**Abstract**

Advancement in technology is continued as the time is passed. The biological active ingredients technology is the challenging issues for both the researchers and manufacturers. In last decade, much research has been happened about the potential health benefits of pro and prebiotic ingredients. *Probiotics* are the live microorganisms when given in adequate amount confer health benefits but the prebiotics are indigestible ingredients that enhance the activity of one or more probiotics in the colon. The therapeutic potential of these two biological active ingredients is the outcome of emerging field of biotechnology. A number of studies indicating therapeutic potential of probiotics regarding gastrointestinal health, cancer, constipation, immunomodulation, *Helicobacter pylori*, liver disease and urogenital infection have been documented. Similarly, prebiotics also improved helpful for many communicable and non-communicable diseases. It can be seen that advancement in the biotechnology field offer great choice to deliver a marvelous health advantages. Although the market for probiotic and prebiotic food and supplements has been increased in this new era but still need to develop food and supplements containing these two active ingredients with the cooperation of legal bodies of the country.

**Keywords:** therapeutic, probiotics, prebiotics
