3.3.1 Experimental protocol

The animals were divided into three groups of six animals each as follows: Group 1: Normal animals, without any treatment.

Group 2: Treated animals received cisplatin alone dissolved in 1% gum acacia intraperitoneally for 10 consecutive days.

Group 3: Treated animals received Xancus pyrum (0.5 mg) methanolic extracts. Dissolved in 1% gum acacia intraperitoneally for 10 consecutive days.

## 3.3.2 Determination of the effects of on Xancus pyrum on lymphoid organ weight in cisplatin treated animals

Eighteen animals were randomly divided into 3 groups containing six animals each, one as normal, which did not receive any treatment. The second group as treated animals, treated with cisplatin alone. Third group treated with cisplatin and Xancus pyrum Three animals from each group were sacrificed at two different time intervals (7th and 11th day) by cervical dislocation. Body weight of each animal was taken before sacrifice, lymphoid organs such as thymus and spleen was excised, weighed and expressed as relative organ weight.
