**2. Etiopathogeny of diffuse large B-cell lymphoma**

#### **2.1 Epidemiology**

The incidence of diffuse large B-cell lymphoma (DLBCL) is increasing with age, from 0.3 cases/100,000/year in those between the ages of 35 and 39 years and 26.6/100,000/year for the 80- to 84-year-old age group [2, 3]. Mean age of diagnosis is 65 years for DLBCL and between 20 and 30 years of age for primary mediastinal subtype [4].

This type of lymphoma is more common in Caucasians than African and Asian populations, while the extranodal involvement is more frequent in European and the Far East population than the American patients. The sex ratio ranges from 1.5 to 3.5/1 in favor of men [5].

#### **2.2 Pathogeny**

DLBCL originates in mature B cells in certain stages of differentiation. Various changes occur in the B cell toward its malignant transformation, which are induced by genetic mutations. During their ontogenesis, the B cells are passing in the secondary lymphoid tissues where the antigen-dependent activation occurs. The activation and amassment of B cells in the secondary lymphoid tissue lead to the creation of the germinal center, which is critically dependent on BCL-6. If certain genetic alterations occur during lymphocyte development, then the determinatives of the neoplastic changes are settled. The type of lymphoma is determined by the stage of maturation of the B cell and by the type of anomalies that are interfering with their development and differentiation [6]. More specifically, the upregulation of B-cell lymphoma-2 (Bcl-2) protein expression and the inactivation of BCL6 which in turn blocks the apoptosis have both been observed in DLBCL.

Moreover, the elevation of the nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB) and the upregulation of the avian myelocytomatosis virus oncogene cellular homolog (c-Myc) expression, leading to an increase in B cell proliferation, have also been cited [7]. The signal pathways for the B-cell receptor (BCR) and the activation of NFkB and downregulation of the B-cell lymphoma-6 (Bcl-6) pathways have a special pathogenic significance in DLBCL [7].

#### **3. Classification and standardization**

#### **3.1 Classification**

DLBCL can originate from three types of cells and can be classified accordingly as germinal center DLBCL (GCB DLBCL), from the centroblasts of the germinal center; activated B-cell DLBCL (ABC-DLBCL), from the plasmablasts that are involved in the terminal differentiation of the B cells; and primary mediastinal large B-cell lymphoma (PMBCL), which derives from thymic B cells. This classification has a prognostic utility, as the activated B-cell subtype has an unfavorable evolution [8].

Apart from the cell-of-origin classification, several other classifications for non-Hodgkin lymphomas have been suggested during the last half-century, each with its own advantages and drawbacks: *Rappaport*, *Kiel*, *International Working Formulation (IWF)*, and *the Revised European-American Lymphoma classification (REAL).* The last classification that is currently in use has been offered by the World Health Organization (WHO) in 2001 and was last revised in 2016. According to the maturation stage in which the B cell is in, and to the type of anomalies that occur during differentiation and maturation, DLBCL presents several variants and subtypes.

The World Health Organization classification of the lymphoid neoplasms has systemized DLBCL into several subtypes, each with its own morphological, clinical, and immunohistochemical particularities. The 2016 revision of the WHO classification brought several updates concerning diagnostic and prognostic factors

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> Monoclonal B-cell lymphocytosis B-Cell prolymphocytic leukemia Splenic marginal zone lymphoma

> Hairy cell leukemia variant Lymphoplasmacytic lymphoma Waldenstrom macroglobulinemia

m heavy-chain disease g heavy-chain disease a heavy-chain disease

Plasma cell myeloma

Follicular lymphoma

Mantle cell lymphoma

EBV1 DLBCL, NOS EBV1 mucocutaneous ulcer

In situ mantle cell neoplasia

Germinal center B-cell type Activated B-cell type

Primary cutaneous DLBCL, leg type

Solitary plasmacytoma of bone Extraosseous plasmacytoma

Nodal marginal zone lymphoma

In situ follicular neoplasia

Monoclonal immunoglobulin deposition diseases

Pediatric nodal marginal zone lymphoma

Duodenal-type follicular lymphoma Pediatric-type follicular lymphoma

Large B-cell lymphoma with IRF4 rearrangement Primary cutaneous follicle center lymphoma

Diffuse large B-cell lymphoma (DLBCL), NOS

T-cell/histiocyte-rich large B-cell lymphoma

Primary DLBCL of the central nervous system (CNS)

Hairy cell leukemia

"DLBCL EBV+ of the elderly" (**Table 1**) [9].

Splenic B-cell lymphoma/leukemia, unclassifiable Splenic diffuse red pulp small B-cell lymphoma

Chronic lymphocytic leukemia/small lymphocytic lymphoma

Monoclonal gammopathy of undetermined significance (MGUS), IgM

Monoclonal gammopathy of undetermined significance (MGUS), IgG/A

Extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma)

for DLBCL, such as the acknowledgment of the prognostic role of double-hit DLBCL-NOS, which involves the double expression of MYC and BCL2, or the newly introduced designation of DLBCL EBV+ (NOS) which is replacing the old term of

#### *Diffuse Large B-Cell Lymphoma DOI: 10.5772/intechopen.85489*

*Normal and Malignant B-Cell*

3.5/1 in favor of men [5].

subtype [4].

**2.2 Pathogeny**

26.6/100,000/year for the 80- to 84-year-old age group [2, 3]. Mean age of diagnosis is 65 years for DLBCL and between 20 and 30 years of age for primary mediastinal

This type of lymphoma is more common in Caucasians than African and Asian populations, while the extranodal involvement is more frequent in European and the Far East population than the American patients. The sex ratio ranges from 1.5 to

DLBCL originates in mature B cells in certain stages of differentiation. Various

changes occur in the B cell toward its malignant transformation, which are induced by genetic mutations. During their ontogenesis, the B cells are passing in the secondary lymphoid tissues where the antigen-dependent activation occurs. The activation and amassment of B cells in the secondary lymphoid tissue lead to the creation of the germinal center, which is critically dependent on BCL-6. If certain genetic alterations occur during lymphocyte development, then the determinatives of the neoplastic changes are settled. The type of lymphoma is determined by the stage of maturation of the B cell and by the type of anomalies that are interfering with their development and differentiation [6]. More specifically, the upregulation of B-cell lymphoma-2 (Bcl-2) protein expression and the inactivation of BCL6 which in turn blocks the apoptosis have both been observed in DLBCL. Moreover, the elevation of the nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB) and the upregulation of the avian myelocytomatosis virus oncogene cellular homolog (c-Myc) expression, leading to an increase in B cell proliferation, have also been cited [7]. The signal pathways for the B-cell receptor (BCR) and the activation of NFkB and downregulation of the B-cell lymphoma-6

(Bcl-6) pathways have a special pathogenic significance in DLBCL [7].

DLBCL can originate from three types of cells and can be classified accordingly as germinal center DLBCL (GCB DLBCL), from the centroblasts of the germinal center; activated B-cell DLBCL (ABC-DLBCL), from the plasmablasts that are involved in the terminal differentiation of the B cells; and primary mediastinal large B-cell lymphoma (PMBCL), which derives from thymic B cells. This classification has a prognostic utility, as the activated B-cell subtype has an unfavorable

Apart from the cell-of-origin classification, several other classifications for non-Hodgkin lymphomas have been suggested during the last half-century, each with its own advantages and drawbacks: *Rappaport*, *Kiel*, *International Working Formulation (IWF)*, and *the Revised European-American Lymphoma classification (REAL).* The last classification that is currently in use has been offered by the World Health Organization (WHO) in 2001 and was last revised in 2016. According to the maturation stage in which the B cell is in, and to the type of anomalies that occur during differentiation and maturation, DLBCL presents several variants and subtypes. The World Health Organization classification of the lymphoid neoplasms has systemized DLBCL into several subtypes, each with its own morphological, clinical, and immunohistochemical particularities. The 2016 revision of the WHO classification brought several updates concerning diagnostic and prognostic factors

**3. Classification and standardization**

**3.1 Classification**

evolution [8].

**94**

for DLBCL, such as the acknowledgment of the prognostic role of double-hit DLBCL-NOS, which involves the double expression of MYC and BCL2, or the newly introduced designation of DLBCL EBV+ (NOS) which is replacing the old term of "DLBCL EBV+ of the elderly" (**Table 1**) [9].



#### **Table 1.**

*2016 WHO classification of mature B-cell neoplasms [9].*

#### **3.2 Variants of DLBCL and related pathologic entities**

Over time, several variants and subtypes of DLBCL have been identified, according to their clinical features, localization, and morphology, which are all included in the recent REAL and WHO classifications. Extranodal DLBCL are relatively rare, accounting for 1–5% of NHL [10]. Less than 40% of DLBCL cases are originating in extranodal sites, especially in the gastrointestinal tract but also the mediastinum, bones, central nervous system (CNS), testes, and breast [11].

#### *3.2.1 Primary mediastinal large B-cell lymphoma (PMBCL)*

Roughly 10% of the total number of DLBCL is represented by PMBCL, a subtype which originates in medullary thymic B cells and affects young women, around the age of 30 and 40 years. They clinically appear as large mediastinal tumors sometimes involving the lungs and pericardium, which can lead to local compression with superior vena cava syndrome and airway obstruction. PMBCL is positive for pan-B markers, CD23 and CD30, and is negative to CD15 and sIg. BCL6, CD10, and interferon regulatory factor 4 (IRF4) can sometimes be positive [12]. Several chromosomal abnormalities can be found, such as gains in chromosomal arm 9p and 2p corresponding to JAK2 and cREL loci [13, 14]. Because of the diagnosis confusions between PMBCL and DLBCL with secondary mediastinal involvement, the reports concerning the survival rates of the two pathologic entities have been similar [15].

More recently the improvements in diagnosis of PMBCL have allowed to observe a higher survival rate of this type of lymphoma compared to DLBCL [16]. Some variants of PMBCL, which own morphologic, immunophenotypical, and molecular resemblance with the nodular sclerosis subtype of Hodgkin lymphoma, are included in the category of B-cell lymphoma, unclassifiable, with features intermediate between DLBCL and classical Hodgkin lymphoma [9, 10, 17].

#### *3.2.2 Primary DLBCL of the central nervous system (CNS)*

Only a limited number of cases (2–3%) of cerebral neoplasms are lymphomas, the most frequent of which is DLBCL. Primary DLBCL of CNS can be both

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(B2M) gene [10].

**4.1 Clinical aspects**

*3.2.4 Primary breast lymphoma*

*3.2.5 Primary bone lymphoma (PBoL)*

BCL2, BCL6, and MYC rearrangements [10].

**4. Clinical, morphologic, and cytogenetic characteristics**

tions of chromosome 18 involving IGH/MALT1 [20].

intracranial pressure can also be found [18].

*3.2.3 Primary testicular lymphomas (PTL)*

germinal center and non-germinal center, while some genetic anomalies suggest the

The clinical manifestations usually range from neurological deficits to psychiatric symptoms, but ocular involvement, seizures, and symptoms of increased

PTL are usually DLBCL (>80%) but can also classified as follicular, plasmablastic, Burkitt, mantle cell, or plasmablastic lymphomas in a minority of cases. Usually this type of lymphoma is diagnosed in stages 1–2, while in advanced stages, it can also affect the central nervous system, the liver, the skin, the Waldeyer ring, and the lungs [19]. PTL displays a modified expression of adhesion molecules that can be correlated with extranodal involvement. In most cases (60–96%) the cell of origin analysis indicates the activated B-cell (ABC) pattern. Several mutations have been observed, such as MYD88 mutations in 70% of cases, rearrangements of forkhead box protein P1 (FOXP1), programmed death-ligand 1 (PDL 1), and class II MHC transactivator (CIITA), as well as inactivation mutations of beta-2 microglobulin

Primary breast lymphoma is a rare form of breast cancer, with cell of origin studies usually indicating an activated B-cell pattern. The cytogenetic analysis shows multiple chromosomal anomalies, such as trisomies 3 and 18 and transloca-

PBoL is a rare type of lymphoma that is confined to the bone tissue, preponderantly affecting the middle-aged male patients. It should be differentiated from primary medullar lymphoma which, unlike PBoL, does not display cortical involvement [21]. The germinal center subtype is more common, frequently displaying

DLBCL patients have a wide spectrum of clinical manifestations, a frequent nodal involvement, but also, in up to 40% of cases, extranodal symptoms (skin, digestive and central nervous system, etc.). All these clinical manifestations, together with the general signs and symptoms (such as fever, weight loss, sweating) usually indicate a more aggressive phenotype. On rare occasions, the diagnosis is incidentally established when the clinical examination detects enlarged lymph nodes with no other signs or symptoms. Medullary involvement is uncommon in the

early phases of the disease, as it is only found in less than 30% of cases [22].

(PET-CT) is considered mandatory to assess the extension of the disease.

As for the paraclinical investigations, usual blood tests consist in complete blood count, lactate dehydrogenase and uric acid determination, serology, osteomedular biopsy, and cytologic exam of the cerebrospinal fluid. Computed tomography (CT) scan or, even better, positron emission tomography–computed tomography

implication of the microenvironment as a pathogenic factor [18].

#### *Diffuse Large B-Cell Lymphoma DOI: 10.5772/intechopen.85489*

*Normal and Malignant B-Cell*

Lymphomatoid granulomatosis

Intravascular large B-cell lymphoma ALK1 large B-cell lymphoma Plasmablastic lymphoma Primary effusion lymphoma HHV81 DLBCL, NOS Burkitt lymphoma

Burkitt-like lymphoma with 11q aberration

*2016 WHO classification of mature B-cell neoplasms [9].*

High-grade B-cell lymphoma, NOS

lymphoma

**Table 1.**

DLBCL associated with chronic inflammation

Primary mediastinal (thymic) large B-cell lymphoma

**3.2 Variants of DLBCL and related pathologic entities**

High-grade B-cell lymphoma, with MYC and BCL2 and/or BCL6 rearrangements

*3.2.1 Primary mediastinal large B-cell lymphoma (PMBCL)*

Over time, several variants and subtypes of DLBCL have been identified, according to their clinical features, localization, and morphology, which are all included in the recent REAL and WHO classifications. Extranodal DLBCL are relatively rare, accounting for 1–5% of NHL [10]. Less than 40% of DLBCL cases are originating in extranodal sites, especially in the gastrointestinal tract but also the mediastinum, bones, central nervous system (CNS), testes, and breast [11].

B-cell lymphoma, unclassifiable, with features intermediate between DLBCL and classical Hodgkin

Roughly 10% of the total number of DLBCL is represented by PMBCL, a subtype which originates in medullary thymic B cells and affects young women, around the age of 30 and 40 years. They clinically appear as large mediastinal tumors sometimes involving the lungs and pericardium, which can lead to local compression with superior vena cava syndrome and airway obstruction. PMBCL is positive for pan-B markers, CD23 and CD30, and is negative to CD15 and sIg. BCL6, CD10, and interferon regulatory factor 4 (IRF4) can sometimes be positive [12]. Several chromosomal abnormalities can be found, such as gains in chromosomal arm 9p and 2p corresponding to JAK2 and cREL loci [13, 14]. Because of the diagnosis confusions between PMBCL and DLBCL with secondary mediastinal involvement, the reports concerning the survival rates of the two pathologic entities have been similar [15]. More recently the improvements in diagnosis of PMBCL have allowed to observe

a higher survival rate of this type of lymphoma compared to DLBCL [16]. Some variants of PMBCL, which own morphologic, immunophenotypical, and molecular resemblance with the nodular sclerosis subtype of Hodgkin lymphoma, are included in the category of B-cell lymphoma, unclassifiable, with features intermediate

Only a limited number of cases (2–3%) of cerebral neoplasms are lymphomas, the most frequent of which is DLBCL. Primary DLBCL of CNS can be both

between DLBCL and classical Hodgkin lymphoma [9, 10, 17].

*3.2.2 Primary DLBCL of the central nervous system (CNS)*

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germinal center and non-germinal center, while some genetic anomalies suggest the implication of the microenvironment as a pathogenic factor [18].

The clinical manifestations usually range from neurological deficits to psychiatric symptoms, but ocular involvement, seizures, and symptoms of increased intracranial pressure can also be found [18].

#### *3.2.3 Primary testicular lymphomas (PTL)*

PTL are usually DLBCL (>80%) but can also classified as follicular, plasmablastic, Burkitt, mantle cell, or plasmablastic lymphomas in a minority of cases. Usually this type of lymphoma is diagnosed in stages 1–2, while in advanced stages, it can also affect the central nervous system, the liver, the skin, the Waldeyer ring, and the lungs [19]. PTL displays a modified expression of adhesion molecules that can be correlated with extranodal involvement. In most cases (60–96%) the cell of origin analysis indicates the activated B-cell (ABC) pattern. Several mutations have been observed, such as MYD88 mutations in 70% of cases, rearrangements of forkhead box protein P1 (FOXP1), programmed death-ligand 1 (PDL 1), and class II MHC transactivator (CIITA), as well as inactivation mutations of beta-2 microglobulin (B2M) gene [10].

#### *3.2.4 Primary breast lymphoma*

Primary breast lymphoma is a rare form of breast cancer, with cell of origin studies usually indicating an activated B-cell pattern. The cytogenetic analysis shows multiple chromosomal anomalies, such as trisomies 3 and 18 and translocations of chromosome 18 involving IGH/MALT1 [20].

#### *3.2.5 Primary bone lymphoma (PBoL)*

PBoL is a rare type of lymphoma that is confined to the bone tissue, preponderantly affecting the middle-aged male patients. It should be differentiated from primary medullar lymphoma which, unlike PBoL, does not display cortical involvement [21]. The germinal center subtype is more common, frequently displaying BCL2, BCL6, and MYC rearrangements [10].

### **4. Clinical, morphologic, and cytogenetic characteristics**

#### **4.1 Clinical aspects**

DLBCL patients have a wide spectrum of clinical manifestations, a frequent nodal involvement, but also, in up to 40% of cases, extranodal symptoms (skin, digestive and central nervous system, etc.). All these clinical manifestations, together with the general signs and symptoms (such as fever, weight loss, sweating) usually indicate a more aggressive phenotype. On rare occasions, the diagnosis is incidentally established when the clinical examination detects enlarged lymph nodes with no other signs or symptoms. Medullary involvement is uncommon in the early phases of the disease, as it is only found in less than 30% of cases [22].

As for the paraclinical investigations, usual blood tests consist in complete blood count, lactate dehydrogenase and uric acid determination, serology, osteomedular biopsy, and cytologic exam of the cerebrospinal fluid. Computed tomography (CT) scan or, even better, positron emission tomography–computed tomography (PET-CT) is considered mandatory to assess the extension of the disease.

PET-CT is of great diagnostic and prognostic importance, but it is also necessary for the post-chemotherapy assessment, such that in case of negative scans at the end of chemotherapy regimen, the overall prognosis is favorable [23, 24]. The drawbacks of this technique consist of the possibility of false-positive results due to reversible thymic hyperplasia, infections, and sarcoidosis or as a consequence of hematopoietic growth factor therapy [23].

#### **4.2 Morphologic and cytogenetic characteristics**

The search for an exclusively cytologic classification of DLBCL is mostly abandoned, due to the wide observer-dependent variations in morphologic analysis and nosologic framing. The cytologic exam usually differentiates between centroblastic, immunoblastic, anaplastic, and T-cell/histiocyte-rich B-cell lymphoma.

The subtype of DLBCL is decided by the genetic anomalies that occur during B-cell differentiation and maturation process [6, 25].

Even though gene expression profiling has the best accuracy for the identification of the cell of origin in DLBCL, its numerous drawbacks—such as the diverse cellular regulating pathways, some financial limitations, etc.—make its widespread clinical use still impractical. Consequently, several surface molecules have been evaluated for the differentiation between GCB and ABC DLBCL, which prove to be consistent with gene expression profiling.

The Hans algorithm has structured DLBCL in these two main subtypes, by analyzing three essential markers: multiple myeloma 1 protein (MUM1), CD10, and bcl-6 (**Figure 1**). The same classification can be achieved by using the germinal center B cell-expressed transcript 1 (GCET1), CD10, BCL-6, MUM1, and FOXP1 biomarkers (the Tally and Choi algorithms) with a slightly better accuracy (**Figure 2**). All these algorithms are important, as the cell of origin classification tends to display a prognostic role, due to the fact that DLBCL-GCB subtype usually has a favorable prognosis compared to non-GCB subtype. To further add to the complexity of this classification, GC and ABC DLBCL subtypes also express several particularities in cell marker expression, activation pathways, and outcomes [26].

However, the detection of other cell marker expression has become a fundamental component both in establishing an accurate prognosis and in the development of an optimal therapeutic algorithm.

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function.

*Diffuse Large B-Cell Lymphoma DOI: 10.5772/intechopen.85489*

*Choi algorithm for DLBCL subtyping.*

**Figure 2.**

**5. Treatment of diffuse large B-cell lymphoma**

the 2015 ESMO guidelines [10].

The treatment protocol in DLBCL did not witness significant changes during the last two decades. The widespread adoption of rituximab as an important adjuvant to standard chemotherapy protocol in CD20+ cases was a notable exception, which provided significant improvement in disease-free survival, overall survival, and complete remission rates, with limited toxicity. However, no less than 20% of patients diagnosed with DLBCL exhibit relapse after the initial response to R-CHOP (rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone) regimen, while more than 15% of the patients exhibit primary refractory disease [27]. All these factors required the search for alternative therapeutic regimens, such as R-CHOEP (rituximab, cyclophosphamide, doxorubicin, vincristine, etoposide, and prednisone), R-miniCHOP (adjusted short-term R-CHOP therapy), R-ACVBP (adriamycin-cytoxan-vindesine-bleomycin-prednisonerituximab), etc., that are strictly codified by several therapeutic protocols, such as

The choice for any of these regimens should be adapted to the specific clinical status of each patient, as the exact therapy depends on a number of factors, such as the stage of the disease, age, and biological status. However, despite numerous

The association of radiation therapy to chemotherapy offered conflicting results. While some studies showed some benefits for the combined therapy, most of the authors did not find any real advantages, especially in the case of localized disease without bulky mass [28]. There were some suggestions of a combined therapeutic algorithm, involving four R-CHOP cycles followed by either local radiation therapy or two more cycles of R-CHOP, in the case of complete remission. Armitage et al. pleaded for mandatory radiation therapy after six cycles of R-CHOP in the case of bulky disease [29]. More recently MInT (MabThera International Trial) study recommended the same therapeutic algorithm for localized bulky disease [30].

In the case of localized disease, the therapeutic protocol varies with the specific organ involvement. For primary DLBCL of the testis, R-CHOP should be associated with radiotherapy and methotrexate or intrathecal cytarabine, due to the potential risk of CNS involvement. In case of documented CNS involvement before the onset of therapy, then high-dose intravenous and intrathecal methotrexate is advised. Conversely, cytarabine should be used instead of methotrexate for lymphomatous meningitis [31]. R-CEOP can be advised for patients with an altered cardiac

In the case of advanced stage DLBCL, the therapeutic options varied greatly

since the early 1980s. The third-generation regimens, ProMACE/CytaBOM

attempts, no clear recommendation has been published thus far.

**Figure 1.** *Hans algorithm for DLBCL subtyping.*

*Normal and Malignant B-Cell*

hematopoietic growth factor therapy [23].

consistent with gene expression profiling.

outcomes [26].

an optimal therapeutic algorithm.

**4.2 Morphologic and cytogenetic characteristics**

B-cell differentiation and maturation process [6, 25].

PET-CT is of great diagnostic and prognostic importance, but it is also necessary for the post-chemotherapy assessment, such that in case of negative scans at the end of chemotherapy regimen, the overall prognosis is favorable [23, 24]. The drawbacks of this technique consist of the possibility of false-positive results due to reversible thymic hyperplasia, infections, and sarcoidosis or as a consequence of

The search for an exclusively cytologic classification of DLBCL is mostly abandoned, due to the wide observer-dependent variations in morphologic analysis and nosologic framing. The cytologic exam usually differentiates between centroblastic,

The subtype of DLBCL is decided by the genetic anomalies that occur during

Even though gene expression profiling has the best accuracy for the identification of the cell of origin in DLBCL, its numerous drawbacks—such as the diverse cellular regulating pathways, some financial limitations, etc.—make its widespread clinical use still impractical. Consequently, several surface molecules have been evaluated for the differentiation between GCB and ABC DLBCL, which prove to be

The Hans algorithm has structured DLBCL in these two main subtypes, by analyzing three essential markers: multiple myeloma 1 protein (MUM1), CD10, and bcl-6 (**Figure 1**). The same classification can be achieved by using the germinal center B cell-expressed transcript 1 (GCET1), CD10, BCL-6, MUM1, and FOXP1 biomarkers (the Tally and Choi algorithms) with a slightly better accuracy (**Figure 2**). All these algorithms are important, as the cell of origin classification tends to display a prognostic role, due to the fact that DLBCL-GCB subtype usually has a favorable prognosis compared to non-GCB subtype. To further add to the complexity of this classification, GC and ABC DLBCL subtypes also express several particularities in cell marker expression, activation pathways, and

However, the detection of other cell marker expression has become a fundamental component both in establishing an accurate prognosis and in the development of

immunoblastic, anaplastic, and T-cell/histiocyte-rich B-cell lymphoma.

**98**

**Figure 1.**

*Hans algorithm for DLBCL subtyping.*

**Figure 2.** *Choi algorithm for DLBCL subtyping.*
