**2.4 Bioinformatics**

Many phosphorylation sites of hGH proteoforms were identified with MS/MS, and deamidation was found in many hGH proteoforms. The PTM sites of hPRL in human pituitary adenoma and control tissues, including phosphorylation sites, N-glycosylation sites, and O-glycosylation sites were predicted with NetPhos 3.1 Server (http://www.cbs.dtu.dk/services/NetPhos) [24, 25], NetNGlyc 1.0 Server (http://www.cbs.dtu.dk/services/NetNGlyc) [26], and NetOGlyc 4.0 Server (http:// www.cbs.dtu.dk/services/NetOGlyc) [27], respectively.
