**2.3 Experimental design**

Mice were randomly allocated into three groups (groups 1–3). Each group comprised of five female mice (n = 5/group). Group 1 was treated with distilled water (vehicle treated; controls); group 2 was gavaged with 6 mg/100 g body weight/day aqueous *Cannabis* preparation; group 3 was gavaged with 12 mg/100 g bodyweight/ day aqueous *Cannabis* preparation. The mode of oral delivery of extracts were following the protocol published previously [21]. The tips used for this purpose to deliver the dose from the micro-pipette had the pointed surface cut to avoid any injury in the mouth of the mouse. The micro-pipette was used to deliver a small volume of (20 or 40 μl) dose. The study was continued for 30 days.
