*7.1.3.2 Streptozotocin model of DM*

Streptozotocin prevents DNA synthesis in mammalian cells (and bacteria cells) resulting in mammalian cell death. The induction of DM with streptozotocin takes some time. Diabetes develops gradually and may be assessed after a few days, usually 4 days in mice and 7 days in rats. Single dose of streptozotocin in sterile citrate buffer may be used: rats 80 mg/kg; mice 150 mg/kg administered intraperitoneally. This may produce a serum glucose level of about 180–500 mg/dl as DM induction. Although it is the most commonly used model, problems involved in its use include spontaneous recovery from high blood glucose levels by development of functioning insulinoma [35] and high incidence of kidney and liver tumors, due to the oncogenic action of streptozotocin [36].
