*3.6.1 Anticandidosis activities*


Activities of LSSM against staphylococci (on example of patients *S. aureus* isolates): aLL > aLB; aLL and aLB; LSSM activities accompanied with:


LSSM possess potential of diagnostics and prognostics of biotope diseases [37]. Results support important principle approach that diseases of MB (as in the case of CBF according to the principle "there is the body—there are diseases") reflect own "biotope diseases" in organism [39]. LSSM can be used in phenotyping prognostics and diagnostics of infectious processes in organism; for prophylaxis and therapy of candidoses, staphylococcoses, mixed fungal-bacterial infections, mixed symbiotic mycoses; innate infections; immunodeficiency and infections against the background of immunodeficiency, upon antibiotics-/chemo-/radiotherapy. Such GC effectors as metabiotics, prebiotics, glycoantigens, and drugs of selective directed action are of availability.

It is of importance to consider the participation of LSSM in supporting biotope mucosal MB in conditions of oxidative stress (cofunctioning of laLL and probiotic oxidoreductase systems) [26]. Panel combinations of LB and LL are perspective in early evaluation (before the inflammation) of status of MB of functionally different biotopes of the vaginal tract.

**85**

*Metabolite Multiprobiotic Formulas for Microbial Health DOI: http://dx.doi.org/10.5772/intechopen.86449*

intercellular reception in innate immunity.

**3.7 Potential of results for innovations**

antifungal action in organism.

*3.7.1 Metabolite technologies*

compounds [EPC])

their complexes)

[40, 41].

LSSM are similar (in specificities, systemic action) to communicative lectins of

LSSM act as essential part of human protective supersystem (the supersystem with probiotic type action) [31]. Such supersystems contract disturbing the balance of the body's health processes; keep and maintain the integrity of biotope microbiocenoses like healthy consortia. There are supersystems with antimicrobial or

Results obtained by us in a study of probiotics can be useful as a set of approaches in study of the following aspects of clinical microbiology and medical biotechnology (in brackets—significance and prospects of results for infectology)

• Cultural metabolites 27–200 kD within pI 4–8 (separation of acidic and alkaline protein and nonprotein of taxonomically significant systems); phenotyping of strains in cationic region containing protected sets of proteins Subcytoagglutinating activities: initiatively coupled or non-coupled to lectin and cytoagglutinating activities (cytokine activities; synchronization of

• Functional blotting analysis of cascade cultures in combinative nutrient media (NM) (as in the case of milk followed by transfer into "casein hydrolysate-yeast autolysate" media; monitoring lectins, enzymes, and exopolymeric

• Development of strain-supporting NM (comparison of blotted maps of

• The use of α-S-, β/γ-, and kappa-caseinases (increase of effectiveness of NM, accumulation of physiologically active protein and nonprotein metabolites and

• Systems of proteinases, EPC depolymerases, and/or peroxidase-like catalases in cultures (revealing strain-dependent producers of enzymes, proteins, and EPC; evaluation of cytolysis populations, oxidative stress, virulent factors,

• Lectin systems: major and minor, building and signal, *cross talk* and *quorum sensing* (standardization of cultures and analysis of communicative networks) \*Evaluation of early and prolonged landscape synergism of recognizing components of cultures and antibiotics (individual choice and replacement of

• Natural and recombinant systems oriented to recognition of polyvalent glycoconjugate targets of known structure (standardization of strains; monitoring

• Imitation by cultural metabolites in respect to strain or consortium effective-

combinations of probiotics and antibiotics upon therapy of patient)

cultures for increasing vulnerability of pathogens; typing).

changes, and aging of cultures upon passages and storage)

and revealing signals of carbohydrate metabolism)

ness (standardization of strain multifunctionality)

components of cultures in identical conditions)

LSSM are similar (in specificities, systemic action) to communicative lectins of intercellular reception in innate immunity.

LSSM act as essential part of human protective supersystem (the supersystem with probiotic type action) [31]. Such supersystems contract disturbing the balance of the body's health processes; keep and maintain the integrity of biotope microbiocenoses like healthy consortia. There are supersystems with antimicrobial or antifungal action in organism.
