**4.2 Immunohistochemistry of CB1 receptor in ovary**

CB1 receptors protein was demonstrated immunohistochemically in the ovaries of the control and *Cannabis* treated groups of mice. The immunoreactivity of CB1 receptors was mainly observed in the granulose cells of secondary follicles in the control group. There was a dose-dependent increase in the expression of the CB1 receptor in the ovarian sections. Intense staining was also observed in the degenerating follicles and oocyte (group 3). However, negative control did not show any immunostaining.
