**Key points**

*Prediction of Maternal and Fetal Syndrome of Preeclampsia*

Reverse primer: (1172)

Reverse primer: (2917)

Reverse primer: (653)

Reverse primer: (462)

Reverse primer: (308)

Reverse primer: (198)

Reverse primer: (632)

Reverse primer: (801)

AGACATCCCACAATGGGCTC(1084)

TGAAAATCGAACCTTGCGGAAA(1151)

GAGGAAAAGGAAGGCCGACA(2845)

TGGCTGTTCACCAATCCATGA(2897)

GAGCAACAAGTGGTGTTCTCC(564)

AACACGCAGGACAGGTACAG(634)

GCTGCACTTTGGAGTGATCG(344)

GGGTTTGCTACAACATGGGC(443)

CGCTTCAGCTTCTGCTGC(176)

GTCCTCATCGCGGTAGTGC(290)

GTAGACCGGACTTGGGTGAC(120)

CATGGTGCTGTTGTTGGACG(179)

CCAGGAAACCTTCGGCTTCT(536)

CGGTAGAGGCAGCTCAGTTC(613)

CGTGGAAGGACTCATGACCA(703)

GGCAGGGATGATGTTCTGGA(782)

Caspase-1 Forward primer: (1065)

NLRP3 Forward primer: (2826)

IL-1β Forward primer: (544)

TNF-α Forward primer: (325)

p62 Forward primer: (159)

Beclin-1 Forward primer: (101)

LC3-II Forward primer: (517)

GAPDH Forward primer: (684)

Gene Sequence (5′-3′) Name GeneBank

CASP1 NM\_033292.3

NLRP3 NM\_004895.4

IL1B NM\_000576.2

TNF NM\_000594.3

SQSTM1 NM\_003900.4

BECN1 NM\_003766.3

MP1LC3A NM\_032514.3

GAPDH NM\_002046.4

according to manufacturer's protocol. The RT-qPCR was made using RT GoTaq-qPCR Master Mix (Promega, Madison, WI, USA), and the primer sequences used in this study are listed in **Table 1**. Each reaction was set in duplicate and the conditions for the RT-qPCR were as follows: initial denaturation at 96°C for 2 min and then 40 cycles at 95°C for 15 s and 60°C for 60 s, followed by a melting curve. Expression values of the analyzed transcripts were normalized to that of the enzyme-encoding glyceralde-

*Primers for inflammasome and autophagy proteins, cytokines, and GAPDH. Source: Gen Bank (https://www.*

The calculation of the differential expression of selected genes was carried out by the data processing method compared with a standard curve [65]. To analyze relative gene expression, we standardized the RNA expression levels in all samples

Cytokine concentrations in culture supernatants of placental explants were determined by enzyme-linked immunosorbent assay (ELISA), using Quantikine

hyde-3-phosphate dehydrogenase gene (GAPDH).

**1.6 Cytokine determination**

to that of a single RNA sample, which was set to a value of 100.

**86**

**Table 1.**

*ncbi.nlm.nih.gov/genbank).*

