**Chapter 3**

*Nanoemulsions - Properties, Fabrications and Applications*

Approved Standard-Eleventh Edition. Clinical and Laboratory Standards Institute document M02-A11. Wayne,

[67] Baker CN et al. Comparison of the E test to agar dilution, broth microdilution, and agar diffusion susceptibility testing techniques by using a special challenge set of bacteria. Journal of Clinical Microbiology. 1991;**29**(3):533-538. PMC269813/

[68] Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial

[69] MacGowan A et al. A new timekill method of assessing the relative efficacy of antimicrobial agents alone and in combination developed using a representative β-lactam, aminoglycoside and fluoroquinolone. Journal of Antimicrobial Chemotherapy. 1996;**38**(2):193-203. DOI: 10.1093/

substances. Nature Protocols. 2008;**3**(2):163-175. DOI: 10.1038/

nprot.2007.521

jac/38.2.193

PA; 2012

guidancedocuments/ucm073773.htm [Accessed: November 17, 2018]

[60] AOAC International. Official Methods of Analysis of the Official Analytical Chemists. Arlington, VA: Ed: Kenneth Helrich, AOAC Inc.; 1990.

[61] Holah JT. CEN/TC 216: Its role in producing current and future

European disinfectant testing standards. International Biodeterioration and Biodegradation. 2003;**51**:239-243. Available from: https://standards.cen. eu/dyn/www/f?p=204:7:0::::FSP\_ORG\_ ID:6197&cs=1C21F982635037747B259B

[62] Comité Européen de Normalisation. EN 14885:2006 Chemical disinfectants and antiseptics—Application of European Standards for chemical disinfectants and antiseptics. Brussels,

[63] US Environmental Protection Agency. Product Performance Test Guidelines OCSPP 810.2000: General Considerations for Testing Public Health Antimicrobial Pesticides. Guidance for Efficacy Testing. EPA 712-C-17-002. Washington, D.C. February; 2018

[64] US Environmental Protection Agency. EPA MLB SOP-MB-31: Procedure for the OECD Quantitative Method for Testing Antimicrobial Products against Spores of Clostridium difficile (ATCC 43598) on Inanimate,

Hard, Non-porous Surfaces. Washington, D.C. December; 2017

[65] Hoseinzadeh E, Makhdoumi P, Taha P, Hossini H, Pirsaheb M, Omid Rastegar S, et al. A review of available techniques for determination of nano-antimicrobials activity. Toxin Reviews. 2017;**36**(**1**):18-32. DOI: 10.1080/15569543.2016.1237527

[66] CLSI. Performance Standards for Antimicrobial Disk Susceptibility Tests;

pp. 133-146 (chapter 6)

DC2783DB513

Belgium. 2015

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