3.5 Effect of Xancus pyrum on the biochemical parameters after cisplatin administration

3.5.1 Estimation of Urea (Span Diagnostics Ltd., Surat, India)

3.5.1.1 Principle

Urea is converted quantitatively to ammonia and CO2 in the presence of urease. The ammonium ions react with the phenolic chromogen and hypocrite to give a green coloured complex. The intensity of the colour formed is measured at 578 nm and is directly proportional to the concentration of urea in test specimen [15].

$$\text{Urea} + \text{H}\_2\text{O} \to \text{ammonia} + \text{CO}\_2 \tag{7}$$

Ammonia þ phenolic chromogen þ hypochlorite ! green coloured complex (8)

#### 3.5.1.2 Reagents

Reagent 1: Urea (Enzyme reagent). Reagent 2: Urea (Chromogen reagent). Reagent 3: Urea diluents (buffer). Reagent 4: Urea standard (50 mg/dL).

#### 3.5.1.3 Working Reagent preparation

i. Transfer the contents of one vial of Reagent 1 to the bottle of Reagent 3.


#### 3.5.1.4 Procedure

Pipette into clean and dry test tubes labelled as blank (B), standard (S) & test (T):



Mix well, keep in a boiling water bath exactly for 1 min. Cool immediately

Filtrate/supernatant (from Step A) — — 2.0 mL Working standard — 0.5 mL — Purified water 0.5 mL — — Reagent 1: Picric acid 1.5 mL 1.5 mL — Reagent 2: sodium hydroxide, 0.75 N 0.5 mL 0.5 mL 0.5 mL

Mix well, allow to stand at room temperature exactly for 20 min and measure immediately the optical density of blank (B), standard (S) and test (T) against

The results are expressed in mean � standard deviation (SD). Statistical analysis was performed by using Students 't 'test. p values < 0.05 were considered to be

4.1.1.1 Effect of Xancus pyrum on relative organ weights after cisplatin administration

Body weight of each animal was taken before sacrifice, lymphoid organs such as thymus and spleen was excised, weighed and expressed as relative organ weight. The cisplatin treated animals showed high reduction in the weight of all the organs such as 0.23 � 0.02 g/100 g body weight of spleen, 0.17 � 0.01 g/100 g body weight of thymus, 3.7 � 0.19 g/100 g body weight of liver, 1.2 � 0.01 g/100 g body weight of kidney, 0.62 � 0.01 g/100 g body weight of lungs. The cisplatin treated along with Xancus pyrum mice showed a significantly increase in the weight of all the organs such as 0.34 � 0.072 g/100 g body weight of spleen, 0.23 � 0.01 g/100 g body weight of thymus, 4.84 � 0.05 g/100 g body weight of liver, 1.37 � 0.18 g/ 100 g body weight of kidney, 0.769 � 0.05 g/100 g body weight of lungs.

The p values of cisplatin treated animals along with Xancus pyrum for spleen was p < 0.01, which was less significant but for thymus, liver, kidney and lungs it was

Weight of all relative organs was increased in cisplatin treated animals by the extract administration, providing supportive evidence for Xancus pyrum extract is

<sup>O</sup>:D:std:–O:D:blank (12)

Blank (B) Standard (S) Test (T)

serum creatinine in mg=100 mL <sup>¼</sup> <sup>O</sup>:D:test � <sup>O</sup>:D:blank � <sup>3</sup>

under running tap water and centrifuge or filter.

Chemoprotective Effect of Edible Gastropod, Xancus pyrum and Its Usefulness…

Step B. Colour development.

DOI: http://dx.doi.org/10.5772/intechopen.88655

purified water at 520 nm.

Calculation:

3.6 Statistical analysis

statistically significant.

4.1.1 Xancus pyrum

61

4. Results and discussion

4.1 Chemoprotective effect of edible gastropod meat

p < 0.05 which was considered to be statistically significant.

immunostimulative. The results are given in Table 1.

