**3.12 Statistical analyses**

The data were analyzed on Microsoft Office Excel worksheet followed by one way ANOVA. All data are expressed as mean SEM. The data were considered significant if *P* < 0.05. Further, to note the level of significance between the experimental groups Duncan's multiple range post-hoc test was applied. All of the estimations were done in single lot using replicates and were repeated thrice. Analyses were done using Statistical Package for Social Sciences software version 16 for windows (SPSS, 16.0, IBM, Chicago, IL, USA) and in accordance to Brunning and Knitz [31].
