**8. Topical anti-aging agent**

Antioxidant and cell regulators are the main agents which are used for antiaging action. Antioxidants such as ascorbic acid, polyphenols and flavonoids reduces the collagen degradation by reducing the concentration of free radicals, while cell regulators (retinol, peptide, growth regulators) affect the collagen metabolism and stimulate collagen production.

Ascorbic acid, tocopherol, niacinamide is low molecular weight antioxidant and they have the ability to penetrate skin. l-Ascorbic acid is water soluble photosensitive substance having anti-aging action at the concentration of 5–15% and act by inducing the production of collagen 1 and collagen 3. Several enzymes are also important in the production of collagen.

The combination of vitamin E and vitamin C is more effective in comparison to individual effect of vitamin E and vitamin C.
