 2012; 61(10):525-529

**107**

**Chapter 6**

**Abstract**

Application

tool to detect the food quality.

smart films, valorization

**1. Introduction**

Valorization of Food Processing

Traditional food packaging systems cannot provide any information related to the food quality during storage to consumers. Recently, the renewable resources have been considered as starting materials for making biodegradable packaging film. A variety of food processing by-products have been utilized, either alone or in mixtures, to produce packaging films with proper properties. It shows high possibility for smart biodegradable filmmaking as well as is applicable in the food industry. In order to monitor the food quality and to reduce the food loss and waste, a new packaging technology has been increasingly developed. Smart packaging refers to packaging systems which can monitor, detect, and inform about the qualities of food in real time. Indicator is the most commonly used device, which can communicate through direct visual change, especially in color. Natural extract and synthetic color are usually added into smart packaging films. However, synthetic dyes may be harmful to the consumers' health. Thus, the use of natural extract has been increased. Smart packaging films can be applied to various types of food products in order to monitor the food quality during transportation and storage. Thus, smart packaging could be used as a nondestructive

**Keywords:** biodegradable, by-products, food packaging, indicators, natural extract,

Nowadays, the food industry is continuously growing due to increasing levels of population and globalization and is providing a wide variety of food products to meet customer needs. The major food industries of the world include meat, poultry, fish and seafood, fruits and vegetables, dairy, and cereal. During food processing, a large number of by-products are thrown away into the environment, resulting in accumulation in the environment, and may also interfere with natural process of ecosystem. According to the FAO, 1.3 billion tons of foods are by-products. The global food losses and waste are strongly dependent on the kind of foods. The highest percentage of by-product is found in fruits and vegetables, plus roots and tubers (45%), followed by fish and seafood (35%), oilseed, meat, and dairy (20%),

By-Products as Smart Food

Packaging Materials and Its

*Saroat Rawdkuen and Pimonpan Kaewprachu*

#### **Chapter 6**

extraction of rice bran protein and its hydrolysates properties. Journal of Cereal Science. 2016;70:146-154

Food Preservation and Waste Exploitation

[63] Phongthai S, Lim ST, Rawdkuen S. Ultrasonic-assisted extraction of rice bran protein using response surface methodology. Journal of Food Biochemistry. 2017;41(2):1-11

Schoenlechner R, Homthawornchoo W,

[65] Fabian C, Ju YH. A review on rice bran protein: Its properties and

extraction methods. Critical Reviews in Food Science and Nutrition. 2011;51(9):

[66] Patsanguan S, Hisaranusorn N, Phongthai S, Rawdkuen S. Rice bran protein isolates: Preparation and their physico-chemical and functional

Journal. 2014;2(3):169-182

[67] Phongthai S, D'Amico S,

Rawdkuen S. Effects of protein enrichment on the properties of rice flour based gluten-free pasta. LWT— Food Science and Technology. 2017;80:

properties. Food and Applied Bioscience

Schoenlechner R, Homthawornchoo W,

[68] Bedin S, Netto FM, Bragagnolo N, Taranto OP. Reduction of the process time in the achieve of rice bran protein through ultrasound-assisted extraction and microwave-assisted extraction. Separation Science and Technology.

[69] Bandyopadhyay K, Chakraborty C, Barman AK. Effect of microwave and enzymatic treatment on the recovery of protein from Indian defatted rice bran meal. Journal of Oleo Science. 2012;

[64] Phongthai S, D'Amico S,

Rawdkuen S. Fractionation and antioxidant properties of rice bran protein hydrolysates stimulated by in vitro gastrointestinal digestion. Food

Chemistry. 2018;240:156-164

816-827

378-385

2019;1:1-14

61(10):525-529

106
