3.3.3 Determination of the effects of Xancus pyrum on bone marrow cellularity (BMC) in cisplatin treated animals

Bone marrow cellularity was done according to the method. Bone marrow was collected from the femur into the medium containing 2% serum and made into single cell suspension. The number of cells was determined using a haemocytometer and expressed as total cells determined by tryphan blue (1% in saline) exclusion method per femur [11].
