**2.4 Collection of desired tissues**

Mice were weighed before the start of experiment as well as before killing. The animals were etherized to death and blood was collected from heart. Subsequently serum was separated and was stored at 20°C until biochemical estimations of total serum cholesterol and estradiol by ELISA. Both the ovaries and uterine horns were excised, blotted free of blood and fat tissues and were weighed. The ovary on one side of the animal was fixed in Bouin's fluid for histology and immunohistochemical localization of CB1 receptor. The contra-lateral ovary of each mouse was stored at 20°C until used for enzyme assays (for steroidogenesis, Caspase-3 and free radical parameters) and western blot analysis of CB1 receptor.
