**Author's contributions**

X.Z. conceived the concept, designed the book chapter, wrote and critically revised the book chapter, coordinated and was responsible for the correspondence

**83**

provided the original work is properly cited.

Central South University, Changsha, China

© 2018 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

1 Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya

2 State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital,

*Application of Two-Dimensional Gel Electrophoresis in Combination with Mass Spectrometry…*

work and financial support. Z.T. participated in analysis of references and wrote

*DOI: http://dx.doi.org/10.5772/intechopen.82524*

**Acronyms and abbreviations**

DTT dithiothreitol

hPRL human prolactin hPRLRs hPRL receptors IEF isoelectric focusing IPG immobilized pH gradient IGF-1 insulin-like growth factor-1 JAKs janus-activating tyrosine kinases

BSA bovine serum albumin

ESI electrospray ionization

hGH human growth hormone

LC liquid chromatography

MS/MS tandem mass spectrometry ORFs open reading frames

PMF peptide mass fingerprint PTM post-translational modification

PVDF polyvinylidene fluoride Q-IT quadruple-ion trap

PAGE polyacrylamide gel electrophoresis

2DGE two-dimensional gel electrophoresis

Hospital, Central South University, Changsha, China

\*Address all correspondence to: yjzhan2011@gmail.com

*Mr* relative mass MS mass spectrometry

pI isoelectric point

TOF time-of-flight

Xianquan Zhan1,2\* and Tian Zhou1,2

**Author details**

GHRH growth hormone-releasing hormone

MALDI matrix-assisted laser desorption/ionization

SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis

partial manuscript.

*Application of Two-Dimensional Gel Electrophoresis in Combination with Mass Spectrometry… DOI: http://dx.doi.org/10.5772/intechopen.82524*

work and financial support. Z.T. participated in analysis of references and wrote partial manuscript.
