**2.5 Antibodies and reagents**

All of the chemicals used for the present study were of analytical grade and were purchased either from Sigma Aldrich (St. Louis, MO, USA) or from Merck (Germany). For western blot analysis, polyclonal primary antibody against CB1 receptor was purchased from Affinity BioReagents (Rockford, IL, USA, Cat No. RQ4287) and horseradish peroxidase (HRP)-linked secondary antibody was purchased from Bangalore Genei Pvt. Ltd. (Bangalore, India). For immunohistochemistry (IHC), ABC Kit was purchased from ABC staining kit (Universal Elite, Vector Laboratories, Burlingame, CA). For 3β HSD and 17β HSD assays, pregnenelone was purchased from Sigma Aldrich (St. Louis, MO, USA).
