**6. Conclusion**

This chapter provides scientific basis for the development of aquaculture techniques for the Hawaiian limpet *C. sandwicensis*. Several reproductive characteristics of the Hawaiian limpet *C. sandwicensis* were investigated such as natural spawning season (November to January), maturity size (above 1.5 cm shell length), and gonad development stage (5 stages), examining sexually matured of male and female animals. The second important issue is seed production. Induction of final maturation using dietary ARA/EPA ratio of 0.70 and injection of sGnRH at dose of 250 ng/g BW is recommended. Induction of spawning by using sGnRHa is an applicable technique and was the most practical method with no mortality occurring. Pelagic algal *Palova* and benthic diatom *Amphora* induced good survival rate for larval settlement and are potential algal species for commercial hatchery of larval rearing for *C. sandwicensis*. An effective method of using plastic liner and/or colander for handling and potential use for culture system of limpet was also developed. A practical commercial feed with good palatability, producing good growth performance at 35% dietary protein, 32% for carbohydrate and protein to energy (PE) ratio ranging from 87.2 to 102.9 mg/kcal could be used for commercial limpet feed.
